GABPB2 (NM_144618) Human Tagged ORF Clone

CAT#: RC205763

GABPB2 (Myc-DDK-tagged)-Human GA binding protein transcription factor, beta subunit 2 (GABPB2)

Product Images

Circular map for RC205763

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  Western blot validation of overexpression lysate (Cat# LY408253) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205763 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GABPB2 protein (Cat# TP305763). The protein was produced from HEK293T cells transfected with GABPB2 cDNA clone (Cat# RC205763) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABPB2
• Synonyms: GABPB-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205763 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTGGTGGACTTGGGAAAGAGGTTGCTAGAAGCAGCAAGAAAAGGCCAAGATGATGAAGTGAGAA
  CGTTGATGGCAAATGGCGCCCCATTCACCACAGACTGGCTTGGAACATCACCCCTCCACCTTGCAGCTCA
  ATATGGTCATTATTCCACAGCAGAAGTACTCCTTCGAGCAGGTGTTAGCAGGGATGCCCGGACTAAAGTA
  GACAGGACCCCCTTGCACATGGCTGCAGCCGATGGACATGCGCACATCGTGGAACTGCTTGTTCGGAATG
  GTGCAGATGTGAATGCCAAGGACATGCTGAAGATGACAGCTTTGCATTGGGCCACAGAGCGCCACCATCG
  AGATGTCGTAGAGTTACTTATCAAATATGGAGCTGATGTCCATGCTTTCAGCAAATTTGATAAATCAGCC
  TTTGACATAGCTCTGGAGAAAAACAATGCTGAGATTTTGGTCATCCTCCAGGAAGCAATGCAGAATCAGG
  TGAATGTTAATCCAGAGAGAGCCAACCCTGTGACTGACCCTGTGAGTATGGCTGCTCCATTCATCTTCAC
  GTCGGGTGAGGTTGTTAACCTCGCAAGCCTTATTTCTTCAACCAACACCAAAACAACCTCAGGTGACCCC
  CATGCCTCAACAGTACAGTTTTCAAATTCTACCACCTCAGTGCTGGCTACCCTTGCAGCTCTTGCTGAGG
  CATCAGTCCCCCTCTCCAACTCACACAGAGCCACAGCCAATACAGAGGAAATTATAGAAGGAAATTCCGT
  TGACTCATCAATCCAGCAAGTAATGGGGAGTGGAGGCCAGAGGGTCATCACCATAGTGACTGATGGAGTC
  CCTCTGGGTAATATCCAAACTTCAATCCCTACTGGAGGCATTGGCCAGCCATTTATTGTAACTGTGCAAG
  ATGGACAGCAAGTTCTAACTGTACCTGCTGGTAAGGTTGCAGAGGAGACTGTAATTAAAGAGGAAGAAGA
  AGAGAAGTTGCCACTAACAAAGAAACCAAGGATAGGAGAGAAGACAAACAGTGTGGAGGAAAGCAAGGAA
  GGCAATGAAAGAGAGCTACTACAGCAACAACTCCAGGAGGCCAATCGAAGAGCCCAGGAATACCGACACC
  AGCTCCTAAAGAAAGAGCAGGAAGCAGAACAGTACCGTCTTAAGCTGGAGGCCATAGCCCGACAGCAGCC
  CAATGGAGTTGATTTCACCATGGTTGAAGAGGTGGCTGAGGTAGATGCTGTAGTAGTCACAGAGGGGGAG
  TTGGAAGAGAGAGAGACAAAAGTGACTGGGTCAGCAGGGACCACAGAGCCTCACACTAGAGTTTCCATGG
  CAACTGTTTCATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205763 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLVDLGKRLLEAARKGQDDEVRTLMANGAPFTTDWLGTSPLHLAAQYGHYSTAEVLLRAGVSRDARTKV
  DRTPLHMAAADGHAHIVELLVRNGADVNAKDMLKMTALHWATERHHRDVVELLIKYGADVHAFSKFDKSA
  FDIALEKNNAEILVILQEAMQNQVNVNPERANPVTDPVSMAAPFIFTSGEVVNLASLISSTNTKTTSGDP
  HASTVQFSNSTTSVLATLAALAEASVPLSNSHRATANTEEIIEGNSVDSSIQQVMGSGGQRVITIVTDGV
  PLGNIQTSIPTGGIGQPFIVTVQDGQQVLTVPAGKVAEETVIKEEEEEKLPLTKKPRIGEKTNSVEESKE
  GNERELLQQQLQEANRRAQEYRHQLLKKEQEAEQYRLKLEAIARQQPNGVDFTMVEEVAEVDAVVVTEGE
  LEERETKVTGSAGTTEPHTRVSMATVSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144618
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144618.3
• RefSeq Size: 1953 bp
• RefSeq ORF: 1347 bp
• Locus ID: 126626
• UniProt ID: Q8TAK5
• Cytogenetics: 1q21.3
• Protein Families: Transcription Factors
• MW: 48.6 kDa
• Gene Summary: May function as transcription factor capable of interacting with purine rich repeats (GA repeats).[UniProtKB/Swiss-Prot Function]