NISCH (NM_007184) Human Tagged ORF Clone

CAT#: RC206026

NISCH (Myc-DDK-tagged)-Human nischarin (NISCH)

Product Images

Circular map for RC206026

• 

  Western blot validation of overexpression lysate (Cat# LY416135) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206026 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NISCH protein (Cat# TP306026). The protein was produced from HEK293T cells transfected with NISCH cDNA clone (Cat# RC206026) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NISCH
• Synonyms: hIRAS; I-1; IR1; IRAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206026 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCGCGCACCTTCGGGCCCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCT
  CGGAGCTTGTGGACACTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGTAAA
  GCACCGCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGATTGATAAAAACCTG
  CTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGAGAAGAGGGAGAAGGATCTGGAGG
  TCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGTGACCCCCAGAGTACTGGCCCACTTCTTGCATTT
  TCACTTCTATGAGATAAATGGCATCACCGCGGCACTGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTC
  CTGGGGGCCGGCGAGGTCTTTGCCATTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGG
  GAAAGCCCACGTGCGCCAGTGGGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCGCCT
  TAAGTACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGGACCAGCAACATTCAGGAGCAGCTCCTGCCG
  TTCGACCTATCAATATTCAAGTCCCTGCATCAGGTGGAGATAAGTCACTGTGATGCTAAGCACATCAGAG
  GGCTGGTCGCATCGAAGCCCACCTTAGCCACGCTGAGTGTCCGCTTCTCAGCAACCTCGATGAAGGAAGT
  CCTTGTTCCTGAAGCCTCAGAATTTGATGAGTGGGAGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACT
  GCCGTCATCCCCACTTGGCAGGCATTGACCACGCTTGACCTGAGCCACAACAGCATCTCCGAGATCGACG
  AGTCTGTGAAACTGATCCCAAAGATTGAGTTCCTGGACCTGAGTCACAATGGATTGCTGGTTGTGGACAA
  TCTGCAGCACCTGTATAACCTTGTGCATCTGGACCTGTCCTACAACAAGCTCTCCTCCTTGGAAGGGCTT
  CACACCAAGCTGGGGAACATCAAGACCTTAAACCTGGCAGGCAACCTCCTAGAGAGTCTGAGTGGCCTGC
  ACAAGCTCTACTCACTGGTCAACCTGGATCTCCGGGACAACAGGATCGAACAGATGGAGGAGGTCCGGAG
  CATAGGCAGCCTCCCGTGTCTGGAGCACGTGTCTCTGCTGAACAACCCTCTGAGCATCATCCCCGACTAC
  CGGACCAAGGTGCTGGCTCAGTTCGGAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACAGTGACCACAG
  AGAAGGAGCTGGACACTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAGGAGGTCAAGTCCAAACTGAG
  CAACCCAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCAGCTGCCCCCTGCATCAGACCCAGCAGCTCC
  CCTCCCACTGTGGCTCCCGCATCTGCCTCCCTGCCCCAGCCCATCCTCTCTAACCAAGGAATCATGTTCG
  TTCAGGAGGAGGCCCTGGCCAGCAGCCTCTCGTCCACTGACAGTCTGACTCCCGAGCACCAGCCCATTGC
  CCAGGGATGTTCTGATTCCTTGGAGTCCATCCCTGCGGGACAGGCAGCTTCCGATGATTTAAGGGACGTG
  CCAGGAGCTGTTGGTGGTGCAAGCCCAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCGGGGTCTGGCC
  AGATCATCTTCCTGCCCTTCACCTGCATTGGCTACACGGCCACCAATCAGGACTTCATCCAGCGCCTGAG
  CACACTGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCCTGGATCGAGGCTGCCAACCAGCGGGAGGAG
  GGCCAGGGTGAACAGGGCGAGGAGGAGGATGAGGAGGAGGAAGAAGAGGAGGACGTGGCTGAGAACCGCT
  ACTTTGAAATGGGGCCCCCAGACGTGGAGGAGGAGGAGGGAGGAGGCCAGGGGGAGGAAGAGGAGGAGGA
  AGAGGAGGATGAAGAGGCCGAGGAGGAGCGCCTGGCTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTC
  CTGCTGGAGCACATCCGCATCCTCAAGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGCAGTATCCGCC
  AGTTCGCCGCCTGCCTTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAGATCCCGCACCAGGAGTCTCG
  GGGCAGCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTCTTTTGCTTCCCGCATGGCGACCTCACCGAG
  TTTGGCTTCCTCATGCCGGAGCTGTGTCTGGTGCTCAAGGTACGGCACAGTGAGAACACGCTCTTCATTA
  TCTCGGACGCCGCCAACCTGCACGAGTTCCACGCGGACCTGCGCTCATGCTTTGCACCCCAGCACATGGC
  CATGCTGTGTAGCCCCATCCTCTACGGCAGCCACACCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACC
  TTCTACAAGGTGGCTGGCGGCTGCCAGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTGGTCTACAGTG
  ACAAGCGCATGGTGCAGACGGCCGCCGGGGACTACTCAGGCAACATCGAGTGGGCCAGCTGCACACTCTG
  TTCAGCCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCCGTCAAGTCCGCCGCCATCCCCTACTGGCTG
  TTGCTCACGCCCCAGCACCTCAACGTCATCAAGGCCGACTTCAACCCCATGCCCAACCGTGGCACCCACA
