PPP1R14A (NM_033256) Human Tagged ORF Clone
PPP1R14A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A)
|Type||Human Tagged ORF Clone|
|Synonyms||CPI-17; CPI17; PPP1INL|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC205799 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205799 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||441 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||782 bp|
|RefSeq ORF||444 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Vascular smooth muscle contraction|
|Gene Summary||The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]|
|cDNA Clone Resources|
|RC205799L1||Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A), Myc-DDK-tagged||
|RC205799L2||Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A), mGFP tagged||
|RC205799L3||Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A), Myc-DDK-tagged||
|RC205799L4||Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A), mGFP tagged||
|RG205799||PPP1R14A (GFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A)||
|SC319588||PPP1R14A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 14A (PPP1R14A)||