ADAMTS1 (NM_006988) Human Tagged ORF Clone

CAT#: RC205984

ADAMTS1 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 1 (ADAMTS1)

Product Images

Circular map for RC205984

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ADAMTS1 (Cat# RC205984, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS1(Cat# TA804585). Positive lysates LY416271 (100ug) and LC416271 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416271) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205984 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADAMTS1
• Synonyms: C3-C5; METH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205984 representing NM_006988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGAGCTGTGCCCGAGGGGTTCGGAAGGCGCAAGCTGGGCAGCGACATGGGGAACGCGGAGCGGG
  CTCCGGGGTCTCGGAGCTTTGGGCCCGTACCCACGCTGCTGCTGCTCGCCGCGGCGCTACTGGCCGTGTC
  GGACGCACTCGGGCGCCCCTCCGAGGAGGACGAGGAGCTAGTGGTGCCGGAGCTGGAGCGCGCCCCGGGA
  CACGGGACCACGCGCCTCCGCCTGCACGCCTTTGACCAGCAGCTGGATCTGGAGCTGCGGCCCGACAGCA
  GCTTTTTGGCGCCCGGCTTCACGCTCCAGAACGTGGGGCGCAAATCCGGGTCCGAGACGCCGCTTCCGGA
  AACCGACCTGGCGCACTGCTTCTACTCCGGCACCGTGAATGGCGATCCCAGCTCGGCTGCCGCCCTCAGC
  CTCTGCGAGGGCGTGCGCGGCGCCTTCTACCTGCTGGGGGAGGCGTATTTCATCCAGCCGCTGCCCGCCG
  CCAGCGAGCGCCTCGCCACCGCCGCCCCAGGGGAGAAGCCGCCGGCACCACTACAGTTCCACCTCCTGCG
  GCGGAATCGGCAGGGCGACGTAGGCGGCACGTGCGGGGTCGTGGACGACGAGCCCCGGCCGACTGGGAAA
  GCGGAGACCGAAGACGAGGACGAAGGGACTGAGGGCGAGGACGAAGGGCCTCAGTGGTCGCCGCAGGACC
  CGGCACTGCAAGGCGTAGGACAGCCCACAGGAACTGGAAGCATAAGAAAGAAGCGATTTGTGTCCAGTCA
  CCGCTATGTGGAAACCATGCTTGTGGCAGACCAGTCGATGGCAGAATTCCACGGCAGTGGTCTAAAGCAT
  TACCTTCTCACGTTGTTTTCGGTGGCAGCCAGATTGTACAAACACCCCAGCATTCGTAATTCAGTTAGCC
  TGGTGGTGGTGAAGATCTTGGTCATCCACGATGAACAGAAGGGGCCGGAAGTGACCTCCAATGCTGCCCT
  CACTCTGCGGAACTTTTGCAACTGGCAGAAGCAGCACAACCCACCCAGTGACCGGGATGCAGAGCACTAT
  GACACAGCAATTCTTTTCACCAGACAGGACTTGTGTGGGTCCCAGACATGTGATACTCTTGGGATGGCTG
  ATGTTGGAACTGTGTGTGATCCGAGCAGAAGCTGCTCCGTCATAGAAGATGATGGTTTACAAGCTGCCTT
  CACCACAGCCCATGAATTAGGCCACGTGTTTAACATGCCACATGATGATGCAAAGCAGTGTGCCAGCCTT
  AATGGTGTGAACCAGGATTCCCACATGATGGCGTCAATGCTTTCCAACCTGGACCACAGCCAGCCTTGGT
  CTCCTTGCAGTGCCTACATGATTACATCATTTCTGGATAATGGTCATGGGGAATGTTTGATGGACAAGCC
  TCAGAATCCCATACAGCTCCCAGGCGATCTCCCTGGCACCTCGTACGATGCCAACCGGCAGTGCCAGTTT
  ACATTTGGGGAGGACTCCAAACACTGCCCTGATGCAGCCAGCACATGTAGCACCTTGTGGTGTACCGGCA
  CCTCTGGTGGGGTGCTGGTGTGTCAAACCAAACACTTCCCGTGGGCGGATGGCACCAGCTGTGGAGAAGG
  GAAATGGTGTATCAACGGCAAGTGTGTGAACAAAACCGACAGAAAGCATTTTGATACGCCTTTTCATGGA
  AGCTGGGGAATGTGGGGGCCTTGGGGAGACTGTTCGAGAACGTGCGGTGGAGGAGTCCAGTACACGATGA
  GGGAATGTGACAACCCAGTCCCAAAGAATGGAGGGAAGTACTGTGAAGGCAAACGAGTGCGCTACAGATC
  CTGTAACCTTGAGGACTGTCCAGACAATAATGGAAAAACCTTTAGAGAGGAACAATGTGAAGCACACAAC
  GAGTTTTCAAAAGCTTCCTTTGGGAGTGGGCCTGCGGTGGAATGGATTCCCAAGTACGCTGGCGTCTCAC
  CAAAGGACAGGTGCAAGCTCATCTGCCAAGCCAAAGGCATTGGCTACTTCTTCGTTTTGCAGCCCAAGGT
  TGTAGATGGTACTCCATGTAGCACAGATTCCACCTCTGTCTGTGTGCAAGGACAGTGTGTAAAAGCTGGT
  TGTGATCGCATCATAGACTCCAAAAAGAAGTTTGATAAATGTGGTGTTTGCGGGGGAAATGGATCTACTT
  GTAAAAAAATATCAGGATCAGTTACTAGTGCAAAACCTGGATATCATGATATCATCACAATTCCAACTGG
  AGCCACCAACATCGAAGTGAAACAGCGGAACCAGAGGGGATCCAGGAACAATGGCAGCTTTCTTGCCATC
  AAAGCTGCTGATGGCACATATATTCTTAATGGTGACTACACTTTGTCCACCTTAGAGCAAGACATTATGT
  ACAAAGGTGTTGTCTTGAGGTACAGCGGCTCCTCTGCGGCATTGGAAAGAATTCGCAGCTTTAGCCCTCT
  CAAAGAGCCCTTGACCATCCAGGTTCTTACTGTGGGCAATGCCCTTCGACCTAAAATTAAATACACCTAC
  TTCGTAAAGAAGAAGAAGGAATCTTTCAATGCTATCCCCACTTTTTCAGCATGGGTCATTGAAGAGTGGG
  GCGAATGTTCTAAGTCATGTGAATTGGGTTGGCAGAGAAGACTGGTAGAATGCCGAGACATTAATGGACA
  GCCTGCTTCCGAGTGTGCAAAGGAAGTGAAGCCAGCCAGCACCAGACCTTGTGCAGACCATCCCTGCCCC
  CAGTGGCAGCTGGGGGAGTGGTCATCATGTTCTAAGACCTGTGGGAAGGGTTACAAAAAAAGAAGCTTGA
  AGTGTCTGTCCCATGATGGAGGGGTGTTATCTCATGAGAGCTGTGATCCTTTAAAGAAACCTAAACATTT
  CATAGACTTTTGCACAATGGCAGAATGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205984 representing NM_006988
  Red=Cloning site Green=Tags(s)
  
