Aromatase (CYP19A1) (NM_000103) Human Tagged ORF Clone

CAT#: RC205890

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CYP19A1 (Myc-DDK-tagged)-Human cytochrome P450, family 19, subfamily A, polypeptide 1 (CYP19A1), transcript variant 1



  "NM_000103" in other vectors (6)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Aromatase (CYP19A1) (NM_000103) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CYP19A1
Synonyms ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC205890 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTTGGAAATGCTGAACCCGATACATTATAACATCACCAGCATCGTGCCTGAAGCCATGCCTGCTG
CCACCATGCCAGTCCTGCTCCTCACTGGCCTTTTTCTCTTGGTGTGGAATTATGAGGGCACATCCTCAAT
ACCAGGTCCTGGCTACTGCATGGGAATTGGACCCCTCATCTCCCACGGCAGATTCCTGTGGATGGGGATC
GGCAGTGCCTGCAACTACTACAACCGGGTGTATGGAGAATTCATGCGAGTCTGGATCTCTGGAGAGGAAA
CACTCATTATCAGCAAGTCCTCAAGTATGTTCCACATAATGAAGCACAATCATTACAGCTCTCGATTCGG
CAGCAAACTTGGGCTGCAGTGCATCGGTATGCATGAGAAAGGCATCATATTTAACAACAATCCAGAGCTC
TGGAAAACAACTCGACCCTTCTTTATGAAAGCTCTGTCAGGCCCCGGCCTTGTTCGTATGGTCACAGTCT
GTGCTGAATCCCTCAAAACACATCTGGACAGGTTGGAGGAGGTGACCAATGAATCGGGCTATGTGGACGT
GTTGACCCTTCTGCGTCGTGTCATGCTGGACACCTCTAACACGCTCTTCTTGAGGATCTCTTTGGACGAA
AGTGCTATCGTGGTTAAAATCCAAGGTTATTTTGATGCATGGCAAGCTCTCCTCATCAAACCAGACATCT
TCTTTAAGATTTCTTGGCTATACAAAAAGTATGAGAAGTCTGTCAAGGATTTGAAAGATGCCATAGAAGT
TCTGATAGCAGAAAAAAGACGCAGGATTTCCACAGAAGAGAAACTGGAAGAATGTATGGACTTTGCCACT
GAGTTGATTTTAGCAGAGAAACGTGGTGACCTGACAAGAGAGAATGTGAACCAGTGCATATTGGAAATGC
TGATCGCAGCTCCTGACACCATGTCTGTCTCTTTGTTCTTCATGCTATTTCTCATTGCAAAGCACCCTAA
TGTTGAAGAGGCAATAATAAAGGAAATCCAGACTGTTATTGGTGAGAGAGACATAAAGATTGATGATATA
CAAAAATTAAAAGTGATGGAAAACTTCATTTATGAGAGCATGCGGTACCAGCCTGTCGTGGACTTGGTCA
TGCGCAAAGCCTTAGAAGATGATGTAATCGATGGCTACCCAGTGAAAAAGGGGACAAACATTATCCTGAA
TATTGGAAGGATGCACAGACTCGAGTTTTTCCCCAAACCCAATGAATTTACTCTTGAAAATTTTGCAAAG
AATGTTCCTTATAGGTACTTTCAGCCATTTGGCTTTGGGCCCCGTGGCTGTGCAGGAAAGTACATCGCCA
TGGTGATGATGAAAGCCATCCTCGTTACACTTCTGAGACGATTCCACGTGAAGACATTGCAAGGACAGTG
TGTTGAGAGCATACAGAAGATACACGACTTGTCCTTGCACCCAGATGAGACTAAAAACATGCTGGAAATG
ATCTTTACCCCAAGAAACTCAGACAGGTGTCTGGAACAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205890 protein sequence
Red=Cloning site Green=Tags(s)

MVLEMLNPIHYNITSIVPEAMPAATMPVLLLTGLFLLVWNYEGTSSIPGPGYCMGIGPLISHGRFLWMGI
GSACNYYNRVYGEFMRVWISGEETLIISKSSSMFHIMKHNHYSSRFGSKLGLQCIGMHEKGIIFNNNPEL
WKTTRPFFMKALSGPGLVRMVTVCAESLKTHLDRLEEVTNESGYVDVLTLLRRVMLDTSNTLFLRISLDE
SAIVVKIQGYFDAWQALLIKPDIFFKISWLYKKYEKSVKDLKDAIEVLIAEKRRRISTEEKLEECMDFAT
ELILAEKRGDLTRENVNQCILEMLIAAPDTMSVSLFFMLFLIAKHPNVEEAIIKEIQTVIGERDIKIDDI
QKLKVMENFIYESMRYQPVVDLVMRKALEDDVIDGYPVKKGTNIILNIGRMHRLEFFPKPNEFTLENFAK
NVPYRYFQPFGFGPRGCAGKYIAMVMMKAILVTLLRRFHVKTLQGQCVESIQKIHDLSLHPDETKNMLEM
IFTPRNSDRCLEH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_000103
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000103.1, NM_000103.2, NM_000103.3, NP_000094.2
RefSeq Size 4422 bp
RefSeq ORF 1512 bp
Locus ID 1588
Cytogenetics 15q21.2
Domains p450
Protein Families Druggable Genome, P450
Protein Pathways Androgen and estrogen metabolism, Metabolic pathways
MW 57.9 kDa
Gene Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]

Citations (2)

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