DHX15 (NM_001358) Human Tagged ORF Clone

CAT#: RC205914

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DHX15 (Myc-DDK-tagged)-Human DEAH (Asp-Glu-Ala-His) box polypeptide 15 (DHX15)



  "NM_001358" in other vectors (6)

Reconstitution Protocol

USD 728.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DHX15
Synonyms DBP1; DDX15; PRP43; PRPF43; PrPp43p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAAGCGGCACCGGTTGGACCTAGGGGAGGATTACCCCTCTGGCAAGAAGCGTGCGGGGACCGATG
GGAAGGATCGAGATCGAGACCGGGATCGTGAAGATCGGTCTAAAGATCGAGACCGAGAACGTGATAGAGG
AGATAGAGAGCGAGAGAGGGAGAAAGAAAAGGAGAAGGAGTTGCGAGCTTCAACAAATGCTATGCTTATC
AGTGCTGGATTACCACCTTTGAAAGCTTCCCATTCAGCTCACTCAACCCACTCAGCACATTCAACGCATT
CAACACATTCTGCTCATTCAACGCATGCCGGACATGCAGGTCACACGTCACTTCCACAGTGCATTAATCC
GTTCACCAACTTACCCCATACTCCTCGATACTATGATATTCTAAAGAAACGTCTTCAGCTCCCTGTTTGG
GAATACAAGGATAGGTTTACAGATATTCTGGTTAGACATCAGTCCTTTGTACTGGTTGGTGAGACTGGGT
CTGGTAAAACAACACAGATTCCACAGTGGTGTGTGGAGTACATGCGATCATTACCAGGACCCAAGAGAGG
AGTTGCCTGTACCCAACCCAGGAGAGTGGCTGCAATGAGTGTGGCTCAGAGAGTTGCTGATGAGATGGAT
GTGATGTTGGGCCAGGAAGTTGGTTACTCCATTCGATTTGAAGACTGCAGTAGTGCAAAAACCATTCTTA
AGTATATGACTGATGGGATGTTACTTCGTGAAGCTATGAATGATCCCCTCCTGGAGCGTTATGGTGTAAT
AATTCTTGATGAGGCTCATGAGAGGACACTGGCTACAGATATTCTAATGGGTGTTCTGAAGGAAGTTGTA
AGACAGAGATCAGATTTAAAGGTTATAGTTATGAGCGCTACTCTAGATGCAGGAAAATTCCAGATTTACT
TTGATAACTGTCCTCTCCTAACTATTCCTGGGCGTACACATCCTGTTGAGATCTTCTATACTCCAGAACC
AGAGAGAGATTATCTTGAAGCAGCAATTCGAACAGTTATCCAGATTCATATGTGTGAAGAGGAAGAGGGA
GATCTTCTTCTTTTCTTAACTGGTCAAGAGGAAATTGATGAAGCCTGTAAGAGAATAAAGCGTGAAGTTG
ATGATTTGGGCCCTGAAGTTGGTGACATTAAAATCATTCCATTGTATTCTACACTTCCACCTCAGCAGCA
GCAACGCATTTTTGAGCCTCCACCTCCCAAAAAACAGAATGGAGCAATTGGAAGAAAGGTAGTTGTGTCA
ACTAACATAGCAGAGACGTCTTTGACAATAGATGGTGTGGTGTTTGTGATTGATCCTGGATTTGCGAAAC
AGAAGGTCTACAATCCTCGAATCAGAGTTGAGTCCCTTTTGGTGACAGCTATTAGTAAAGCTTCAGCTCA
GCAAAGGGCTGGTCGAGCTGGACGTACCAGACCTGGAAAATGCTTCAGACTTTACACAGAGAAAGCTTAT
AAAACAGAAATGCAGGATAACACCTATCCTGAGATTTTGCGTTCTAATTTAGGATCAGTTGTGTTACAAT
TGAAGAAACTTGGTATTGATGACTTGGTACATTTTGATTTTATGGATCCACCAGCTCCTGAAACTCTGAT
GAGAGCCCTGGAACTTTTGAATTACCTGGCTGCTTTAAATGATGATGGAGATCTGACTGAATTGGGATCC
ATGATGGCAGAGTTTCCTCTAGATCCACAGCTCGCAAAAATGGTTATTGCAAGTTGTGACTACAACTGTT
CTAATGAGGTCCTATCTATTACTGCTATGTTGTCAGTCCCACAGTGTTTTGTTCGCCCCACGGAGGCCAA
GAAAGCCGCAGATGAGGCCAAGATGAGATTTGCCCACATAGATGGAGATCATCTGACACTGCTGAACGTC
TACCATGCTTTTAAACAAAATCATGAATCGGTTCAGTGGTGTTATGACAACTTCATTAACTACAGGTCCC
TGATGTCCGCAGACAATGTACGCCAGCAGCTATCTCGAATTATGGACAGATTTAATTTGCCTCGTCGAAG
TACTGACTTTACAAGCAGGGACTATTATATTAATATAAGAAAAGCTTTGGTTACTGGGTATTTTATGCAG
GTGGCACATTTAGAACGAACAGGGCATTACTTAACTGTGAAAGATAACCAGGTGGTTCAGTTGCATCCCT
CTACTGTTCTTGACCACAAACCTGAATGGGTGCTTTATAATGAGTTTGTTCTAACAACAAAGAATTACAT
CCGGACATGTACAGATATCAAGCCAGAATGGTTGGTGAAAATTGCCCCTCAATATTATGACATGAGCAAT
TTCCCACAGTGTGAAGCAAAGAGACAGTTGGACCGCATCATTGCCAAACTTCAATCCAAGGAATATTCAC
AGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205914 protein sequence
Red=Cloning site Green=Tags(s)

