ZC3H10 (NM_032786) Human Tagged ORF Clone

CAT#: RC205804

ZC3H10 (Myc-DDK-tagged)-Human zinc finger CCCH-type containing 10 (ZC3H10)

Product Images

Circular map for RC205804

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  Western blot validation of overexpression lysate (Cat# LY409925) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205804 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZC3H10 protein (Cat# TP305804). The protein was produced from HEK293T cells transfected with ZC3H10 cDNA clone (Cat# RC205804) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZC3H10
• Synonyms: ZC3HDC10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205804 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGACCGGGACAGCTATGCCAACGGTACCGGGAGCAGCGGTGGAGGCCCTGGAGGTGGTGGCAGCG
  AGGAGGCCAGTGGGGCAGGGGTAGGCAGTGGCGGGGCCAGCTCAGATGCCATCTGTAGAGACTTCTTGAG
  GAATGTGTGCAAGCGAGGCAAGCGTTGCCGATATCGCCACCCAGACATGAGCGAGGTGTCCAACTTGGGG
  GTGAGCAAAAACGAGTTCATCTTCTGCCATGACTTCCAGAACAAGGAGTGTAGCCGCCCAAATTGCCGTT
  TCATCCATGGCTCCAAGGAGGATGAGGATGGCTATAAGAAGACAGGAGAGCTTCCCCCACGGCTGAGGCA
  GAAAGTAGCAGCTGGCCTTGGCCTTTCACCGGCTGACCTACCAAATGGCAAGGAGGAGGTCCCTATCTGC
  CGTGACTTTCTCAAGGGTGACTGTCAGAGAGGAGCCAAGTGCAAGTTCCGTCACCTGCAACGGGATTTTG
  AGTTTGATGCTCGGGGTGGAGGAGGCACTGGTGGGGGCTCAACAGGCTCAGTCCTCCCAGGACGACGTCA
  TGATCTCTATGATATCTATGACCTTCCTGACAGGGGCTTTGAGGACCATGAGCCAGGCCCAAAACGCCGG
  CGAGGTGGATGCTGCCCCCCTGATGGCCCTCATTTTGAGTCATATGAATATAGTTTGGCTCCACCGCGAG
  GGGTGGAGTGCAGACTGCTAGAGGAGGAGAATGCCATGCTCAGGAAGCGGGTAGAGGAGTTAAAGAAGCA
  GGTCAGCAACCTGCTGGCCACCAATGAGGTACTACTGGAACAAAATGCTCAGTTCCGCAATCAGGCCAAG
  GTCATAACCCTGAGCTCCACTGCACCAGCGACTGAGCAGACTCTGGCCCCCACTGTGGGCACTGTTGCCA
  CTTTTAACCATGGCATTGCCCAGACTCACACTACTCTCAGCAGCCAGGCTCTACAGCCTCGTCCAGTGTC
  CCAGCAAGAACTGGTGGCCCCTGCTGGAGCTCCAGCTGCTCCCCCAACTAATGCTGCACCTCCTGCTGCT
  CCACCACCCCCACCCCCACACTTGACCCCAGAGATCACGCCACTGTCAGCTGCCCTGGCTCAAACAATTG
  CCCAGGGAATGGCACCTCCACCTGTCTCCATGGCTCCTGTGGCTGTATCTGTGGCTCCTGTGGCCCCTGT
  GGCTGTATCGATGGCCCAACCCTTGGCAGGAATCACAATGAGCCACACCACCACTCCCATGGTGACTTAC
  CCTATCGCTTCCCAGAGCATGCGCATCACGGCCATGCCACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205804 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPDRDSYANGTGSSGGGPGGGGSEEASGAGVGSGGASSDAICRDFLRNVCKRGKRCRYRHPDMSEVSNLG
  VSKNEFIFCHDFQNKECSRPNCRFIHGSKEDEDGYKKTGELPPRLRQKVAAGLGLSPADLPNGKEEVPIC
  RDFLKGDCQRGAKCKFRHLQRDFEFDARGGGGTGGGSTGSVLPGRRHDLYDIYDLPDRGFEDHEPGPKRR
  RGGCCPPDGPHFESYEYSLAPPRGVECRLLEEENAMLRKRVEELKKQVSNLLATNEVLLEQNAQFRNQAK
  VITLSSTAPATEQTLAPTVGTVATFNHGIAQTHTTLSSQALQPRPVSQQELVAPAGAPAAPPTNAAPPAA
  PPPPPPHLTPEITPLSAALAQTIAQGMAPPPVSMAPVAVSVAPVAPVAVSMAQPLAGITMSHTTTPMVTY
  PIASQSMRITAMPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032786
• ORF Size: 1302 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032786.3
• RefSeq Size: 2121 bp
• RefSeq ORF: 1305 bp
• Locus ID: 84872
• UniProt ID: Q96K80
• Cytogenetics: 12q13.2
• Domains: zf-CCCH
• MW: 46.1 kDa
• Gene Summary: Specific regulator of miRNA biogenesis. Binds, via the C3H1-type zinc finger domains, to the binding motif 5'-GCAGCGC-3' on microRNA pri-MIR143 and negatively regulates the processing to mature microRNA.[UniProtKB/Swiss-Prot Function]