Liprin alpha 1 (PPFIA1) (NM_003626) Human Tagged ORF Clone

CAT#: RC205488

PPFIA1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2

Product Images

Circular map for RC205488

• 

  Western blot validation of overexpression lysate (Cat# LY418543) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205488 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Liprin alpha 1
• Synonyms: LIP.1; LIP1; LIPRIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205488 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGCGAGGTGATGCCGACCATCAGCGAAGCAGAAGGCCCCCCTGGAGGAGGTGGAGGCCATGGTT
  CCGGCTCCCCTTCACAGCCAGATGCAGATTCACATTTTGAACAGTTGATGGTCTCCATGCTAGAAGAAAG
  GGACCGCCTTCTTGATACACTGAGAGAGACTCAAGAAACGCTGGCCTTAACCCAGGGGAAGTTACACGAG
  GTTGGTCATGAAAGAGATTCCTTGCAGAGACAGCTCAACACAGCACTTCCACAGGAGTTCGCAGCACTTA
  CTAAAGAACTCAATGTATGCAGGGAACAGCTCCTTGAAAGGGAAGAAGAAATTGCTGAACTGAAAGCAGA
  AAGGAATAACACCAGGCTGCTGTTAGAGCATTTGGAATGCCTTGTCTCCAGGCATGAGCGGTCTCTTAGG
  ATGACCGTGGTGAAGAGACAAGCGCAGTCTCCAGCAGGCGTGTCCAGCGAAGTGGAAGTGCTGAAAGCAC
  TGAAGTCCTTATTTGAACACCACAAAGCTCTGGATGAAAAGGTGAGAGAGCGATTACGAGTAGCACTTGA
  AAGATGTAGTTTGTTAGAAGAGGAATTAGGTGCCACACACAAAGAGCTAATGATTCTTAAAGAACAGAAT
  AATCAGAAAAAAACTCTAACAGATGGAGTGCTGGACATAAACCATGAACAAGAAAATACACCAAGCACGA
  GTGGAAAGAGATCTTCTGATGGTTCTTTAAGCCACGAGGAAGACCTTGCTAAAGTAATTGAGCTCCAAGA
  AATCATAAGTAAGCAGTCAAGGGAACAGAGCCAAATGAAAGAACGCCTGGCTTCCCTTTCCAGTCATGTG
  ACAGAACTGGAAGAGGATCTGGACACGGCTAGAAAAGATCTCATCAAATCTGAAGAAATGAACACAAAAT
  TGCAACGAGATGTCCGTGAAGCCATGGCCCAAAAGGAAGATATGGAAGAGAGAATCACTACTCTTGAAAA
  ACGCTACCTCGCTGCACAGCGTGAAGCCACATCTGTGCATGACCTCAATGATAAACTTGAAAATGAAATT
  GCAAATAAAGATTCTATGCATCGACAGACTGAAGATAAAAACCGCCAGTTACAGGAGCGCTTGGAATTGG
  CAGAGCAAAAGCTGCAACAGACACTGAGGAAGGCAGAGACGCTCCCGGAGGTGGAGGCGGAGCTGGCCCA
  GAGGGTGGCAGCGCTTTCCAAGGCTGAAGAGAGACACGGCAACATTGAAGAAAGGTTACGACAGATGGAA
  GCACAGTTGGAGGAGAAGAATCAAGAACTGCAGCGGGCAAGGCAAAGAGAAAAAATGAACGAAGAACATA
  ATAAACGTTTATCAGACACTGTTGACAAGCTGCTTTCAGAATCTAATGAGAGGCTTCAACTTCATCTTAA
  AGAGAGAATGGCTGCTTTGGAAGATAAGAACTCTCTTTTAAGAGAAGTTGAAAGTGCAAAAAAGCAGTTA
  GAAGGAACACAACACGATAAGGATCAGCTTGTCCTAAACATTGAAGCACTGAGGGCTGAACTAGACCACA
  TGAGACTAAGAGGTGCTTCACTTCATCATGGCCGACCCCACTTGGGCAGTGTCCCAGATTTCAGGTTCCC
  CATGGCAGATGGCCACACAGACTCCTACAGCACCAGTGCAGTGCTGCGGCGCACACAGAAAGGCCGGCTG
  GCAGCCCTGCGAGATGAGCCTTCCAAGGTACAAACTCTTAATGAGCAGGATTGGGAACGTGCCCAGCAAG
  CTAGTGTCTTGGCAAATGTAGCACAAGCATTCGAGAGTGATGCTGACGTGTCTGATGGTGAAGATGACAG
  GGACACTCTCCTCAGCTCAGTTGACCTGCTATCGCCCAGCGGGCAGGCCGACGCGCACACACTAGCCATG
  ATGCTTCAGGAGCAGCTGGACGCCATCAACAAAGAGATCAGGTTGATTCAGGAAGAAAAAGAAAATACAG
  AGCAGCGGGCAGAGGAGATTGAAAGTCGAGTTGGCAGTGGAAGTCTAGACAATCTTGGTCGTTTTAGATC
  AATGAGCTCCATTCCCCCCTACCCTGCTTCCTCGCTTGCTAGCTCCTCCCCTCCGGGCAGTGGGCGCTCC
  ACCCCACGAAGGATCCCTCACAGCCCAGCTCGGGAAGTGGACAGACTGGGCGTCATGACCCTTTTGCCAC
  CTTCCAGAGAAGAGGTACGAGATGACAAGACAACCATAAAGTGTGAAACCTCCCCGCCTTCCTCCCCGAG
  AGCCCTTCGGTTAGACCGGCTGCACAAAGGGGCGCTGCACACCGTTAGCCACGAGGACATCAGGGACATA
  AGGAACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAACCCCAGCAGTAGCAACAGTAGCCAGGACTCGC
  TCCACAAAGCCCCAAAGAAGAAAGGCATTAAGTCCTCCATTGGCCGCTTGTTTGGCAAGAAAGAAAAGGG
  CCGACCTGGACAAACTGGCAAAGAAGCATTAGGACAAGCTGGTGTTTCCGAGACGGATAACTCATCTCAG
  GATGCCTTGGGACTTAGCAAATTGGGGGGACAGGCTGAAAAAAATCGTAAACTTCAAAAAAAGCATGAAT
  TGCTGGGGGAAGCCCGGAGACAAGGTTTACCTTTTGCCCAATGGGACGGGCCAACGGTTGTGGTCTGGCT
  AGAGCTCTGGGTTGGGATGCCAGCCTGGTATGTGGCTGCCTGCCGAGCAAACGTGAAAAGCGGGGCCATC
  ATGTCGGCCCTGTCCGACACAGAGATCCAGCGTGAGATTGGCATCAGCAACCCCCTGCACAGGCTGAAGC
  TGAGGCTGGCCATCCAGGAGATCATGTCGCTGACCAGCCCGTCTGCCCCGCCCACATCTAGAACGACACT
  CGCCTATGGGGACATGAACCACGAGTGGATCGGCAACGAGTGGCTCCCCAGCCTGGGCCTCCCCCAGTAC
  CGCAGCTACTTCATGGAGTGCCTTGTAGACGCCAGGATGCTGGACCACTTGACCAAGAAAGACCTTCGAG
  GGCAGCTGAAAATGGTCGACAGTTTTCACAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGTT
  AAATTATGACCGGAAAGAACTGGAAAGAAAAAGAGAAGAAAGTCAGAGTGAAATAAAAGACGTGCTTGTT
  TGGAGCAATGATCGAGTGATTCGCTGGATCCTGTCAATTGGCCTTAAAGAATATGCAAACAATCTTATAG
  AGAGTGGTGTTCACGGAGCACTTCTGGCCTTAGATGAAACCTTCGACTTCAGTGCACTGGCACTGCTGTT
  ACAGATCCCAACGCAGAACACACAGGCTCGTGCTGTCTTGGAAAGAGAATTTAACAACCTTTTGGTCATG
  GGGACTGATAGAAGGTTTGATGAAGATGATGATAAAAGCTTTAGGAGAGCACCTTCATGGAGAAAAAAGT
  TTAGACCAAAGGACATTCGTGGCTTAGCTGCTGGGTCAGCAGAGACTCTCCCTGCAAACTTCCGGGTGAC
  TTCTTCTATGTCTTCCCCCTCTATGCAGCCAAAGAAGATGCAGATGGACGGCAATGTATCAGGAACACAG
  AGGTTGGATTCTGCTACAGTCAGGACTTACTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205488 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHE
  VGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLR
  MTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQN
  NQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHV
  TELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEI
  ANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQME
  AQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQL
  EGTQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRTQKGRL
  AALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAM
  MLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRS
  TPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDI
  RNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQ
  DALGLSKLGGQAEKNRKLQKKHELLGEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAI
  MSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQY
  RSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLV
  WSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVM
  GTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQ
  RLDSATVRTYSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003626
• ORF Size: 3606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003626.5
• RefSeq Size: 5353 bp
• RefSeq ORF: 3609 bp
• Locus ID: 8500
• UniProt ID: Q13136
• Cytogenetics: 11q13.3
• Domains: SAM
• Protein Families: Druggable Genome, Phosphatase
• MW: 135.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]