MARK3 (NM_002376) Human Tagged ORF Clone

CAT#: RC205758

MARK3 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 3

Product Images

Circular map for RC205758

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MARK3 (Cat# RC205758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3(Cat# TA805790). Positive lysates LY400851 (100ug) and LC400851 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY400851) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205758 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MARK3 protein (Cat# TP305758). The protein was produced from HEK293T cells transfected with MARK3 cDNA clone (Cat# RC205758) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MARK3
• Synonyms: CTAK1; KP78; Par-1a; PAR1A; VIPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205758 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCACATGGAGATG
  GGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGAAACTCTATAGCCTCCTGTGC
  AGATGAACAACCTCACATCGGAAACTACAGACTGTTGAAAACAATCGGCAAGGGGAATTTTGCAAAAGTA
  AAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAA
  GTTATTCGAAGTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCTAAATTTAGACAGATTGTGT
  CTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGAGACCTCAAGGCTGAAAATCTATTGTTAGA
  TGCCGATATGAACATTAAAATAGCAGATTTCGGTTTTAGCAATGAATTTACTGTTGGCGGTAAACTCGAC
  ACGTTTTGTGGCAGTCCTCCATACGCAGCACCTGAGCTCTTCCAGGGCAAGAAATATGACGGGCCAGAAG
  TGGATGTGTGGAGTCTGGGGGTCATTTTATACACACTAGTCAGTGGCTCACTTCCCTTTGATGGGCAAAA
  CCTAAAGGAACTGAGAGAGAGAGTATTAAGAGGGAAATACAGAATTCCCTTCTACATGTCTACAGACTGT
  GAAAACCTTCTCAAACGTTTCCTGGTGCTAAATCCAATTAAACGCGGCACTCTAGAGCAAATCATGAAGG
  ACAGGTGGATCAATGCAGGGCATGAAGAAGATGAACTCAAACCATTTGTTGAACCAGAGCTAGACATCTC
  AGACCAAAAAAGAATAGATATTATGGTGGGAATGGGATATTCACAAGAAGAAATTCAAGAATCTCTTAGT
  AAGATGAAATACGATGAAATCACAGCTACATATTTGTTATTGGGGAGAAAATCTTCAGAGCTGGATGCTA
  GTGATTCCAGTTCTAGCAGCAATCTTTCACTTGCTAAGGTTAGGCCGAGCAGTGATCTCAACAACAGTAC
  TGGCCAGTCTCCTCACCACAAAGTGCAGAGAAGTGTTTCTTCAAGCCAAAAGCAAAGACGCTACAGTGAC
  CATGCTGGACCAGCTATTCCTTCTGTTGTGGCGTATCCGAAAAGGAGTCAGACCAGCACTGCAGATAGTG
  ACCTCAAAGAAGATGGAATTTCCTCCCGGAAATCAAGTGGCAGTGCTGTTGGAGGAAAGGGAATTGCTCC
  AGCCAGTCCCATGCTTGGGAATGCAAGTAATCCTAATAAGGCGGATATTCCTGAACGCAAGAAAAGCTCC
  ACTGTCCCTAGTAGTAACACAGCATCTGGTGGAATGACACGACGAAATACTTATGTTTGCAGTGAGAGAA
  CTACAGCTGATAGACACTCAGTGATTCAGAATGGCAAAGAAAACAGCACTATTCCTGATCAGAGAACTCC
  AGTTGCTTCAACACACAGTATCAGTAGTGCAGCCACCCCAGATCGAATCCGCTTCCCAAGAGGCACTGCC
  AGTCGTAGCACTTTCCACGGCCAGCCCCGGGAACGGCGAACCGCAACATATAATGGCCCTCCTGCCTCTC
  CCAGCCTGTCCCATGAAGCCACACCATTGTCCCAGACTCGAAGCCGAGGCTCCACTAATCTCTTTAGTAA
  ATTAACTTCAAAACTCACAAGGAGTCGCAATGTATCTGCTGAGCAAAAAGATGAAAACAAAGAAGCAAAG
  CCTCGATCCCTACGCTTCACCTGGAGCATGAAAACCACTAGTTCAATGGATCCCGGGGACATGATGCGGG
  AAATCCGCAAAGTGTTGGACGCCAATAACTGCGACTATGAGCAGAGGGAGCGCTTCTTGCTCTTCTGCGT
  CCACGGAGATGGGCACGCGGAGAACCTCGTGCAGTGGGAAATGGAAGTGTGCAAGCTGCCAAGACTGTCT
  CTGAACGGGGTCCGGTTTAAGCGGATATCGGGGACATCCATAGCCTTCAAAAATATTGCTTCCAAAATTG
  CCAATGAGCTAAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205758 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTRTPLPTVNERDTENHTSHGDGRQEVTSRTSRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKV
  KLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEV
  FDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGGKLD
  TFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDC
  ENLLKRFLVLNPIKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLS
  KMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRPSSDLNNSTGQSPHHKVQRSVSSSQKQRRYSD
  HAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPNKADIPERKKSS
  TVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTA
  SRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRSRNVSAEQKDENKEAK
  PRSLRFTWSMKTTSSMDPGDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLS
  LNGVRFKRISGTSIAFKNIASKIANELKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002376
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002376.6
• RefSeq Size: 3474 bp
• RefSeq ORF: 2190 bp
• Locus ID: 4140
• UniProt ID: P27448
• Cytogenetics: 14q32.32-q32.33
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 81.4 kDa
• Gene Summary: The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]