LRRC50 (DNAAF1) (NM_178452) Human Tagged ORF Clone

CAT#: RC205452

DNAAF1 (Myc-DDK-tagged)-Human leucine rich repeat containing 50 (LRRC50)

Product Images

Circular map for RC205452

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LRRC50 (Cat# RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50(Cat# TA504546). Positive lysates LY405926 (100ug) and LC405926 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY405926) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205452 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DNAAF1 protein (Cat# TP305452). The protein was produced from HEK293T cells transfected with DNAAF1 cDNA clone (Cat# RC205452) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC50
• Synonyms: CILD13; DAU1; LRRC50; ODA7; swt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205452 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCCTGAGCCCTCGGAGCCTGCGACAGGTGGTGCAGCAGAGCTGGATTGCGCGCAGGAGCCCGGCG
  TGGAGGAGTCTGCGGGTGACCACGGGAGCGCAGGCCGAGGGGGCTGCAAGGAAGAAATTAATGATCCTAA
  GGAAATATGTGTGGGTTCTTCTGACACATCCTACCACAGCCAGCAGAAACAGAGTGGTGATAATGGGTCA
  GGTGGTCACTTCGCACACCCAAGAGAAGACAGGGAAGATCGGGGCCCCAGAATGACTAAAAGTTCCCTGC
  AAAAACTCTGCAAGCAGCACAAGCTTTATATTACCCCAGCATTGAATGATACGCTGTATTTACACTTTAA
  AGGTTTTGATCGCATTGAGAACCTGGAAGAGTACACAGGGCTGCGCTGTCTCTGGCTGCAGAGCAATGGA
  ATACAGAAAATCGAAAACCTGGAGGCCCAAACTGAGTTGCGTTGCCTCTTCTTGCAAATGAACTTGCTCC
  GTAAAATTGAGAACCTGGAACCTCTGCAGAAACTGGATGCTCTTAACCTCAGCAACAATTACATCAAGAC
  CATTGAAAACCTCTCCTGCCTCCCAGTCCTGAACACATTGCAGATGGCCCACAATCACCTGGAGACCGTG
  GAGGACATTCAGCATCTACAAGAGTGTTTGAGGCTTTGTGTCCTTGACCTTTCGCACAACAAGCTGAGTG
  ACCCGGAGATCCTGAGCATTCTGGAAAGCATGCCCGATTTGCGTGTACTGAATTTGATGGGAAACCCGGT
  TATCAGACAGATTCCTAATTACAGAAGGACAGTCACTGTACGACTAAAGCACTTAACATACCTGGATGAT
  AGACCAGTGTTTCCAAAGGACAGAGCTTGTGCGGAGGCCTGGGCTAGGGGAGGGTACGCAGCTGAAAAGG
  AGGAGAGACAGCAGTGGGAGAGCAGGGAGCGGAAGAAGATCACAGACAGCATTGAAGCCTTGGCCATGAT
  CAAGCAGCGGGCAGAGGAGAGGAAAAGACAGAGAGAGAGTCAAGAGAGAGGGGAGATGACATCTTCAGAT
  GATGGTGAGAATGTGCCCGCCAGTGCGGAAGGCAAGGAGGAGCCTCCCGGGGACAGAGAAACAAGGCAGA
  AGATGGAGCTATTTGTTAAGGAAAGCTTTGAGGCCAAGGACGAGCTCTGCCCGGAAAGGCCAAGTGGAGA
  GGAGCCGCCTGTGGAGGCTAAAAGAGAGGATGGAGGTCCAGAGCCAGAGGGGACCCTCCCAGCTGAGACC
  CTGCTACTGTCGTCACCTGTGGAGGTTAAAGGAGAGGACGGAGATGGAGAGCCAGAGGGGACCCTCCCAG
  CTGAGGCCCCACCACCCCCGCCACCTGTGGAGGTTAAAGGAGAGGATGGAGATCAAGAGCCAGAGGGGAC
  CCTCCCAGCTGAGACCCTGCTACTGTCACCGCCTGTGAAGGTTAAAGGAGAGGATGGAGATCGAGAGCCA
  GAGGGGACCCTCCCAGCTGAGGCCCCACCACCACTGCCCCTGGGAGCTGCCAGGGAAGAACCGACTCCCC
  AGGCTGTGGCCACTGAGGGTGTATTCGTTACAGAACTTGATGGAACGAGAACGGAAGATTTAGAAACCAT
  TAGACTGGAGACAAAGGAGACATGCTGCATTGATGACCTACCTGACTTGGAAGATGATGATGAAACAGGC
  AAATCTCTGGAAGACCAGAATATGTGCTTTCCGAAGATTGAGGTCATCTCGAGCTTGAGTGATGACAGTG
  ACCCTGAACTGGACTACACGTCACTCCCTGTGCTGGAAAACCTGCCCACAGACACTCTGTCAAATATATT
  TGCAGTCTCTAAAGACACCTCAAAGGCGGCTCGGGTGCCCTTCACAGACATCTTTAAAAAAGAAGCTAAG
  AGGGACTCGGAAATCCGAAAACAAGACACCAAGTCCCCAAGACCCCTGATCCAGGAGCTCAGCGACGAGG
  ACCCCTCTGGCCAGCCACTGATGCCCCCCACCTGCCAAAGAGATGCTGCACCACTCACTTCCACTGGAGA
  CAGGGACAGCGACTTCCTTGCAGCCTCTTCTCCGGTGCCGACTGAGAGCGCCGCCACACCCCCAGAGACG
  TGTGTCGGAGTTGCCCAGCCCAGCCAAGCTCTGCCCACGTGGGACCTCACTGCATTCCCAGCACCGAAAG
  CATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPEPSEPATGGAAELDCAQEPGVEESAGDHGSAGRGGCKEEINDPKEICVGSSDTSYHSQQKQSGDNGS
  GGHFAHPREDREDRGPRMTKSSLQKLCKQHKLYITPALNDTLYLHFKGFDRIENLEEYTGLRCLWLQSNG
  IQKIENLEAQTELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNHLETV
  EDIQHLQECLRLCVLDLSHNKLSDPEILSILESMPDLRVLNLMGNPVIRQIPNYRRTVTVRLKHLTYLDD
  RPVFPKDRACAEAWARGGYAAEKEERQQWESRERKKITDSIEALAMIKQRAEERKRQRESQERGEMTSSD
  DGENVPASAEGKEEPPGDRETRQKMELFVKESFEAKDELCPERPSGEEPPVEAKREDGGPEPEGTLPAET
  LLLSSPVEVKGEDGDGEPEGTLPAEAPPPPPPVEVKGEDGDQEPEGTLPAETLLLSPPVKVKGEDGDREP
  EGTLPAEAPPPLPLGAAREEPTPQAVATEGVFVTELDGTRTEDLETIRLETKETCCIDDLPDLEDDDETG
  KSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAK
  RDSEIRKQDTKSPRPLIQELSDEDPSGQPLMPPTCQRDAAPLTSTGDRDSDFLAASSPVPTESAATPPET
  CVGVAQPSQALPTWDLTAFPAPKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178452
• ORF Size: 2175 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178452.3, NP_848547.3
• RefSeq Size: 2451 bp
• RefSeq ORF: 2178 bp
• Locus ID: 123872
• UniProt ID: Q8NEP3
• Cytogenetics: 16q24.1
• MW: 80 kDa
• Gene Summary: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]