CPSF73 (CPSF3) (NM_016207) Human Tagged ORF Clone

CAT#: RC205834

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CPSF3 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 3, 73kDa (CPSF3)



  "NM_016207" in other vectors (6)

Reconstitution Protocol

USD 939.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CPSF3
Synonyms CPSF-73; CPSF73
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205834 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCGATTCCTGCTGAGGAGAGCGACCAGCTGCTGATCCGACCCCTTGGAGCTGGGCAAGAAGTAG
GAAGATCATGTATTATTCTCGAGTTCAAAGGAAGAAAAATAATGCTCGACTGTGGGATCCACCCTGGCCT
AGAAGGAATGGATGCTCTTCCTTATATTGATTTAATTGACCCAGCTGAGATTGATCTCCTATTAATTAGT
CATTTCCATTTGGATCACTGTGGAGCTCTGCCCTGGTTTCTACAGAAGACAAGTTTCAAAGGAAGAACAT
TTATGACTCATGCCACAAAAGCTATTTATAGATGGCTTCTTTCTGATTATGTCAAAGTTAGTAACATATC
AGCAGACGACATGCTGTATACCGAGACAGATTTGGAAGAAAGCATGGACAAAATTGAAACTATCAACTTT
CATGAAGTTAAGGAAGTTGCGGGAATCAAGTTTTGGTGTTACCATGCAGGTCACGTCCTAGGAGCCGCCA
TGTTCATGATTGAGATCGCAGGCGTGAAGCTTTTGTACACTGGTGATTTCTCAAGACAAGAAGATAGGCA
CTTAATGGCAGCTGAAATTCCTAATATTAAGCCTGATATTCTTATCATTGAATCTACTTATGGGACCCAT
ATCCATGAGAAACGTGAAGAGCGAGAAGCAAGATTCTGTAACACTGTCCACGATATTGTAAACAGAGGAG
GCAGGGGTCTCATTCCTGTCTTTGCTCTTGGAAGGGCTCAGGAGCTGCTCTTGATTCTAGATGAGTACTG
GCAGAATCACCCAGAACTACATGACATTCCAATATACTATGCATCATCTTTGGCCAAGAAGTGTATGGCA
GTGTACCAGACATATGTAAATGCCATGAATGACAAAATCCGCAAACAGATCAACATCAATAATCCCTTTG
TTTTCAAACACATTAGTAACCTCAAGAGCATGGATCATTTTGATGACATTGGTCCCAGTGTTGTAATGGC
CTCCCCAGGCATGATGCAAAGTGGCTTATCCAGAGAATTATTTGAAAGCTGGTGTACTGATAAGAGGAAT
GGTGTCATTATAGCGGGATACTGTGTAGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGAAGAAA
TCACTACTATGTCTGGACAGAAGTTACCACTGAAAATGTCTGTTGATTACATTTCTTTCTCAGCTCACAC
GGATTACCAGCAAACCAGTGAATTTATTCGTGCTTTGAAACCGCCTCATGTGATTTTAGTCCATGGAGAA
CAGAATGAAATGGCCAGATTGAAAGCAGCACTGATTCGAGAATATGAAGATAACGATGAAGTTCACATAG
AGGTTCATAATCCTCGGAATACAGAAGCAGTGACCTTAAACTTCAGAGGAGAAAAACTAGCCAAGGTTAT
GGGATTTTTAGCAGACAAAAAACCAGAACAAGGCCAGCGGGTCTCAGGAATACTTGTTAAAAGAAACTTT
AATTATCACATACTTTCTCCTTGCGACCTGTCCAATTATACTGACCTGGCCATGAGCACGGTGAAGCAGA
CCCAAGCCATTCCATATACTGGTCCCTTTAATTTGCTCTGTTACCAGCTGCAGAAATTGACAGGTGATGT
GGAAGAATTAGAAATTCAAGAAAAACCTGCTCTGAAAGTGTTCAAAAATATTACTGTAATACAAGAACCA
GGCATGGTGGTATTAGAATGGCTGGCAAACCCTTCTAATGATATGTATGCAGATACAGTAACAACTGTGA
TATTGGAAGTTCAGTCAAATCCCAAAATAAGAAAAGGTGCAGTACAGAAGGTTTCTAAAAAATTAGAAAT
GCACGTTTACAGCAAGAGGTTGGAGATCATGCTCCAGGACATATTTGGAGAAGACTGTGTAAGTGTAAAG
GATGACTCTATTCTTAGCGTCACAGTGGACGGGAAAACTGCCAACCTTAACTTGGAGACACGGACTGTAG
AATGTGAAGAGGGAAGTGAAGACGATGAATCCCTCCGAGAAATGGTGGAGCTGGCTGCACAGAGACTGTA
CGAGGCCCTGACGCCAGTTCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205834 protein sequence
Red=Cloning site Green=Tags(s)

MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLIS
HFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINF
HEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTH
IHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMA
VYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMMQSGLSRELFESWCTDKRN
GVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGE
QNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNF
NYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFNLLCYQLQKLTGDVEELEIQEKPALKVFKNITVIQEP
GMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEIMLQDIFGEDCVSVK
DDSILSVTVDGKTANLNLETRTVECEEGSEDDESLREMVELAAQRLYEALTPVH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016207
ORF Size 2052 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016207.4
RefSeq Size 2401 bp
RefSeq ORF 2055 bp
Locus ID 51692
UniProt ID Q9UKF6
Cytogenetics 2p25.1
Domains lactamase_B
MW 77.5 kDa
Gene Summary This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]

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