FGD2 (NM_173558) Human Tagged ORF Clone

CAT#: RC205644

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FGD2 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 2 (FGD2)



  "NM_173558" in other vectors (4)

Reconstitution Protocol

USD 610.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FGD2
Synonyms ZFYVE4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205644 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGGGCAAGTGAGGAGAAGCTGGCATCTGTGTCCAACCTGGTCACTGTGTTTGAGAATAGCAGGA
CCCCAGAAGCAGCACCCAGAGGCCACAGGCTAGAGGACGTGCATCACCGCCCTGAGTGCAGGCCTCCCGA
GTCCCCAGGACCACGGGAGAAGACGAATGTCGGGGAGGCCGTGGGGTCTGAGCCCAGGACAGTCAGCAGG
AGGTACCTGAACTCCCTGAAGAACAAGCTGTCCAGCGAAGCCTGGAGGAAATCTTGCCAGCCTGTGACCC
TCTCAGGATCGGGGACGCAGGAGCCAGAGAAGAAGATCGTCCAGGAGCTGCTGGAGACAGAGCAGGCCTA
TGTGGCGCGCCTCCACCTGCTAGACCAGGTGTTTTTCCAGGAGCTGCTGAAGACAGCCCGCAGCAGCAAG
GCCTTCCCAGAGGATGTGGTCAGGGTCATCTTCTCCAACATCTCCTCCATCTATCAGTTCCATTCTCAGT
TCTTCCTCCCAGAGCTGCAGCGGCGCCTGGACGACTGGACAGCTAACCCCCGCATCGGTGACGTGATCCA
GAAGCTGGCCCCCTTCCTGAAGATGTACAGTGAGTATGTCAAGAACTTTGAGCGAGCGGCTGAGCTGCTG
GCCACCTGGACCGACAAGTCTCCACTCTTCCAGGAGGTTCTCACTCGCATCCAGAGCAGCGAGGCTTCGG
GCAGCCTGACCCTGCAGCACCACATGCTGGAACCAGTGCAGAGAATTCCACGTTACGAGCTGCTGCTCAA
GGAGTACATCCAGAAGCTGCCAGCCCAGGCCCCAGACCAGGCCGATGCCCAGAAAGCCCTGGACATGATC
TTCTCAGCTGCCCAGCACTCCAATGCAGCCATCACTGAGATGGAGCGGCTGCAGGACCTGTGGGAGGTGT
ACCAGCGCCTGGGCCTCGAGGACGACATAGTAGACCCCTCTAACACCCTGCTCCGTGAGGGCCCGGTCCT
CAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTACCTTTTCTTGTTCAACAACATGCTGCTCTAC
TGTGTGCCCAGGGTGATCCAGGTGGGCGCCCAGTTCCAGGTGAGGACCCGCATCGATGTGGCCGGGATGA
AGGTGCGGGAGCTGATGGATGCTGAGTTTCCCCACTCCTTCCTGGTGTCCGGGAAGCAGCGCACCCTGGA
GCTGCAAGCCCGGTCCCAGGAGGAAATGATTTCCTGGATGCAGGCCTTCCAAGCAGCCATTGACCAAATC
GAGAAGCGGAATGAAACCTTCAAGGCTGCGGCCCAGGGGCCTGAGGGAGACATCCAGGAGCAGGAGCTGC
AGTCTGAGGAGCTGGGCCTCCGGGCACCGCAGTGGGTCCGGGACAAGATGGTGACCATGTGCATGCGCTG
CCAGGAGCCCTTCAACGCTCTGACGCGCCGTCGCCACCACTGCCGGGCCTGCGGCTATGTGGTGTGTGCC
AGGTGCTCCGACTACCGGGCCGAACTGAAATACGACGACAACAGGCCCAACCGAGTCTGCCTCCACTGCT
ACGCATTCCTCACTGGAAATGTGCTGCCTGAGGCCAAGGAGGACAAGAGGCGGGGCATCCTGGAGAAAGG
GTCCTCAGCCACGCCTGACCAGAGCCTGATGTGCAGCTTCCTGCAGCTCATCGGGGACAAGTGGGGCAAG
AGCGGCCCCCGGGGCTGGTGTGTGATCCCTCGGGATGACCCCCTCGTGCTCTATGTCTATGCTGCCCCTC
AGGACATGAGGGCTCACACCTCCATCCCCCTGCTGGGCTACCAGGTGACTGTTGGGCCCCAGGGGGACCC
TCGGGTCTTCCAGCTACAGCAGTCAGGCCAGCTCTACACCTTCAAGGCCGAGACGGAGGAGCTGAAGGGC
CGCTGGGTGAAGGCCATGGAGCGGGCGGCCAGTGGCTGGAGCCCCAGCTGGCCCAACGATGGGGACCTGT
CCGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205644 protein sequence
Red=Cloning site Green=Tags(s)

MKGASEEKLASVSNLVTVFENSRTPEAAPRGHRLEDVHHRPECRPPESPGPREKTNVGEAVGSEPRTVSR
RYLNSLKNKLSSEAWRKSCQPVTLSGSGTQEPEKKIVQELLETEQAYVARLHLLDQVFFQELLKTARSSK
AFPEDVVRVIFSNISSIYQFHSQFFLPELQRRLDDWTANPRIGDVIQKLAPFLKMYSEYVKNFERAAELL
ATWTDKSPLFQEVLTRIQSSEASGSLTLQHHMLEPVQRIPRYELLLKEYIQKLPAQAPDQADAQKALDMI
FSAAQHSNAAITEMERLQDLWEVYQRLGLEDDIVDPSNTLLREGPVLKISFRRNDPMERYLFLFNNMLLY
CVPRVIQVGAQFQVRTRIDVAGMKVRELMDAEFPHSFLVSGKQRTLELQARSQEEMISWMQAFQAAIDQI
EKRNETFKAAAQGPEGDIQEQELQSEELGLRAPQWVRDKMVTMCMRCQEPFNALTRRRHHCRACGYVVCA
RCSDYRAELKYDDNRPNRVCLHCYAFLTGNVLPEAKEDKRRGILEKGSSATPDQSLMCSFLQLIGDKWGK
SGPRGWCVIPRDDPLVLYVYAAPQDMRAHTSIPLLGYQVTVGPQGDPRVFQLQQSGQLYTFKAETEELKG
RWVKAMERAASGWSPSWPNDGDLSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173558
ORF Size 1965 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173558.2, NP_775829.1
RefSeq Size 3045 bp
RefSeq ORF 1968 bp
Locus ID 221472
UniProt ID Q7Z6J4
Cytogenetics 6p21.2
MW 74.9 kDa
Gene Summary The protein encoded by this gene belongs to a family of guanine nucleotide exchange factors (GEFs) which control cytoskeleton-dependent membrane rearrangements by activating the cell division cycle 42 (CDC42) protein. This gene is expressed in B lymphocytes, macrophages, and dendritic cells. The encoded protein may play a role in leukocyte signaling and vesicle trafficking in antigen-presenting cells in the immune system. [provided by RefSeq, Oct 2016]

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