  MQRAVPEGFGRRKLGSDMGNAERAPGSRSFGPVPTLLLLAAALLAVSDALGRPSEEDEELVVPELERAPG
  HGTTRLRLHAFDQQLDLELRPDSSFLAPGFTLQNVGRKSGSETPLPETDLAHCFYSGTVNGDPSSAAALS
  LCEGVRGAFYLLGEAYFIQPLPAASERLATAAPGEKPPAPLQFHLLRRNRQGDVGGTCGVVDDEPRPTGK
  AETEDEDEGTEGEDEGPQWSPQDPALQGVGQPTGTGSIRKKRFVSSHRYVETMLVADQSMAEFHGSGLKH
  YLLTLFSVAARLYKHPSIRNSVSLVVVKILVIHDEQKGPEVTSNAALTLRNFCNWQKQHNPPSDRDAEHY
  DTAILFTRQDLCGSQTCDTLGMADVGTVCDPSRSCSVIEDDGLQAAFTTAHELGHVFNMPHDDAKQCASL
  NGVNQDSHMMASMLSNLDHSQPWSPCSAYMITSFLDNGHGECLMDKPQNPIQLPGDLPGTSYDANRQCQF
  TFGEDSKHCPDAASTCSTLWCTGTSGGVLVCQTKHFPWADGTSCGEGKWCINGKCVNKTDRKHFDTPFHG
  SWGMWGPWGDCSRTCGGGVQYTMRECDNPVPKNGGKYCEGKRVRYRSCNLEDCPDNNGKTFREEQCEAHN
  EFSKASFGSGPAVEWIPKYAGVSPKDRCKLICQAKGIGYFFVLQPKVVDGTPCSTDSTSVCVQGQCVKAG
  CDRIIDSKKKFDKCGVCGGNGSTCKKISGSVTSAKPGYHDIITIPTGATNIEVKQRNQRGSRNNGSFLAI
  KAADGTYILNGDYTLSTLEQDIMYKGVVLRYSGSSAALERIRSFSPLKEPLTIQVLTVGNALRPKIKYTY
  FVKKKKESFNAIPTFSAWVIEEWGECSKSCELGWQRRLVECRDINGQPASECAKEVKPASTRPCADHPCP
  QWQLGEWSSCSKTCGKGYKKRSLKCLSHDGGVLSHESCDPLKKPKHFIDFCTMAECS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006988
• ORF Size: 2901 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006988.5
• RefSeq Size: 4670 bp
• RefSeq ORF: 2904 bp
• Locus ID: 9510
• UniProt ID: Q9UHI8
• Cytogenetics: 21q21.3
• Domains: tsp_1, Reprolysin, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 105.39 kDa
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]