EDEM1 (NM_014674) Human Tagged ORF Clone

CAT#: RC205655

EDEM1 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1)

Product Images

Circular map for RC205655

• 

  Western blot validation of overexpression lysate (Cat# LY415128) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205655 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EDEM1
• Synonyms: EDEM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205655 representing NM_014674
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTATTGTGGCTCG
  TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGCTTCGGCTTCCAGCGTCTGAG
  GAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTGGGCCGGCCTGGGGGGGTATCCGGGCCGTCG
  TGGCTGCAGCCGCCGGGGACCGGGGCAGCGCAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGA
  TGTGCGGCCCAGCCAACTGGGGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCG
  CTACAGCGGCGCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTT
  GGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGCCGCGGCCGTG
  GGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGGAACTACTCATTGACTCTTGT
  TGATGCATTGGATACACTTGCAATAATGGGAAATTCATCCGAGTTCCAGAAAGCAGTCAAGTTAGTGATC
  AACACAGTTTCATTTGACAAAGATTCCACCGTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCC
  TCCTTTCTGCTCACAGAATAATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGA
  TAATGAGTTGTTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACA
  GGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAGACATGCACAG
  CGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTGGGGGACTCCACATTTGAGTG
  GGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGGAGCAATGATACAGGATTACTAGGCAATGTC
  GTGAACATTCAGACGGGCCACTGGGTTGGAAAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATG
  AATACCTCTTGAAATCTTACATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCA
  GAGTATTCAGAACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTAT
  GTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCCTTTTTCCCTG
  GACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCCTTCTACTATGCCATATGGAA
  ACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTGCAGGCCCCTGACGTTCTCTTCTACCCACTG
  AGACCAGAGTTAGTGGAATCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAG
  GAATGGATATTCTGCAGAGTCTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGT
  CATTGACAAGTCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTG
  CTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAGGGACACATTG
  TATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCTGAAGAGGGAGGGCAGGACCA
  AGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTAAAAGTCATCAACTCCAGCTCCAACTGCAAT
  CGTGTACCTGATGAGAGGAGGTACTCCCTGCCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGG
  TTGGTTTGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205655 representing NM_014674
  Red=Cloning site Green=Tags(s)
  
  MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPVGRPGGVSGPS
  WLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVF
  GYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVI
  NTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKT
  GIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNV
  VNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLY
  VNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPL
  RPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYL
  LFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCN
  RVPDERRYSLPLKSIYMRQIDQMVGLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014674
• ORF Size: 1971 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014674.1, NP_055489.1
• RefSeq Size: 6072 bp
• RefSeq ORF: 1974 bp
• Locus ID: 9695
• UniProt ID: Q92611
• Cytogenetics: 3p26.1
• Domains: Glyco_hydro_47
• Protein Families: Transmembrane
• MW: 73.6 kDa
• Gene Summary: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.[UniProtKB/Swiss-Prot Function]