Liprin alpha 1 (PPFIA1) (NM_003626) Human Tagged ORF Clone

CAT#: RC205488

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PPFIA1 (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 (PPFIA1), transcript variant 2



  "NM_003626" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 880.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PPFIA1
Synonyms LIP.1; LIP1; LIPRIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTGCGAGGTGATGCCGACCATCAGCGAAGCAGAAGGCCCCCCTGGAGGAGGTGGAGGCCATGGTT
CCGGCTCCCCTTCACAGCCAGATGCAGATTCACATTTTGAACAGTTGATGGTCTCCATGCTAGAAGAAAG
GGACCGCCTTCTTGATACACTGAGAGAGACTCAAGAAACGCTGGCCTTAACCCAGGGGAAGTTACACGAG
GTTGGTCATGAAAGAGATTCCTTGCAGAGACAGCTCAACACAGCACTTCCACAGGAGTTCGCAGCACTTA
CTAAAGAACTCAATGTATGCAGGGAACAGCTCCTTGAAAGGGAAGAAGAAATTGCTGAACTGAAAGCAGA
AAGGAATAACACCAGGCTGCTGTTAGAGCATTTGGAATGCCTTGTCTCCAGGCATGAGCGGTCTCTTAGG
ATGACCGTGGTGAAGAGACAAGCGCAGTCTCCAGCAGGCGTGTCCAGCGAAGTGGAAGTGCTGAAAGCAC
TGAAGTCCTTATTTGAACACCACAAAGCTCTGGATGAAAAGGTGAGAGAGCGATTACGAGTAGCACTTGA
AAGATGTAGTTTGTTAGAAGAGGAATTAGGTGCCACACACAAAGAGCTAATGATTCTTAAAGAACAGAAT
AATCAGAAAAAAACTCTAACAGATGGAGTGCTGGACATAAACCATGAACAAGAAAATACACCAAGCACGA
GTGGAAAGAGATCTTCTGATGGTTCTTTAAGCCACGAGGAAGACCTTGCTAAAGTAATTGAGCTCCAAGA
AATCATAAGTAAGCAGTCAAGGGAACAGAGCCAAATGAAAGAACGCCTGGCTTCCCTTTCCAGTCATGTG
ACAGAACTGGAAGAGGATCTGGACACGGCTAGAAAAGATCTCATCAAATCTGAAGAAATGAACACAAAAT
TGCAACGAGATGTCCGTGAAGCCATGGCCCAAAAGGAAGATATGGAAGAGAGAATCACTACTCTTGAAAA
ACGCTACCTCGCTGCACAGCGTGAAGCCACATCTGTGCATGACCTCAATGATAAACTTGAAAATGAAATT
GCAAATAAAGATTCTATGCATCGACAGACTGAAGATAAAAACCGCCAGTTACAGGAGCGCTTGGAATTGG
CAGAGCAAAAGCTGCAACAGACACTGAGGAAGGCAGAGACGCTCCCGGAGGTGGAGGCGGAGCTGGCCCA
GAGGGTGGCAGCGCTTTCCAAGGCTGAAGAGAGACACGGCAACATTGAAGAAAGGTTACGACAGATGGAA
GCACAGTTGGAGGAGAAGAATCAAGAACTGCAGCGGGCAAGGCAAAGAGAAAAAATGAACGAAGAACATA
ATAAACGTTTATCAGACACTGTTGACAAGCTGCTTTCAGAATCTAATGAGAGGCTTCAACTTCATCTTAA
AGAGAGAATGGCTGCTTTGGAAGATAAGAACTCTCTTTTAAGAGAAGTTGAAAGTGCAAAAAAGCAGTTA
GAAGGAACACAACACGATAAGGATCAGCTTGTCCTAAACATTGAAGCACTGAGGGCTGAACTAGACCACA
TGAGACTAAGAGGTGCTTCACTTCATCATGGCCGACCCCACTTGGGCAGTGTCCCAGATTTCAGGTTCCC
CATGGCAGATGGCCACACAGACTCCTACAGCACCAGTGCAGTGCTGCGGCGCACACAGAAAGGCCGGCTG
GCAGCCCTGCGAGATGAGCCTTCCAAGGTACAAACTCTTAATGAGCAGGATTGGGAACGTGCCCAGCAAG
CTAGTGTCTTGGCAAATGTAGCACAAGCATTCGAGAGTGATGCTGACGTGTCTGATGGTGAAGATGACAG
GGACACTCTCCTCAGCTCAGTTGACCTGCTATCGCCCAGCGGGCAGGCCGACGCGCACACACTAGCCATG
ATGCTTCAGGAGCAGCTGGACGCCATCAACAAAGAGATCAGGTTGATTCAGGAAGAAAAAGAAAATACAG
AGCAGCGGGCAGAGGAGATTGAAAGTCGAGTTGGCAGTGGAAGTCTAGACAATCTTGGTCGTTTTAGATC
AATGAGCTCCATTCCCCCCTACCCTGCTTCCTCGCTTGCTAGCTCCTCCCCTCCGGGCAGTGGGCGCTCC
ACCCCACGAAGGATCCCTCACAGCCCAGCTCGGGAAGTGGACAGACTGGGCGTCATGACCCTTTTGCCAC
CTTCCAGAGAAGAGGTACGAGATGACAAGACAACCATAAAGTGTGAAACCTCCCCGCCTTCCTCCCCGAG
AGCCCTTCGGTTAGACCGGCTGCACAAAGGGGCGCTGCACACCGTTAGCCACGAGGACATCAGGGACATA
AGGAACTCCACAGGCTCCCAGGATGGTCCCGTGAGCAACCCCAGCAGTAGCAACAGTAGCCAGGACTCGC
TCCACAAAGCCCCAAAGAAGAAAGGCATTAAGTCCTCCATTGGCCGCTTGTTTGGCAAGAAAGAAAAGGG
CCGACCTGGACAAACTGGCAAAGAAGCATTAGGACAAGCTGGTGTTTCCGAGACGGATAACTCATCTCAG
GATGCCTTGGGACTTAGCAAATTGGGGGGACAGGCTGAAAAAAATCGTAAACTTCAAAAAAAGCATGAAT
TGCTGGGGGAAGCCCGGAGACAAGGTTTACCTTTTGCCCAATGGGACGGGCCAACGGTTGTGGTCTGGCT
AGAGCTCTGGGTTGGGATGCCAGCCTGGTATGTGGCTGCCTGCCGAGCAAACGTGAAAAGCGGGGCCATC
ATGTCGGCCCTGTCCGACACAGAGATCCAGCGTGAGATTGGCATCAGCAACCCCCTGCACAGGCTGAAGC
TGAGGCTGGCCATCCAGGAGATCATGTCGCTGACCAGCCCGTCTGCCCCGCCCACATCTAGAACGACACT
CGCCTATGGGGACATGAACCACGAGTGGATCGGCAACGAGTGGCTCCCCAGCCTGGGCCTCCCCCAGTAC
CGCAGCTACTTCATGGAGTGCCTTGTAGACGCCAGGATGCTGGACCACTTGACCAAGAAAGACCTTCGAG
GGCAGCTGAAAATGGTCGACAGTTTTCACAGAAACAGTTTCCAGTGTGGAATTATGTGCCTGAGAAGGTT
AAATTATGACCGGAAAGAACTGGAAAGAAAAAGAGAAGAAAGTCAGAGTGAAATAAAAGACGTGCTTGTT
TGGAGCAATGATCGAGTGATTCGCTGGATCCTGTCAATTGGCCTTAAAGAATATGCAAACAATCTTATAG
AGAGTGGTGTTCACGGAGCACTTCTGGCCTTAGATGAAACCTTCGACTTCAGTGCACTGGCACTGCTGTT
ACAGATCCCAACGCAGAACACACAGGCTCGTGCTGTCTTGGAAAGAGAATTTAACAACCTTTTGGTCATG
GGGACTGATAGAAGGTTTGATGAAGATGATGATAAAAGCTTTAGGAGAGCACCTTCATGGAGAAAAAAGT
TTAGACCAAAGGACATTCGTGGCTTAGCTGCTGGGTCAGCAGAGACTCTCCCTGCAAACTTCCGGGTGAC
TTCTTCTATGTCTTCCCCCTCTATGCAGCCAAAGAAGATGCAGATGGACGGCAATGTATCAGGAACACAG
AGGTTGGATTCTGCTACAGTCAGGACTTACTCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205488 protein sequence
Red=Cloning site Green=Tags(s)

MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQETLALTQGKLHE
VGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLR
MTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGATHKELMILKEQN
NQKKTLTDGVLDINHEQENTPSTSGKRSSDGSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHV
TELEEDLDTARKDLIKSEEMNTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEI
ANKDSMHRQTEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQME
AQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLLREVESAKKQL
EGTQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMADGHTDSYSTSAVLRRTQKGRL
AALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADVSDGEDDRDTLLSSVDLLSPSGQADAHTLAM
MLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRS
TPRRIPHSPAREVDRLGVMTLLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDI
RNSTGSQDGPVSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQ
DALGLSKLGGQAEKNRKLQKKHELLGEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAI
MSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQY
RSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQSEIKDVLV
WSNDRVIRWILSIGLKEYANNLIESGVHGALLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVM
GTDRRFDEDDDKSFRRAPSWRKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQ
RLDSATVRTYSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003626
ORF Size 3606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003626.5
RefSeq Size 5353 bp
RefSeq ORF 3609 bp
Locus ID 8500
UniProt ID Q13136
Cytogenetics 11q13.3
Domains SAM
Protein Families Druggable Genome, Phosphatase
MW 135.6 kDa
Gene Summary The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. This protein binds to the intracellular membrane-distal phosphatase domain of tyrosine phosphatase LAR, and appears to localize LAR to cell focal adhesions. This interaction may regulate the disassembly of focal adhesion and thus help orchestrate cell-matrix interactions. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

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