Neurofilament (NEFL) (NM_006158) Human Tagged ORF Clone

CAT#: RC205920

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NEFL (Myc-DDK-tagged)-Human neurofilament, light polypeptide (NEFL)



  "NM_006158" in other vectors (6)

Reconstitution Protocol

USD 758.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NEFL
Synonyms CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205920 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTCCTTCAGCTACGAGCCGTACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGG
TGCACATCTCCAGCGTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGT
GTCTTCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTGGAGAAC
CTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACGCAGGAGAAGGCGCAGC
TCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAACAAGGT
CCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAGCACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAG
CAGGAGATCCGCGACCTGCGCCTGGCGGCGGAAGATGCCACCAACGAGAAGCAGGCGCTCCAGGGCGAGC
GCGAAGGGCTGGAGGAGACCCTGCGCAACCTGCAGGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGA
CGCCGAGGGCCGGCTGATGGAAGCGCGCAAAGGCGCCGACGAGGCGGCGCTCGCTCGCGCCGAGCTCGAG
AAGCGCATCGACAGCTTGATGGACGAAATCTCTTTTCTGAAGAAAGTGCACGAAGAGGAGATCGCCGAAC
TGCAGGCGCAGATCCAGTACGCGCAGATCTCCGTGGAGATGGACGTGACCAAGCCCGACCTTTCCGCCGC
GCTCAAGGACATCCGCGCGCAGTACGAGAAGCTGGCCGCCAAGAACATGCAGAACGCTGAGGAATGGTTC
AAGAGCCGCTTCACCGTGCTGACCGAGAGCGCCGCCAAGAACACCGACGCCGTGCGCGCCGCCAAGGACG
AGGTGTCCGAGAGCCGTCGTCTGCTCAAGGCCAAGACCCTGGAAATCGAAGCATGCCGGGGCATGAATGA
AGCGCTGGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAACGCCGACATCAGCGCTATGCAGGACACG
ATCAACAAATTAGAAAATGAATTGAGGACCACAAAGAGTGAAATGGCACGATACCTAAAAGAATACCAAG
ACCTCCTCAACGTGAAGATGGCTTTGGATATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGAGGA
GACCCGACTCAGTTTCACCAGCGTGGGAAGCATAACCAGTGGCTACTCCCAGAGCTCCCAGGTCTTTGGC
CGATCTGCCTACGGCGGTTTACAGACCAGCTCCTATCTGATGTCCACCCGCTCCTTCCCGTCCTACTACA
CCAGCCATGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTGCCAAGGCTGAGGAAGCCAA
GGATGAGCCCCCCTCTGAAGGAGAAGCCGAGGAGGAGGAGAAGGACAAGGAAGAGGCCGAGGAAGAGGAG
GCAGCTGAAGAGGAAGAAGCTGCCAAGGAAGAGTCTGAAGAAGCAAAAGAAGAAGAAGAAGGAGGTGAAG
GTGAAGAAGGAGAGGAAACCAAAGAAGCTGAAGAGGAGGAGAAGAAAGTTGAAGGTGCTGGGGAGGAACA
AGCAGCTAAGAAGAAAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205920 protein sequence
Red=Cloning site Green=Tags(s)

MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLEN
LDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYE
QEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELE
KRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWF
KSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDT
INKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFG
RSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEE
AAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006158
ORF Size 1629 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006158.5
RefSeq Size 3854 bp
RefSeq ORF 1632 bp
Locus ID 4747
UniProt ID P07196
Cytogenetics 8p21.2
Domains filament, filament_head
Protein Families Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways Amyotrophic lateral sclerosis (ALS)
MW 61.5 kDa
Gene Summary Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

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