ZNF165 (NM_003447) Human Tagged ORF Clone

CAT#: RC205600

ZNF165 (Myc-DDK-tagged)-Human zinc finger protein 165 (ZNF165)

Product Images

Circular map for RC205600

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF165 (Cat# RC205600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF165(Cat# TA800423). Positive lysates LY418674 (100ug) and LC418674 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418674) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205600 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZNF165 protein (Cat# TP305600). The protein was produced from HEK293T cells transfected with ZNF165 cDNA clone (Cat# RC205600) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF165
• Synonyms: CT53; LD65; ZSCAN7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205600 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAACCAAAGAAAGCTGCAGCCCAGAACTCTCCAGAGGATGAAGGACTTCTGATAGTGAAGA
  TAGAAGAGGAAGAATTTATCCATGGGCAGGACACTTGCTTACAGAGAAGTGAACTCCTTAAGCAGGAGCT
  CTGCAGGCAGCTTTTTAGGCAGTTCTGCTACCAGGATTCTCCTGGACCTCGCGAGGCACTGAGCCGCCTC
  CGGGAGCTCTGCTGTCAGTGGCTGAAGCCAGAGATCCATACCAAGGAACAGATTCTGGAACTGCTGGTGC
  TAGAGCAGTTCCTGACCATCCTGCCAGGAGATTTGCAGGCCTGGGTACATGAACATTACCCAGAGAGTGG
  AGAGGAGGCAGTGACCATACTAGAAGATTTGGAGAGAGGCACTGATGAAGCAGTACTCCAGGTTCAAGCC
  CATGAACATGGACAAGAAATATTCCAGAAAAAAGTGTCACCTCCTGGACCAGCACTTAATGTCAAGTTAC
  AGCCAGTGGAGACCAAGGCCCATTTTGATTCATCAGAACCCCAGCTCCTATGGGACTGTGATAATGAGAG
  TGAAAACAGTAGATCCATGCCAAAGCTGGAAATTTTTGAAAAAATTGAATCACAGAGAATTATATCTGGA
  AGAATCTCAGGATACATATCAGAAGCGTCTGGTGAGTCTCAAGACATCTGTAAGTCTGCAGGCAGGGTAA
  AGAGACAATGGGAAAAAGAATCAGGGGAGTCTCAGAGACTCTCGTCTGCCCAGGATGAAGGTTTTGGTAA
  AATCCTCACCCACAAAAATACAGTCAGAGGTGAAATAATAAGCCACGATGGATGTGAGAGGAGATTAAAT
  CTGAACTCAAATGAATTCACACACCAGAAATCTTGTAAACATGGTACCTGTGACCAGAGCTTCAAATGGA
  ACTCAGATTTTATTAACCATCAAATAATTTATGCTGGAGAAAAAAATCACCAATATGGAAAATCTTTCAA
  GAGCCCAAAACTTGCTAAACATGCAGCAGTTTTCAGTGGAGATAAAACTCATCAGTGTAATGAATGTGGG
  AAAGCTTTCAGGCACAGCTCAAAACTTGCTAGGCATCAGAGAATCCACACTGGAGAGAGATGCTATGAAT
  GTAATGAATGTGGGAAAAGCTTTGCAGAGAGCTCAGATCTTACTAGACATCGGCGAATTCACACTGGGGA
  AAGACCCTTTGGTTGCAAAGAATGTGGGAGAGCATTCAACCTGAACTCACATCTTATCAGGCATCAGAGA
  ATTCACACCAGAGAGAAACCCTACGAGTGTAGTGAATGTGGGAAAACCTTCCGAGTGAGCTCACATCTTA
  TTCGACACTTTAGAATTCACACTGGAGAAAAACCCTATGAATGCAGTGAGTGTGGAAGAGCCTTCAGTCA
  GAGCTCAAACCTTAGTCAACACCAGAGAATTCACATGAGGGAAAACCTATTAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205600 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATEPKKAAAQNSPEDEGLLIVKIEEEEFIHGQDTCLQRSELLKQELCRQLFRQFCYQDSPGPREALSRL
  RELCCQWLKPEIHTKEQILELLVLEQFLTILPGDLQAWVHEHYPESGEEAVTILEDLERGTDEAVLQVQA
  HEHGQEIFQKKVSPPGPALNVKLQPVETKAHFDSSEPQLLWDCDNESENSRSMPKLEIFEKIESQRIISG
  RISGYISEASGESQDICKSAGRVKRQWEKESGESQRLSSAQDEGFGKILTHKNTVRGEIISHDGCERRLN
  LNSNEFTHQKSCKHGTCDQSFKWNSDFINHQIIYAGEKNHQYGKSFKSPKLAKHAAVFSGDKTHQCNECG
  KAFRHSSKLARHQRIHTGERCYECNECGKSFAESSDLTRHRRIHTGERPFGCKECGRAFNLNSHLIRHQR
  IHTREKPYECSECGKTFRVSSHLIRHFRIHTGEKPYECSECGRAFSQSSNLSQHQRIHMRENLLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003447
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003447.4
• RefSeq Size: 2411 bp
• RefSeq ORF: 1458 bp
• Locus ID: 7718
• UniProt ID: P49910
• Cytogenetics: 6p22.1
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• MW: 55.8 kDa
• Gene Summary: This gene encodes a member of the Kruppel family of zinc finger proteins. Members of this DNA-binding protein family act as transcriptional regulators. This gene is located within a cluster of zinc finger family members. The encoded protein may play a role in spermatogenesis. [provided by RefSeq, Jul 2008]