SLC22A15 (NM_018420) Human Tagged ORF Clone

CAT#: RC205541

SLC22A15 (Myc-DDK-tagged)-Human solute carrier family 22, member 15 (SLC22A15)

Product Images

Circular map for RC205541

• 

  Western blot validation of overexpression lysate (Cat# LY402683) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205541 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC22A15
• Synonyms: FLIPT1; PRO34686
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205541 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCTACCAGATGTACTTGTGCTTCCTGCTGGCCGTGCTGCTGCAGCTCTACGTGGCCACGGAGG
  CCATCCTCATTGCACTGGTTGGGGCCACGCCATCCTACCACTGGGACCTGGCAGAGCTCCTGCCAAATCA
  GAGCCACGGTAACCAGTCAGCTGGTGAAGACCAGGCCTTTGGGGACTGGCTCCTGACAGCCAACGGCAGT
  GAGATCCATAAGCACGTGCATTTCAGCAGCAGCTTCACCTCCATCGCCTCGGAGTGGTTTTTAATTGCCA
  ACAGATCCTACAAAGTCAGTGCAGCAAGCTCTTTTTTCTTCAGTGGTGTATTTGTTGGAGTTATCTCTTT
  TGGTCAGCTTTCAGATCGCTTCGGAAGGAAAAAAGTCTATCTCACAGGTTTTGCTCTTGACATCTTATTT
  GCAATTGCAAATGGATTTTCCCCCTCATATGAGTTCTTTGCAGTAACTCGCTTCCTGGTGGGCATGATGA
  ATGGAGGGATGTCGCTGGTGGCCTTTGTCTTGCTTAATGAGTGTGTGGGCACCGCCTACTGGGCACTTGC
  AGGATCGATTGGCGGCCTCTTCTTTGCAGTTGGCATTGCCCAATATGCCCTGTTAGGATACTTCATCCGC
  TCCTGGAGGACCCTAGCCATTCTGGTTAACCTGCAGGGAACGGTGGTCTTTCTCTTATCTTTATTCATTC
  CTGAATCACCTCGTTGGTTATACTCCCAGGGTCGACTGAGTGAGGCTGAAGAGGCGCTGTACCTCATTGC
  CAAGAGGAACCGCAAACTCAAGTGCACGTTCTCACTAACACACCCAGCCAACAGGAGCTGCAGGGAGACT
  GGAAGTTTCCTGGATCTCTTTCGTTACCGGGTCCTGTTAGGACACACTTTGATCCTGATGTTCATCTGGT
  TTGTGTGCAGCTTGGTGTATTATGGCCTAACTCTGAGTGCGGGTGATCTAGGTGGAAGTATTTATGCCAA
  CCTGGCCCTGTCTGGCCTCATAGAGATTCAATCTTACCCTCTCTGTATCTACTTGATTAACCAAAAATGG
  TTTGGTCGGAAGCGAACATTATCAGCATTTCTGTGCCTAGGAGGACTGGCTTGTCTTATTGTAATGTTTC
  TTCCAGAAAAGAAAGACACAGGTGTGTTTGCAGTGGTGAACAGCCATTCCTTGTCCTTGCTGGGGAAGCT
  GACCATCAGTGCTGCCTTTAACATTGTTTATATCTACACCTCTGAGCTTTACCCTACAGTCATCAGGAAT
  GTTGGGCTTGGAACTTGTTCCATGTTCTCCCGAGTTGGTGGGATTATTGCTCCCTTCATCCCCTCACTGA
  AATATGTGCAATGGTCTTTACCATTCATTGTCTTCGGAGCCACGGGTCTGACCTCCGGCCTCCTGAGTTT
  GTTATTGCCGGAGACCCTTAACAGTCCGCTGCTAGAAACATTCTCCGACCTTCAGGTGTATTCGTATCGC
  AGGCTGGGAGAAGAAGCATTATCTTTACAGGCTTTGGACCCCCAACAGTGTGTGGACAAGGAGAGCTCTT
  TAGGGAGTGAGAGTGAGGAAGAGGAAGAATTTTATGATGCAGATGAAGAGACTCAGATGATCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205541 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGIYQMYLCFLLAVLLQLYVATEAILIALVGATPSYHWDLAELLPNQSHGNQSAGEDQAFGDWLLTANGS
  EIHKHVHFSSSFTSIASEWFLIANRSYKVSAASSFFFSGVFVGVISFGQLSDRFGRKKVYLTGFALDILF
  AIANGFSPSYEFFAVTRFLVGMMNGGMSLVAFVLLNECVGTAYWALAGSIGGLFFAVGIAQYALLGYFIR
  SWRTLAILVNLQGTVVFLLSLFIPESPRWLYSQGRLSEAEEALYLIAKRNRKLKCTFSLTHPANRSCRET
  GSFLDLFRYRVLLGHTLILMFIWFVCSLVYYGLTLSAGDLGGSIYANLALSGLIEIQSYPLCIYLINQKW
  FGRKRTLSAFLCLGGLACLIVMFLPEKKDTGVFAVVNSHSLSLLGKLTISAAFNIVYIYTSELYPTVIRN
  VGLGTCSMFSRVGGIIAPFIPSLKYVQWSLPFIVFGATGLTSGLLSLLLPETLNSPLLETFSDLQVYSYR
  RLGEEALSLQALDPQQCVDKESSLGSESEEEEEFYDADEETQMIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018420
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018420.1, NP_060890.1
• RefSeq Size: 4729 bp
• RefSeq ORF: 1644 bp
• Locus ID: 55356
• UniProt ID: Q8IZD6
• Cytogenetics: 1p13.1
• Domains: sugar_tr
• Protein Families: Transmembrane
• MW: 59.3 kDa
• Gene Summary: Organic ion transporters, such as SLC22A15, transport various medically and physiologically important compounds, including pharmaceuticals, toxins, hormones, neurotransmitters, and cellular metabolites. These transporters are also referred to as amphiphilic solute facilitators (ASFs).[supplied by OMIM, Apr 2004]