beta COP (COPB1) (NM_016451) Human Tagged ORF Clone

CAT#: RC206009

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COPB1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit beta 1 (COPB1), transcript variant 1



  "NM_016451" in other vectors (6)

Reconstitution Protocol

USD 872.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol COPB1
Synonyms BARMACS; COPB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206009 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGCGGCTGAGAACGTATGCTACACGTTAATTAACGTGCCAATGGATTCAGAACCACCATCTGAAA
TTAGCTTAAAAAATGATCTAGAAAAAGGAGATGTAAAGTCAAAGACTGAAGCTTTGAAGAAAGTAATCAT
TATGATTCTGAATGGTGAAAAACTTCCTGGACTTCTGATGACCATCATTCGTTTTGTGCTACCTCTTCAG
GATCACACTATCAAGAAATTACTTCTGGTATTTTGGGAAATTGTTCCTAAAACAACTCCAGATGGGAGAC
TTTTACATGAGATGATCCTTGTATGTGATGCATACAGAAAGGATCTTCAACATCCTAATGAATTTATTCG
AGGATCTACTCTTCGTTTTCTTTGCAAATTGAAAGAAGCAGAATTGCTAGAACCTTTAATGCCAGCTATT
CGTGCATGTTTGGAGCATCGACACAGCTATGTTAGAAGAAATGCTGTTTTGGCCATCTATACCATCTATA
GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTGGTGAATGAGAAGGATGC
AAGTTGCAAAAGGAATGCATTTATGATGCTAATTCATGCAGATCAGGATCGAGCTTTGGATTACTTAAGT
ACTTGCATTGATCAAGTTCAAACATTTGGAGACATTCTGCAGCTGGTTATTGTTGAACTGATTTATAAGG
TCTGTCATGCTAATCCATCAGAAAGAGCTCGTTTTATTCGCTGCATCTATAACTTATTACAGTCATCCAG
CCCTGCTGTAAAATATGAAGCTGCTGGGACATTAGTGACACTCTCTAGTGCACCAACTGCAATCAAGGCT
GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGCGACAACAATGTAAAACTCATAGTTTTGGATC
GCTTGATAGAATTAAAAGAGCATCCTGCTCATGAACGAGTACTACAGGATCTGGTTATGGATATCCTAAG
AGTATTGAGCACACCAGACTTAGAAGTACGAAAGAAAACTCTGCAGTTAGCACTGGATCTTGTCTCTTCT
AGAAATGTTGAAGAGCTGGTTATTGTCCTGAAGAAGGAAGTGATAAAAACAAATAATGTGTCTGAGCATG
AAGATACTGACAAATACAGACAACTCCTAGTGCGAACATTGCATTCCTGTTCTGTCCGATTTCCAGATAT
GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAACAACGAAGCAGCAGCTGCTGATGTC
TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGATAACCTGAGAATGCTTATTGTTGAGAAGATGCTTG
AAGTCTTTCATGCTATTAAATCTGTCAAGATTTACCGAGGAGCATTATGGATCCTGGGAGAATACTGTAG
TACCAAGGAAGACATTCAGAGTGTGATGACTGAGATCCGCAGGTCCCTTGGAGAGATCCCAATTGTAGAG
TCAGAAATAAAGAAAGAAGCTGGTGAATTAAAACCTGAAGAAGAAATAACTGTAGGGCCAGTTCAGAAAT
TGGTTACTGAAATGGGTACCTATGCAACTCAGAGTGCCCTTAGCAGTTCTAGACCCACCAAGAAAGAGGA
AGACAGACCTCCCTTGAGAGGATTCCTTCTGGATGGAGATTTCTTTGTTGCTGCCTCCCTTGCCACAACT
CTGACCAAGATTGCATTGCGCTATGTAGCTTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG
AGGCTATGTTGCTCATGGCTACTATCCTGCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA
TGATGATGTGGATCGAATTTCCCTGTGCCTCAAGGTCTTGTCTGAATGTTCACCTTTAATGAATGACATT
TTCAATAAGGAATGCAGACAGTCCCTTTCTCACATGTTATCTGCTAAACTAGAAGAAGAGAAATTATCCC
AAAAGAAAGAATCTGAAAAGAGGAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC
TGCTAAGAATGAAATGAACTGCAAGGAAGATCAGTTTCAGCTGAGTTTACTGGCAGCAATGGGTAACACA
CAGAGGAAAGAGGCAGCAGATCCCCTAGCATCTAAACTTAACAAGGTCACCCAATTGACAGGTTTCTCAG
ATCCTGTATATGCAGAAGCTTACGTTCATGTCAACCAATATGATATTGTCCTGGATGTACTTGTTGTGAA
CCAAACCAGTGATACTTTGCAGAATTGCACATTAGAACTAGCTACACTAGGGGATCTGAAACTTGTGGAA
AAGCCGTCTCCTTTGACTCTTGCTCCTCATGACTTCGCAAATATTAAAGCTAACGTCAAAGTAGCATCAA
CAGAAAATGGAATAATTTTTGGTAATATAGTTTATGATGTCTCTGGAGCAGCAAGTGACAGAAATTGTGT
GGTTCTCAGTGATATTCACATCGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCAGAATTC
CGTCAGATGTGGGCCGAATTTGAATGGGAAAACAAAGTGACAGTTAACACCAACATGGTTGATTTAAATG
ACTACTTACAGCACATATTAAAGTCAACCAATATGAAATGCCTGACTCCAGAAAAGGCCCTTTCTGGTTA
CTGTGGCTTTATGGCAGCCAACCTTTATGCTCGTTCCATATTTGGTGAAGATGCACTTGCAAATGTCAGC
ATTGAGAAGCCAATTCACCAGGGACCAGATGCTGCTGTTACCGGCCATATAAGAATTCGTGCAAAGAGCC
AGGGAATGGCCTTAAGTCTTGGAGATAAAATCAACTTGTCACAGAAGAAAACTAGTATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206009 protein sequence
Red=Cloning site Green=Tags(s)

MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQ
DHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAI
RACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLS
TCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKA
AAQCYIDLIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSS
RNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADV
LEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVE
SEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATT
LTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDI
FNKECRQSLSHMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNT
QRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVE
KPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEF
RQMWAEFEWENKVTVNTNMVDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVS
IEKPIHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016451
ORF Size 2859 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016451.4
RefSeq Size 3490 bp
RefSeq ORF 2862 bp
Locus ID 1315
UniProt ID P53618
Cytogenetics 11p15.2
Domains Adaptin_N
MW 107.1 kDa
Gene Summary This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

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