SNAP47 (NM_053052) Human Tagged ORF Clone

CAT#: RC205867

SNAP47 (Myc-DDK-tagged)-Human synaptosomal-associated protein, 47kDa (SNAP47)

Product Images

Western blot validation of overexpression lysate (Cat# LY409312) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205867 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNAP47
• Synonyms: C1orf142; ESFI5812; HEL-S-290; HEL170; SNAP-47; SVAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205867 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCGGCTCGCCGCGGTCTTCACTGCGCAGGCGCCGAGCGGCCGAGGCGCCGCGGTCGGCTCTGGG
  ACTCGTCTGGCGTCCCTCAGAGGCAGAAGAGGCCTGGACCTTGGCGCACACAGACCCAGGAACAGATGAG
  CAGGGATGTCTGCATCCACACCTGGCCGTGCACCTACTACCTGGAGCCCAAGAGGCGATGGGTTACTGGA
  CAGCTGTCCTTAACATCGCTGTCGCTCAGGTTCATGACTGACAGCACTGGAGAGATTCTGGTCAGCTTCC
  CCCTCTCCAGCATAGTTGAGATCAAGAAGGAGGCTTCACATTTTATCTTCAGCTCCATCACCATCCTGGA
  GAAGGGCCATGCCAAGCACTGGTTCAGCTCCCTGCGGCCAAGTCGAAATGTGGTCTTCAGCATCATCGAG
  CATTTCTGGAGGGAGCTGCTGCTGTCTCAGCCTGGAGCCGTGGCAGACGCATCTGTCCCAAGGACCCGGG
  GCGAGGAGCTGACGGGACTCATGGCTGGATCCCAGAAACGCCTGGAGGACACGGCGAGGGTCCTGCACCA
  CCAGGGCCAGCAGCTGGACAGCGTCATGAGAGGCCTGGACAAGATGGAGTCAGACCTGGAGGTGGCGGAC
  AGATTGCTGACAGAACTGGAATCTCCTGCTTGGTGGCCCTTTAGCTCCAAGCTTTGGAAGACACCACCGG
  AAACAAAGCCCAGGGAAGATGTCTCCATGACCAGTTGTGAACCCTTTGGGAAAGAAGGGATACTGATAAA
  AATTCCTGCTGTTATTTCCCACAGAACAGAGTCTCACGTTAAACCAGGGAGGCTCACCGTCCTTGTGTCT
  GGGTTGGAAATACATGACTCCAGTTCTTTGCTCATGCACAGGTTTGAAAGAGAAGACGTGGACGACATCA
  AGGTCCACTCACCTTACGAAATTAGCATCCGCCAGCGGTTTATTGGAAAGCCAGACATGGCCTATCGTTT
  GATATCTGCCAAGATGCCAGAGGTTATCCCCATTTTAGAAGTGCAGTTCAGCAAGAAGATGGAGCTGTTA
  GAAGATGCATTGGTGCTCAGAAGCGCAAGAACCTCTTCCCCCGCAGAGAAGAGCTGCTCAGTCTGGCATG
  CAGCATCTGGGCTGATGGGCTGTACCCTGCACCGTGAGCCACCCGCAGGAGACCAGGAGGGCACAGCACT
  GCACCTGCAGACAAGCCTGCCAGCCCTTTCTGAGGCAGATACCCAGGAACTAACCCAGATCCTGAGGAGG
  ATGAAGGGGCTGGCCCTGGAGGCCGAGAGTGAGCTGGAGAGACAAGACGAAGCCCTGGATGGCGTTGCAG
  CAGCTGTGGACAGGGCAACCTTGACCATCGACAAGCACAACAGGCGGATGAAGAGGCTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205867 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRAARRGLHCAGAERPRRRGRLWDSSGVPQRQKRPGPWRTQTQEQMSRDVCIHTWPCTYYLEPKRRWVTG
  QLSLTSLSLRFMTDSTGEILVSFPLSSIVEIKKEASHFIFSSITILEKGHAKHWFSSLRPSRNVVFSIIE
  HFWRELLLSQPGAVADASVPRTRGEELTGLMAGSQKRLEDTARVLHHQGQQLDSVMRGLDKMESDLEVAD
  RLLTELESPAWWPFSSKLWKTPPETKPREDVSMTSCEPFGKEGILIKIPAVISHRTESHVKPGRLTVLVS
  GLEIHDSSSLLMHRFEREDVDDIKVHSPYEISIRQRFIGKPDMAYRLISAKMPEVIPILEVQFSKKMELL
  EDALVLRSARTSSPAEKSCSVWHAASGLMGCTLHREPPAGDQEGTALHLQTSLPALSEADTQELTQILRR
  MKGLALEAESELERQDEALDGVAAAVDRATLTIDKHNRRMKRLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053052
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053052.3
• RefSeq Size: 2388 bp
• RefSeq ORF: 1260 bp
• Locus ID: 116841
• UniProt ID: Q5SQN1
• Cytogenetics: 1q42.13
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 52.5 kDa
• Gene Summary: Plays a role in intracellular membrane fusion.[UniProtKB/Swiss-Prot Function]