MSKRHRLDLGEDYPSGKKRAGTDGKDRDRDRDREDRSKDRDRERDRGDREREREKEKEKELRASTNAMLI
SAGLPPLKASHSAHSTHSAHSTHSTHSAHSTHAGHAGHTSLPQCINPFTNLPHTPRYYDILKKRLQLPVW
EYKDRFTDILVRHQSFVLVGETGSGKTTQIPQWCVEYMRSLPGPKRGVACTQPRRVAAMSVAQRVADEMD
VMLGQEVGYSIRFEDCSSAKTILKYMTDGMLLREAMNDPLLERYGVIILDEAHERTLATDILMGVLKEVV
RQRSDLKVIVMSATLDAGKFQIYFDNCPLLTIPGRTHPVEIFYTPEPERDYLEAAIRTVIQIHMCEEEEG
DLLLFLTGQEEIDEACKRIKREVDDLGPEVGDIKIIPLYSTLPPQQQQRIFEPPPPKKQNGAIGRKVVVS
TNIAETSLTIDGVVFVIDPGFAKQKVYNPRIRVESLLVTAISKASAQQRAGRAGRTRPGKCFRLYTEKAY
KTEMQDNTYPEILRSNLGSVVLQLKKLGIDDLVHFDFMDPPAPETLMRALELLNYLAALNDDGDLTELGS
MMAEFPLDPQLAKMVIASCDYNCSNEVLSITAMLSVPQCFVRPTEAKKAADEAKMRFAHIDGDHLTLLNV
YHAFKQNHESVQWCYDNFINYRSLMSADNVRQQLSRIMDRFNLPRRSTDFTSRDYYINIRKALVTGYFMQ
VAHLERTGHYLTVKDNQVVQLHPSTVLDHKPEWVLYNEFVLTTKNYIRTCTDIKPEWLVKIAPQYYDMSN
FPQCEAKRQLDRIIAKLQSKEYSQY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001358
ORF Size 2385 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001358.3
RefSeq Size 3019 bp
RefSeq ORF 2388 bp
Locus ID 1665
UniProt ID O43143
Cytogenetics 4p15.2
Domains DEAD, helicase_C, HA2
Protein Pathways Spliceosome
MW 90.9 kDa
Gene Summary The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing. [provided by RefSeq, Jul 2008]

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