KPNA4 (NM_002268) Human Tagged ORF Clone

CAT#: RC205448

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KPNA4 (Myc-DDK-tagged)-Human karyopherin alpha 4 (importin alpha 3) (KPNA4)



  "NM_002268" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KPNA4
Synonyms IPOA3; QIP1; SRP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205448 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACAACGAGAAACTGGACAACCAACGGCTCAAGAATTTCAAGAACAAAGGCCGCGACTTGGAGA
CTATGAGAAGACAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAAAGAGATGAACATCTCTTAAA
GAGAAGGAATGTACCACATGAAGATATCTGTGAAGACTCTGATATAGATGGTGATTATAGAGTGCAAAAT
ACCTCTCTAGAAGCTATTGTTCAAAATGCTTCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAG
CTGCTAGGAAGCTTTTGTCCAGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCC
CATTTTAGTCCATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTGAAGCTGCATGGGCTTTGACA
AACATTGCATCTGGAACTTCTGAACAAACTCAAGCAGTAGTTCAGTCCAATGCTGTGCCACTTTTCCTGA
GGCTTCTCCATTCACCCCATCAGAATGTCTGTGAGCAAGCAGTGTGGGCATTGGGAAATATCATAGGTGA
TGGGCCCCAGTGTAGAGATTATGTCATAAGTCTTGGAGTTGTGAAACCTTTACTTTCCTTCATAAGTCCA
TCTATTCCTATAACATTCTTAAGAAATGTTACTTGGGTTATGGTCAACTTATGTCGCCACAAAGACCCAC
CACCACCAATGGAAACCATTCAGGAGATTCTTCCAGCCCTTTGTGTTTTAATTCATCACACAGATGTAAA
TATACTGGTAGACACAGTCTGGGCCCTCTCTTACCTTACTGATGCTGGCAATGAACAAATACAGATGGTA
ATAGACTCTGGAATAGTTCCTCATTTGGTTCCTCTGCTCAGCCACCAGGAAGTTAAAGTTCAGACTGCTG
CACTTAGAGCTGTGGGCAACATTGTTACTGGAACTGATGAGCAAACACAAGTAGTTTTGAACTGTGATGC
TCTTTCACACTTCCCAGCACTCCTGACACATCCCAAAGAGAAAATTAATAAAGAAGCAGTGTGGTTCCTC
TCCAACATCACTGCAGGAAATCAGCAGCAGGTACAGGCAGTAATTGATGCCAATCTTGTACCAATGATAA
TACACCTTTTGGATAAGGGGGATTTTGGCACTCAAAAAGAAGCTGCTTGGGCCATAAGTAACTTAACAAT
TAGTGGAAGGAAAGATCAAGTGGCTTACCTTATCCAACAAAATGTTATCCCACCTTTTTGCAACTTGCTG
ACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTCGATGGACTAAGTAATATATTAAAAATGGCTGAAG
ATGAGGCAGAAACCATAGGCAATCTTATAGAAGAATGTGGAGGGCTGGAGAAAATTGAACAACTTCAAAA
TCATGAAAATGAAGACATCTACAAATTGGCCTATGAGATCATTGATCAGTTCTTCTCTTCAGATGATATT
GATGAAGACCCTAGCCTTGTTCCAGAGGCAATTCAAGGCGGAACATTTGGTTTCAATTCATCTGCCAATG
TACCAACAGAAGGGTTCCAGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205448 protein sequence
Red=Cloning site Green=Tags(s)

MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQN
TSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALT
NIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISP
SIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMV
IDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL
SNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNLL
TVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDI
DEDPSLVPEAIQGGTFGFNSSANVPTEGFQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002268
ORF Size 1563 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002268.5
RefSeq Size 8981 bp
RefSeq ORF 1566 bp
Locus ID 3840
UniProt ID O00629
Cytogenetics 3q25.33
Domains Armadillo_seg, IBB
MW 57.9 kDa
Gene Summary The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]

Other Versions

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