SNAP47 (NM_053052) Human Tagged ORF Clone

CAT#: RC205867

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SNAP47 (Myc-DDK-tagged)-Human synaptosomal-associated protein, 47kDa (SNAP47)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SNAP47
Synonyms C1orf142; ESFI5812; HEL-S-290; HEL170; SNAP-47; SVAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205867 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGCGGCTCGCCGCGGTCTTCACTGCGCAGGCGCCGAGCGGCCGAGGCGCCGCGGTCGGCTCTGGG
ACTCGTCTGGCGTCCCTCAGAGGCAGAAGAGGCCTGGACCTTGGCGCACACAGACCCAGGAACAGATGAG
CAGGGATGTCTGCATCCACACCTGGCCGTGCACCTACTACCTGGAGCCCAAGAGGCGATGGGTTACTGGA
CAGCTGTCCTTAACATCGCTGTCGCTCAGGTTCATGACTGACAGCACTGGAGAGATTCTGGTCAGCTTCC
CCCTCTCCAGCATAGTTGAGATCAAGAAGGAGGCTTCACATTTTATCTTCAGCTCCATCACCATCCTGGA
GAAGGGCCATGCCAAGCACTGGTTCAGCTCCCTGCGGCCAAGTCGAAATGTGGTCTTCAGCATCATCGAG
CATTTCTGGAGGGAGCTGCTGCTGTCTCAGCCTGGAGCCGTGGCAGACGCATCTGTCCCAAGGACCCGGG
GCGAGGAGCTGACGGGACTCATGGCTGGATCCCAGAAACGCCTGGAGGACACGGCGAGGGTCCTGCACCA
CCAGGGCCAGCAGCTGGACAGCGTCATGAGAGGCCTGGACAAGATGGAGTCAGACCTGGAGGTGGCGGAC
AGATTGCTGACAGAACTGGAATCTCCTGCTTGGTGGCCCTTTAGCTCCAAGCTTTGGAAGACACCACCGG
AAACAAAGCCCAGGGAAGATGTCTCCATGACCAGTTGTGAACCCTTTGGGAAAGAAGGGATACTGATAAA
AATTCCTGCTGTTATTTCCCACAGAACAGAGTCTCACGTTAAACCAGGGAGGCTCACCGTCCTTGTGTCT
GGGTTGGAAATACATGACTCCAGTTCTTTGCTCATGCACAGGTTTGAAAGAGAAGACGTGGACGACATCA
AGGTCCACTCACCTTACGAAATTAGCATCCGCCAGCGGTTTATTGGAAAGCCAGACATGGCCTATCGTTT
GATATCTGCCAAGATGCCAGAGGTTATCCCCATTTTAGAAGTGCAGTTCAGCAAGAAGATGGAGCTGTTA
GAAGATGCATTGGTGCTCAGAAGCGCAAGAACCTCTTCCCCCGCAGAGAAGAGCTGCTCAGTCTGGCATG
CAGCATCTGGGCTGATGGGCTGTACCCTGCACCGTGAGCCACCCGCAGGAGACCAGGAGGGCACAGCACT
GCACCTGCAGACAAGCCTGCCAGCCCTTTCTGAGGCAGATACCCAGGAACTAACCCAGATCCTGAGGAGG
ATGAAGGGGCTGGCCCTGGAGGCCGAGAGTGAGCTGGAGAGACAAGACGAAGCCCTGGATGGCGTTGCAG
CAGCTGTGGACAGGGCAACCTTGACCATCGACAAGCACAACAGGCGGATGAAGAGGCTGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205867 protein sequence
Red=Cloning site Green=Tags(s)

MRAARRGLHCAGAERPRRRGRLWDSSGVPQRQKRPGPWRTQTQEQMSRDVCIHTWPCTYYLEPKRRWVTG
QLSLTSLSLRFMTDSTGEILVSFPLSSIVEIKKEASHFIFSSITILEKGHAKHWFSSLRPSRNVVFSIIE
HFWRELLLSQPGAVADASVPRTRGEELTGLMAGSQKRLEDTARVLHHQGQQLDSVMRGLDKMESDLEVAD
RLLTELESPAWWPFSSKLWKTPPETKPREDVSMTSCEPFGKEGILIKIPAVISHRTESHVKPGRLTVLVS
GLEIHDSSSLLMHRFEREDVDDIKVHSPYEISIRQRFIGKPDMAYRLISAKMPEVIPILEVQFSKKMELL
EDALVLRSARTSSPAEKSCSVWHAASGLMGCTLHREPPAGDQEGTALHLQTSLPALSEADTQELTQILRR
MKGLALEAESELERQDEALDGVAAAVDRATLTIDKHNRRMKRLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053052
ORF Size 1392 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053052.3
RefSeq Size 2388 bp
RefSeq ORF 1260 bp
Locus ID 116841
UniProt ID Q5SQN1
Cytogenetics 1q42.13
Protein Pathways SNARE interactions in vesicular transport
MW 52.5 kDa
Gene Summary Plays a role in intracellular membrane fusion.[UniProtKB/Swiss-Prot Function]
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