PSMA3 (NM_002788) Human Tagged ORF Clone
Lenti ORF clone of Human proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 1, Myc-DDK-tagged
|Product Name||PSMA3 (NM_002788) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-RsrII Cloning Scheme for this gene|
|ORF Size||765 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1014 bp|
|RefSeq ORF||768 bp|
|Protein Families||Druggable Genome, Protease, Stem cell - Pluripotency|
|Gene Summary||The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|SC105889||PSMA3 (untagged)-Human proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 1||
|RC205917||PSMA3 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 1||
|RG205917||PSMA3 (GFP-tagged) - Human proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 1||
|RC205917L4||Lenti ORF clone of Human proteasome (prosome, macropain) subunit, alpha type, 3 (PSMA3), transcript variant 1, mGFP tagged||