MTAP (NM_002451) Human Tagged Lenti ORF Clone

CAT#: RC206024L2

  • LentiORF®

Lenti ORF clone of Human methylthioadenosine phosphorylase (MTAP), mGFP tagged


  "NM_002451" in other vectors (6)

Reconstitution Protocol

USD 600.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Forward primer to sequence targets cloned in expression vectors.
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Reverse primer to sequence targets cloned in expression vectors.
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Symbol MTAP
Synonyms BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC206024).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_002451
ORF Size 849 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002451.3
RefSeq Size 4937 bp
RefSeq ORF 852 bp
Locus ID 4507
UniProt ID Q13126
Cytogenetics 9p21.3
Domains Mtap_PNP
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
MW 31.3 kDa
Gene Summary This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.