EDEM1 (NM_014674) Human Tagged ORF Clone

CAT#: RC205655

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EDEM1 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1)



  "NM_014674" in other vectors (4)

Reconstitution Protocol

USD 917.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol EDEM1
Synonyms EDEM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205655 representing NM_014674
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTATTGTGGCTCG
TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGCTTCGGCTTCCAGCGTCTGAG
GAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTGGGCCGGCCTGGGGGGGTATCCGGGCCGTCG
TGGCTGCAGCCGCCGGGGACCGGGGCAGCGCAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGA
TGTGCGGCCCAGCCAACTGGGGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCG
CTACAGCGGCGCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTT
GGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGCCGCGGCCGTG
GGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGGAACTACTCATTGACTCTTGT
TGATGCATTGGATACACTTGCAATAATGGGAAATTCATCCGAGTTCCAGAAAGCAGTCAAGTTAGTGATC
AACACAGTTTCATTTGACAAAGATTCCACCGTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCC
TCCTTTCTGCTCACAGAATAATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGA
TAATGAGTTGTTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACA
GGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAGACATGCACAG
CGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTGGGGGACTCCACATTTGAGTG
GGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGGAGCAATGATACAGGATTACTAGGCAATGTC
GTGAACATTCAGACGGGCCACTGGGTTGGAAAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATG
AATACCTCTTGAAATCTTACATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCA
GAGTATTCAGAACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTAT
GTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCCTTTTTCCCTG
GACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCCTTCTACTATGCCATATGGAA
ACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTGCAGGCCCCTGACGTTCTCTTCTACCCACTG
AGACCAGAGTTAGTGGAATCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAG
GAATGGATATTCTGCAGAGTCTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGT
CATTGACAAGTCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTG
CTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAGGGACACATTG
TATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCTGAAGAGGGAGGGCAGGACCA
AGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTAAAAGTCATCAACTCCAGCTCCAACTGCAAT
CGTGTACCTGATGAGAGGAGGTACTCCCTGCCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGG
TTGGTTTGATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205655 representing NM_014674
Red=Cloning site Green=Tags(s)

MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPVGRPGGVSGPS
WLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVF
GYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVI
NTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKT
GIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNV
VNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLY
VNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPL
RPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYL
LFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCN
RVPDERRYSLPLKSIYMRQIDQMVGLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014674
ORF Size 1971 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014674.1, NP_055489.1
RefSeq Size 6072 bp
RefSeq ORF 1974 bp
Locus ID 9695
UniProt ID Q92611
Cytogenetics 3p26.1
Domains Glyco_hydro_47
Protein Families Transmembrane
MW 73.6 kDa
Gene Summary Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.[UniProtKB/Swiss-Prot Function]

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