WDR44 (NM_019045) Human Tagged ORF Clone

CAT#: RC205485

WDR44 (Myc-DDK-tagged)-Human WD repeat domain 44 (WDR44), transcript variant 1

Product Images

Circular map for RC205485

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  Western blot validation of overexpression lysate (Cat# LY402730) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205485 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDR44 protein (Cat# TP305485). The protein was produced from HEK293T cells transfected with WDR44 cDNA clone (Cat# RC205485) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR44
• Synonyms: RAB11BP; RPH11; SYM-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205485 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGAAAGCGACACCGAGGAATTCTATGATGCCCCTGAAGATGTGCACCTAGGGGGCGGCTACC
  CCGTGGGGTCTCCAGGAAAAGTTGGGCTTTCAACATTCAAGGAAACAGAGAACACTGCATACAAAGTTGG
  AAATGAGTCCCCTGTACAAGAATTGAAACAAGATGTGTCTAAAAAGATTATTGAAAGTATTATTGAGGAG
  AGTCAGAAAGTACTACAGCTTGAAGATGACTCTTTGGATTCCAAAGGAAAAGAACTCTCTGATCAAGCTA
  CTGCCAGTCCTATTGTGGCTAGAACAGATCTGAGCAATATACCCGGACTGTTAGCCATAGATCAAGTACT
  ACCGGAAGAATCCCAAAAGGCAGAGAGTCAGAATACATTTGAAGAGACTGAATTAGAATTAAAAAAATGC
  TTTCCTTCTGATGAAACCTGTGAGAAACCAGTAGATGAAACCACGAAGTTAACTCAAACAAGTTCAACTG
  AGCAGCTTAATGTGCTTGAAACTGAAACAGAAGTATTGAACAAGGAAGCAGTGGAAGTCAAAGGAGGTGG
  TGATGTTTTAGAGCCTGTGCCCTCAGACTCCTTATCTACTAAAGATTTTGCCGCTGTGGAAGAAGTGGCC
  CCTGCCAAACCCCCAAGACACCTTACTCCAGAGCCTGATATAGTGGCTAGTACAAAGAAGCCTGTTCCAG
  CACGCCCACCTCCTCCAACTAATTTCCCACCTCCTAGACCCCCACCTCCTTCTCGACCTGCTCCACCACC
  AAGAAAAAGGAAAAGCGAATTGGAATTTGAGACTCTGAAAACTCCTGATATAGATGTTCCCAAAGAGAAT
  ATTACGTCTGATTCTCTCCTAACCGCAAGCATGGCTTCAGAAAGTGCGGTTAAGGATTCTCAGCCTTCTC
  TTGATTTGGCAAGTGCTACCAGTGGAGATAAAATAGTTACCGCCCAGGAAAATGGAAAAGCACCTGATGG
  GCAGACTGTAGCAGGTGAAGTGATGGGCCCTCAGAGACCTAGATCCAACTCTGGGAGAGAGCTTACTGAT
  GAGGAAATTTTAGCCAGTGTAATGATTAAGAACCTGGATACTGGAGAAGAAATACCTTTGAGTCTTGCAG
  AAGAGAAACTACCAACAGGCATTAATCCTCTCACTCTACACATCATGAGAAGGACAAAAGAATATGTAAG
  TAATGACGCGGCACAGTCAGATGATGAAGAGAAGTTACAGTCTCAGCCAACAGATACTGATGGTGGAAGG
  TTAAAACAGAAAACGACTCAACTAAAGAAGTTTCTTGGAAAATCAGTAAAGAGAGCAAAGCACCTTGCTG
  AGGAATATGGTGAACGTGCTATAAATAAAGTTAAAAGTGTTAGAGATGAAGTGTTTCATACTGATCAAGA
  TGATCCTTCATCAAGTGATGATGAAGGAATGCCATACACAAGACCAGTTAAATTCAAAGCAGCACACGGT
  TTTAAAGGACCTTATGATTTTGATCAGATCAAAGTGGTGCAAGATCTTAGTGGTGAACATATGGGAGCTG
  TTTGGACCATGAAATTTTCTCACTGTGGCCGATTACTTGCCTCAGCTGGACAAGACAATGTAGTGAGAAT
  ATGGGCTTTAAAAAATGCTTTTGACTATTTCAACAATATGCGAATGAAATACAATACTGAAGGACGTGTG
  TCCCCATCACCCTCTCAGGAAAGTCTAAGTTCATCAAAATCGGATACAGATACAGGGGTATGCAGTGGAA
  CTGATGAAGACCCTGATGATAAAAACGCACCCTTTCGGCAACGGCCATTTTGCAAATATAAAGGACATAC