  ACTGTCGCAACCGCAACAGCTTCAAGCTCAGCCGTGTGCCGCTCTCCACCGTGCTGCTGGACCCCACACG
  CAGCTGTACCCAGCCTCGGGGCGCCTTTGCTGATGGCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTT
  GTCACTGCCATCTTCGTGCTGCCCCACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAGCTGCGGGCCT
  CGCTGCAGGACCTGAAGACTGTGGTCATCGCCAAGACCCCCGGGACGGGAGGCAGCCCCCAGGGCTCCTT
  TGCGGATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGACCAGCGTCCCCAGGAGGTCCCAGCAGAGGCT
  CTGGCCCCGGCCCCAGTGGAAGTCCCAGCTCCAGCCCCTGCAGCAGCCTCAGCCTCAGGCCCAGCGAAGA
  CTCCGGCCCCAGCAGAGGCCTCAACTTCAGCTTTGGTCCCAGAGGAGACGCCAGTGGAAGCTCCAGCCCC
  ACCCCCAGCCGAGGCCCCTGCCCAGTACCCGAGTGAGCACCTCATCCAGGCCACCTCGGAGGAGAATCAG
  ATCCCCCCGCACTTGCCTGCCTGCCCGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGCGCCATCATCG
  AGCTCTTCCACAGCAGCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCACCTCATGTGGTCCTCGGTGGT
  GTTCTACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTGCTGCTCTCCACCAAGGCTGTGTACTTTGTG
  CTCCACGACGGCCTCCGCCGCTACTTCTCAGAGCCACTGCAGGATTTCTGGCATCAGAAAAACACCGACT
  ACAACAACAGCCCTTTCCACATCTCCCAGTGCTTCGTGCTAAAGCTTAGTGACCTGCAGTCAGTCAATGT
  GGGGCTTTTCGACCAGCATTTCCGGCTGACGGGTTCCACCCCGATGCAGGTGGTCACGTGCTTGACGCGG
  GACAGCTACCTGACGCACTGCTTCCTCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAACGCACGCCCT
  CGCCGGAGCCTGTTGACAAGGACTTCTACTCCGAGTTTGGGAACAAGACCACAGGGAAGATGGAGAACTA
  CGAGCTGATCCACTCTAGTCGCGTCAAGTTTACCTACCCCAGTGAGGAGGAGATTGGGGACCTGACGTTC
  ACTGTGGCCCAAAAGATGGCTGAGCCAGAGAAGGCCCCAGCCCTCAGCATCCTGCTGTACGTGCAGGCCT
  TCCAGGTGGGCATGCCACCCCCTGGGTGCTGCAGGGGCCCCCTGCGCCCCAAGACACTCCTGCTCACCAG
  CTCCGAGATCTTCCTCCTGGATGAGGACTGTGTCCACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCG
  CAGAGAGACAGGTACCGGCTGGACGATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTCATGGGCTACC
  AGACCTACCCGCAGGCCCTCACCCTCGTCTTCGATGACGTGCAAGGTCATGACCTCATGGGCAGTGTCAC
  CCTGGACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCGTGAAGTCCAGTGGCAG
  GTGTTTGTCCCCAGTGCTGAGAGCAGAGAGAAGCTCATCTCGCTGTTGGCTCGCCAGTGGGAGGCCCTGT
  GTGGCCGTGAGCTGCCTGTCGAGCTCACCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206026 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKLVAERKIDKNL
  LPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQL
  LGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLP
  FDLSIFKSLHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVT
  AVIPTWQALTTLDLSHNSISEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGL
  HTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDY
  RTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSS
  PPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDV
  PGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEAANQREE
  GQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDF
  LLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTE
  FGFLMPELCLVLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLT
  FYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWL
  LLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVLELLVGYRF
  VTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADGQPAERRASNDQRPQEVPAEA
  LAPAPVEVPAPAPAAASASGPAKTPAPAEASTSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQ
  IPPHLPACPSLRHVASLRGSAIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFV
  LHDGLRRYFSEPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTR
  DSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYPSEEEIGDLTF
  TVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEIFLLDEDCVHYPLPEFAKEPP
  QRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQ
  VFVPSAESREKLISLLARQWEALCGRELPVELTG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007184
• ORF Size: 4512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007184.4
• RefSeq Size: 5252 bp
• RefSeq ORF: 4515 bp
• Locus ID: 11188
• UniProt ID: Q9Y2I1
• Cytogenetics: 3p21.1
• Domains: LRR, PX, LRR_SD22
• Protein Families: Druggable Genome
• MW: 166.7 kDa
• Gene Summary: This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor. [provided by RefSeq, Jan 2013]