Adipose Triglyceride Lipase (PNPLA2) (NM_020376) Human Tagged ORF Clone

CAT#: RC205708

PNPLA2 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 2 (PNPLA2)

Product Images

Circular map for RC205708

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  Western blot validation of overexpression lysate (Cat# LY402777) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205708 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PNPLA2 protein (Cat# TP305708). The protein was produced from HEK293T cells transfected with PNPLA2 cDNA clone (Cat# RC205708) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adipose Triglyceride Lipase
• Synonyms: 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; TTS-2.2; TTS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205708 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCCCGCGAGAAGACGTGGAACATCTCGTTCGCGGGCTGCGGCTTCCTCGGCGTCTACTACGTCG
  GCGTGGCCTCCTGCCTCCGCGAGCACGCGCCCTTCCTGGTGGCCAACGCCACGCACATCTACGGCGCCTC
  GGCCGGGGCGCTCACGGCCACGGCGCTGGTCACCGGGGTCTGCCTGGGTGAGGCTGGTGCCAAGTTCATT
  GAGGTATCTAAAGAGGCCCGGAAGCGGTTCCTGGGCCCCCTGCACCCCTCCTTCAACCTGGTAAAGATCA
  TCCGCAGTTTCCTGCTGAAGGTCCTGCCTGCTGATAGCCATGAGCATGCCAGTGGGCGCCTGGGCATCTC
  CCTGACCCGCGTGTCAGACGGCGAGAATGTCATTATATCCCACTTCAACTCCAAGGACGAGCTCATCCAG
  GCCAATGTCTGCAGCGGTTTCATCCCCGTGTACTGTGGGCTCATCCCTCCCTCCCTCCAGGGGGTGCGCT
  ACGTGGATGGTGGCATTTCAGACAACCTGCCACTCTATGAGCTTAAGAACACCATCACAGTGTCCCCCTT
  CTCGGGCGAGAGTGACATCTGTCCGCAGGACAGCTCCACCAACATCCACGAGCTGCGGGTCACCAACACC
  AGCATCCAGTTCAACCTGCGCAACCTCTACCGCCTCTCCAAGGCCCTCTTCCCGCCGGAGCCCCTGGTGC
  TGCGAGAGATGTGCAAGCAGGGATACCGGGATGGCCTGCGCTTTCTGCAGCGGAACGGCCTCCTGAACCG
  GCCCAACCCCTTGCTGGCGTTGCCCCCCGCCCGCCCCCACGGCCCAGAGGACAAGGACCAGGCAGTGGAG
  AGCGCCCAAGCGGAGGATTACTCGCAGCTGCCGGGAGAAGATCACATCCTGGAGCACCTGCCCGCCCGGC
  TCAATGAGGCCCTGCTGGAGGCCTGCGTGGAGCCCACGGACCTGCTGACCACCCTCTCCAACATGCTGCC
  TGTGCGTCTGGCCACGGCCATGATGGTGCCCTACACGCTGCCGCTGGAGAGCGCTCTGTCCTTCACCATC
  CGCTTGCTGGAGTGGCTGCCCGACGTTCCCGAGGACATCCGGTGGATGAAGGAGCAGACGGGCAGCATCT
  GCCAGTACCTGGTGATGCGCGCCAAGAGGAAGCTGGGCAGGCACCTGCCCTCCAGGCTGCCGGAGCAGGT
  GGAGCTGCGCCGCGTCCAGTCGCTGCCGTCCGTGCCGCTGTCCTGCGCCGCCTACAGAGAGGCACTGCCC
  GGCTGGATGCGCAACAACCTCTCGCTGGGGGACGCGCTGGCCAAGTGGGAGGAGTGCCAGCGCCAGCTGC
  TGCTCGGCCTCTTCTGCACCAACGTGGCCTTCCCGCCCGAAGCTCTGCGCATGCGCGCACCCGCCGACCC
  GGCTCCCGCCCCCGCGGACCCAGCATCCCCGCAGCACCAGCTGGCCGGGCCTGCCCCCTTGCTGAGCACC
  CCTGCTCCCGAGGCCCGGCCCGTGATCGGGGCCCTGGGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205708 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPREKTWNISFAGCGFLGVYYVGVASCLREHAPFLVANATHIYGASAGALTATALVTGVCLGEAGAKFI
  EVSKEARKRFLGPLHPSFNLVKIIRSFLLKVLPADSHEHASGRLGISLTRVSDGENVIISHFNSKDELIQ
  ANVCSGFIPVYCGLIPPSLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT
  SIQFNLRNLYRLSKALFPPEPLVLREMCKQGYRDGLRFLQRNGLLNRPNPLLALPPARPHGPEDKDQAVE
  SAQAEDYSQLPGEDHILEHLPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTI
  RLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLPEQVELRRVQSLPSVPLSCAAYREALP
  GWMRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPEALRMRAPADPAPAPADPASPQHQLAGPAPLLST
  PAPEARPVIGALGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020376
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020376.2, NP_065109.1
• RefSeq Size: 2443 bp
• RefSeq ORF: 1515 bp
• Locus ID: 57104
• UniProt ID: Q96AD5
• Cytogenetics: 11p15.5
• Protein Families: Transmembrane
• MW: 55.3 kDa
• Gene Summary: This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]