SPRING1 (NM_024738) Human Tagged ORF Clone
CAT#: RC205743
C12orf49 (Myc-DDK-tagged)-Human chromosome 12 open reading frame 49 (C12orf49)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_024738" in other vectors (6)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SPRING1 |
Synonyms | C12orf49; LUR1; POST1; SPRING |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205743 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACCTGGCGGCCATGGTGTGGCGCCGGCTTCTGCGGAAGAGGTGGGTGCTCGCCCTGGTCTTCG GGCTGTCGCTCGTCTACTTCCTCAGCAGCACCTTCAAGCAGGAGGAGAGGGCAGTGAGAGATAGGAATCT CCTCCAGGTTCATGACCATAATCAGCCCATCCCGTGGAAAGTGCAGTTTAACTTGGGCAATAGCAGTCGT CCGAGCAATCAGTGCCGCAACTCCATTCAAGGGAAGCACCTCATCACGGATGAACTCGGCTACGTTTGCG AGAGGAAGGATTTGCTGGTAAATGGCTGCTGTAATGTCAACGTCCCTAGCACGAAGCAGTACTGCTGTGA TGGCTGCTGGCCCAACGGCTGCTGCAGCGCCTATGAGTACTGTGTCTCCTGCTGCCTGCAGCCCAACAAG CAACTTCTCCTGGAGCGCTTCCTCAACCGGGCAGCCGTGGCATTCCAGAACCTCTTCATGGCAGTCGAAG ATCACTTTGAGTTGTGCCTGGCCAAATGCAGGACCTCATCTCAGAGCGTGCAGCATGAGAACACCTACCG GGACCCCATAGCAAAGTATTGCTATGGAGAAAGCCCGCCCGAGCTCTTCCCCGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205743 protein sequence
Red=Cloning site Green=Tags(s) MVNLAAMVWRRLLRKRWVLALVFGLSLVYFLSSTFKQEERAVRDRNLLQVHDHNQPIPWKVQFNLGNSSR PSNQCRNSIQGKHLITDELGYVCERKDLLVNGCCNVNVPSTKQYCCDGCWPNGCCSAYEYCVSCCLQPNK QLLLERFLNRAAVAFQNLFMAVEDHFELCLAKCRTSSQSVQHENTYRDPIAKYCYGESPPELFPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_024738 |
ORF Size | 615 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024738.4 |
RefSeq Size | 2798 bp |
RefSeq ORF | 618 bp |
Locus ID | 79794 |
UniProt ID | Q9H741 |
Cytogenetics | 12q24.22 |
Protein Families | Transmembrane |
MW | 23.6 kDa |
Gene Summary | Positively regulates hepatic SREBP signaling pathway by modulating the proper localization of SCAP (SREBP cleavage-activating protein) to the endoplasmic reticulum, thereby controlling the level of functional SCAP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205743L1 | Lenti ORF clone of Human chromosome 12 open reading frame 49 (C12orf49), Myc-DDK-tagged |
USD 600.00 |
|
RC205743L2 | Lenti ORF clone of Human chromosome 12 open reading frame 49 (C12orf49), mGFP tagged |
USD 600.00 |
|
RC205743L3 | Lenti ORF clone of Human chromosome 12 open reading frame 49 (C12orf49), Myc-DDK-tagged |
USD 600.00 |
|
RC205743L4 | Lenti ORF clone of Human chromosome 12 open reading frame 49 (C12orf49), mGFP tagged |
USD 600.00 |
|
RG205743 | C12orf49 (tGFP-tagged) - Human chromosome 12 open reading frame 49 (C12orf49) |
USD 500.00 |
|
SC112029 | C12orf49 (untagged)-Human chromosome 12 open reading frame 49 (C12orf49) |
USD 300.00 |
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