TKTL2 (NM_032136) Human Tagged ORF Clone

CAT#: RC205557

TKTL2 (Myc-DDK-tagged)-Human transketolase-like 2 (TKTL2)

Product Images

Circular map for RC205557

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  Western blot validation of overexpression lysate (Cat# LY410329) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205557 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TKTL2 protein (Cat# TP305557). The protein was produced from HEK293T cells transfected with TKTL2 cDNA clone (Cat# RC205557) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TKTL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205557 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCAACGACGCCAAGCCCGACGTGAAGACCGTGCAGGTGCTGCGGGACACAGCCAACCGCCTGC
  GGATCCATTCCATCAGGGCCACGTGTGCCTCTGGTTCTGGCCAGCTCACGTCGTGCTGCAGTGCAGCGGA
  GGTCGTGTCTGTCCTCTTCTTCCACACGATGAAGTATAAACAGACAGACCCAGAACACCCGGACAACGAC
  CGGTTCATCCTCTCCAGGGGACATGCTGCTCCTATCCTCTATGCTGCTTGGGTGGAGGTGGGTGACATCA
  GTGAATCTGACTTGCTGAACCTGAGGAAACTTCACAGCGACTTGGAGAGACACCCTACCCCCCGATTGCC
  GTTTGTTGACGTGGCAACAGGGTCCCTAGGTCAGGGATTAGGTACTGCATGTGGAATGGCTTATACTGGC
  AAGTACCTTGACAAGGCCAGCTACCGGGTGTTCTGCCTTATGGGAGATGGCGAATCCTCAGAAGGCTCTG
  TGTGGGAGGCTTTTGCTTTTGCCTCCCACTACAACTTGGACAATCTCGTGGCGGTCTTCGACGTGAACCG
  CTTGGGACAAAGTGGCCCTGCACCCCTTGAGCATGGCGCAGACATCTACCAGAATTGCTGTGAAGCCTTT
  GGATGGAATACTTACTTAGTGGATGGCCATGATGTGGAGGCCTTGTGCCAAGCATTTTGGCAAGCAAGTC
  AAGTGAAGAACAAGCCTACTGCTATAGTTGCCAAGACCTTCAAAGGTCGGGGTATTCCAAATATTGAGGA
  TGCAGAAAATTGGCATGGAAAGCCAGTGCCAAAAGAAAGAGCAGATGCAATTGTCAAATTAATTGAGAGT
  CAGATACAGACCAATGAGAATCTCATACCAAAATCGCCTGTGGAAGACTCACCTCAAATAAGCGTCACAG
  ATATAAAAATGACCTCCCCACATGCTTACAAAGTTGGTGACAAGATAGCTACTCAGAAAACATATGGTTT
  GGCTCTGGCTAAACTGGGCCGTGCAAATGAAAGAGTTATTGTTCTGAGTGGTGACACGATGAACTCCACC
  TTTTCTGAGATATTCAGGAAAGAACACCCTGAGCGTTTCATAGAGTGTATTATTGCTGAACAAAACATGG
  TAAGTGTGGCACTAGGCTGTGCTACACGTGGTCGAACCATTGCTTTTGCTGGTGCTTTTGCTGCCTTTTT
  TACTAGAGCATTCGATCAGCTCCGAATGGGAGCCATTTCTCAAGCCAATATCAACCTTATTGGTTCCCAC
  TGTGGGGTATCCACTGGAGAAGATGGAGTCTCCCAGATGGCCCTGGAGGATCTAGCCATGTTCCGAAGCA
  TTCCCAATTGTACTGTTTTCTATCCAAGTGATGCCATCTCGACAGAGCATGCTATTTATCTAGCCGCCAA
  TACCAAGGGAATGTGCTTCATTCGAACCAGCCAACCAGAAACTGCAGTTATTTATACCCCACAAGAAAAT
  TTTGAGATTGGCCAGGCCAAGGTGGTCCGCCACGGTGTCAATGATAAAGTCACAGTAATTGGAGCTGGAG
  TTACTCTCCATGAAGCCTTAGAAGCTGCTGACCATCTTTCTCAACAAGGTATTTCTGTCCGTGTCATCGA
  CCCATTTACCATTAAACCCCTGGATGCCGCCACCATCATCTCCAGTGCAAAAGCCACAGGCGGCCGAGTT
  ATCACAGTGGAGGATCACTACAGGGAAGGTGGCATTGGAGAAGCTGTTTGTGCAGCTGTCTCCAGGGAGC
  CTGATATCCTTGTTCATCAACTGGCAGTGTCAGGAGTGCCTCAACGTGGGAAAACTAGTGAATTGCTGGA
  TATGTTTGGAATCAGTACCAGACACATTATAGCAGCCGTAACACTTACTTTAATGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205557 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMANDAKPDVKTVQVLRDTANRLRIHSIRATCASGSGQLTSCCSAAEVVSVLFFHTMKYKQTDPEHPDND
  RFILSRGHAAPILYAAWVEVGDISESDLLNLRKLHSDLERHPTPRLPFVDVATGSLGQGLGTACGMAYTG
  KYLDKASYRVFCLMGDGESSEGSVWEAFAFASHYNLDNLVAVFDVNRLGQSGPAPLEHGADIYQNCCEAF
  GWNTYLVDGHDVEALCQAFWQASQVKNKPTAIVAKTFKGRGIPNIEDAENWHGKPVPKERADAIVKLIES
  QIQTNENLIPKSPVEDSPQISVTDIKMTSPHAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNST
  FSEIFRKEHPERFIECIIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSH
  CGVSTGEDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPETAVIYTPQEN
  FEIGQAKVVRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFTIKPLDAATIISSAKATGGRV
  ITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRGKTSELLDMFGISTRHIIAAVTLTLMK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032136
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032136.3, NP_115512.2
• RefSeq Size: 2837 bp
• RefSeq ORF: 1881 bp
• Locus ID: 84076
• UniProt ID: Q9H0I9
• Cytogenetics: 4q32.2
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway
• MW: 67.9 kDa
• Gene Summary: Plays an essential role in total transketolase activity and cell proliferation in cancer cells; after transfection with anti-TKTL1 siRNA, total transketolase activity dramatically decreases and proliferation was significantly inhibited in cancer cells. Plays a pivotal role in carcinogenesis.[UniProtKB/Swiss-Prot Function]