  TGCTGATCTCCTTGATCTTTCATGGTCTAAAAACTACTTTCTTCTTTCTTCTTCAATGGATAAAACAGTC
  AGATTATGGCACATTTCTCGAAGAGAATGCCTTTGCTGTTTTCAACATATAGATTTTGTCACTGCCATAG
  CTTTTCATCCAAGAGATGACAGGTATTTTCTAAGTGGGTCTTTGGATGGAAAGCTCCGCCTTTGGAACAT
  ACCTGACAAAAAAGTGGCTTTGTGGAATGAAGTAGATGGTCAGACAAAATTGATCACAGCTGCAAATTTC
  TGTCAGAATGGCAAATATGCAGTGATTGGGACATATGATGGCAGATGTATTTTCTATGATACAGAGCATT
  TGAAATACCATACACAAATACATGTCCGATCTACTAGAGGGCGCAACAAGGTTGGAAGAAAAATTACTGG
  CATTGAGCCTTTACCTGGAGAAAATAAGATATTGGTAACCTCAAATGACTCCAGAATCAGACTATATGAT
  TTGAGAGATTTGTCACTATCCATGAAGTATAAGGGTTACGTCAATAGCAGCAGCCAGATCAAAGCAAGTT
  TCAGCCATGATTTTACTTACCTCGTTAGTGGTTCAGAAGATAAGTATGTTTATATCTGGAGTACCTACCA
  TGACCTAAGCAAGTTTACTTCAGTCAGGAGAGATCGTAATGACTTCTGGGAAGGTATTAAAGCCCACAAT
  GCAGTTGTTACATCAGCCATCTTTGCACCAAACCCAAGTTTGATGTTATCTTTGGATGTGCAATCTGAAA
  AATCAGAAGGGAACGAGAAAAGTGAAGATGCTGAAGTTTTGGATGCCACACCTTCTGGTATTATGAAGAC
  AGATAACACAGAAGTTCTTCTCTCTGCTGACTTCACTGGAGCAATCAAAGTGTTTGTTAATAAAAGAAAA
  AATGTATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205485 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASESDTEEFYDAPEDVHLGGGYPVGSPGKVGLSTFKETENTAYKVGNESPVQELKQDVSKKIIESIIEE
  SQKVLQLEDDSLDSKGKELSDQATASPIVARTDLSNIPGLLAIDQVLPEESQKAESQNTFEETELELKKC
  FPSDETCEKPVDETTKLTQTSSTEQLNVLETETEVLNKEAVEVKGGGDVLEPVPSDSLSTKDFAAVEEVA
  PAKPPRHLTPEPDIVASTKKPVPARPPPPTNFPPPRPPPPSRPAPPPRKRKSELEFETLKTPDIDVPKEN
  ITSDSLLTASMASESAVKDSQPSLDLASATSGDKIVTAQENGKAPDGQTVAGEVMGPQRPRSNSGRELTD
  EEILASVMIKNLDTGEEIPLSLAEEKLPTGINPLTLHIMRRTKEYVSNDAAQSDDEEKLQSQPTDTDGGR
  LKQKTTQLKKFLGKSVKRAKHLAEEYGERAINKVKSVRDEVFHTDQDDPSSSDDEGMPYTRPVKFKAAHG
  FKGPYDFDQIKVVQDLSGEHMGAVWTMKFSHCGRLLASAGQDNVVRIWALKNAFDYFNNMRMKYNTEGRV
  SPSPSQESLSSSKSDTDTGVCSGTDEDPDDKNAPFRQRPFCKYKGHTADLLDLSWSKNYFLLSSSMDKTV
  RLWHISRRECLCCFQHIDFVTAIAFHPRDDRYFLSGSLDGKLRLWNIPDKKVALWNEVDGQTKLITAANF
  CQNGKYAVIGTYDGRCIFYDTEHLKYHTQIHVRSTRGRNKVGRKITGIEPLPGENKILVTSNDSRIRLYD
  LRDLSLSMKYKGYVNSSSQIKASFSHDFTYLVSGSEDKYVYIWSTYHDLSKFTSVRRDRNDFWEGIKAHN
  AVVTSAIFAPNPSLMLSLDVQSEKSEGNEKSEDAEVLDATPSGIMKTDNTEVLLSADFTGAIKVFVNKRK
  NVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019045
• ORF Size: 2739 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019045.5
• RefSeq Size: 4160 bp
• RefSeq ORF: 2742 bp
• Locus ID: 54521
• UniProt ID: Q5JSH3
• Cytogenetics: Xq24
• Domains: WD40
• MW: 101.3 kDa
• Gene Summary: This gene encodes a protein that interacts with the small GTPase rab11. A similar protein in rat binds the GTP-containing active form of rab11. This protein may play a role in endosome recycling. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]