FARS2 (NM_006567) Human Tagged ORF Clone

CAT#: RC205843

FARS2 (Myc-DDK-tagged)-Human phenylalanyl-tRNA synthetase 2, mitochondrial (FARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC205843

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  Western blot validation of overexpression lysate (Cat# LY416556) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205843 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FARS2 protein (Cat# TP305843). The protein was produced from HEK293T cells transfected with FARS2 cDNA clone (Cat# RC205843) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FARS2
• Synonyms: COXPD14; FARS1; HSPC320; mtPheRS; PheRS; SPG77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205843 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGCTCAGCTCTCAGGAGAGGTGCCCATGCATATGTCTACCTGGTGAGTAAGGCCAGTCACATCT
  CCAGAGGCCATCAGCACCAGGCCTGGGGATCGAGGCCTCCTGCAGCAGAGTGTGCCACCCAAAGAGCTCC
  AGGCAGTGTGGTGGAGCTGCTGGGCAAATCCTACCCTCAGGACGACCACAGCAACCTCACCCGGAAGGTC
  CTCACCAGAGTTGGCAGGAACCTGCACAACCAGCAGCATCACCCTCTGTGGCTGATCAAGGAGAGGGTGA
  AGGAGCACTTCTACAAGCAGTATGTGGGCCGCTTTGGGACCCCGTTGTTCTCGGTCTACGACAACCTTTC
  TCCAGTGGTCACGACCTGGCAGAACTTTGACAGCCTGCTCATCCCAGCTGATCACCCCAGCAGGAAGAAG
  GGGGACAACTATTACCTGAATCGGACTCACATGCTGAGAGCGCACACGTCTGCACACCAGTGGGACTTGC
  TGCACGCGGGACTGGATGCCTTCCTGGTGGTGGGTGATGTCTACAGGCGTGACCAGATCGACTCCCAGCA
  CTACCCTATTTTCCACCAGCTGGAGGCCGTGCGGCTCTTCTCCAAACATGAGTTATTTGCTGGTATAAAG
  GATGGAGAAAGCCTGCAGCTCTTTGAACAAAGTTCTCGCTCTGCGCATAAACAAGAGACACACACCATGG
  AGGCCGTGAAGCTTGTAGAGTTTGATCTTAAGCAAACGCTTACCAGGCTCATGGCACATCTTTTTGGAGA
  TGAGCTGGAGATAAGATGGGTAGACTGCTACTTCCCTTTTACACATCCTTCCTTTGAGATGGAGATCAAC
  TTTCATGGAGAATGGCTGGAAGTTCTTGGCTGCGGGGTGATGGAACAACAACTGGTCAATTCAGCTGGTG
  CTCAAGACCGAATCGGCTGGGCTTTTGGCCTAGGATTAGAAAGGCTAGCCATGATCCTCTACGACATCCC
  TGATATCCGTCTCTTCTGGTGTGAGGACGAGCGCTTCCTGAAGCAGTTCTGTGTATCCAACATTAATCAG
  AAGGTGAAGTTTCAGCCTCTTAGCAAATATCCGGCTGTGATCAATGATATTTCATTCTGGTTGCCCTCTG
  AGAATTACGCAGAAAATGATTTCTATGACTTAGTCCGAACAATTGGAGGAGACCTGGTGGAAAAGGTTGA
  TCTCATAGACAAGTTTGTACATCCAAAGACGCACAAGACCAGCCACTGCTACCGCATCACGTACCGCCAC
  ATGGAACGGACTCTGTCCCAGAGAGAGGTCAGGCACATCCACCAGGCCTTGCAGGAGGCTGCAGTCCAGC
  TGTTGGGTGTGGAGGGCAGGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205843 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVGSALRRGAHAYVYLVSKASHISRGHQHQAWGSRPPAAECATQRAPGSVVELLGKSYPQDDHSNLTRKV
  LTRVGRNLHNQQHHPLWLIKERVKEHFYKQYVGRFGTPLFSVYDNLSPVVTTWQNFDSLLIPADHPSRKK
  GDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIK
  DGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEIN
  FHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQ
  KVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRH
  MERTLSQREVRHIHQALQEAAVQLLGVEGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006567
• ORF Size: 1353 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006567.5
• RefSeq Size: 1841 bp
• RefSeq ORF: 1356 bp
• Locus ID: 10667
• UniProt ID: O95363
• Cytogenetics: 6p25.1
• Domains: tRNA-synt_2d, FDX-ACB
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 52.4 kDa
• Gene Summary: This gene encodes a protein that transfers phenylalanine to its cognate tRNA. This protein localizes to the mitochondrion and plays a role in mitochondrial protein translation. Mutations in this gene can cause combined oxidative phosphorylation deficiency 14 (Alpers encephalopathy). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]