Apelin (APLN) (NM_017413) Human Tagged ORF Clone
APLN (Myc-DDK-tagged)-Human apelin (APLN)
|Product Name||Apelin (APLN) (NM_017413) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC205832 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205832 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||231 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_017413.1, NM_017413.2, NM_017413.3, NM_017413.4, NP_059109.3|
|RefSeq Size||3238 bp|
|RefSeq ORF||234 bp|
|Protein Families||Druggable Genome, Secreted Protein|
|Gene Summary||This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016]|
|The use of this cDNA Clones has been cited in the following citations:|
Apelin promotes hepatic fibrosis through ERK signaling in LX-2 cells
,Wang, Y;Song, J;Bian, H;Bo, J;Lv, S;Pan, W;Lv, X;,
Mol. Cell. Biochem. 2019
,PubMed ID 31270645
|cDNA Clone Resources|
|SC114101||APLN (untagged)-Human apelin (APLN)||
|RG205832||APLN (GFP-tagged) - Human apelin (APLN)||
|RC205832L1||Lenti ORF clone of Human apelin (APLN), Myc-DDK-tagged||
|RC205832L2||Lenti ORF clone of Human apelin (APLN), mGFP tagged||
|RC205832L3||Lenti ORF clone of Human apelin (APLN), Myc-DDK-tagged||
|RC205832L4||Lenti ORF clone of Human apelin (APLN), mGFP tagged||