KPNA4 (NM_002268) Human Tagged ORF Clone

CAT#: RC205448

KPNA4 (Myc-DDK-tagged)-Human karyopherin alpha 4 (importin alpha 3) (KPNA4)

Product Images

Circular map for RC205448

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  Western blot validation of overexpression lysate (Cat# LY400822) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205448 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KPNA4 protein (Cat# TP305448). The protein was produced from HEK293T cells transfected with KPNA4 cDNA clone (Cat# RC205448) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KPNA4
• Synonyms: IPOA3; QIP1; SRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205448 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACAACGAGAAACTGGACAACCAACGGCTCAAGAATTTCAAGAACAAAGGCCGCGACTTGGAGA
  CTATGAGAAGACAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAAAGAGATGAACATCTCTTAAA
  GAGAAGGAATGTACCACATGAAGATATCTGTGAAGACTCTGATATAGATGGTGATTATAGAGTGCAAAAT
  ACCTCTCTAGAAGCTATTGTTCAAAATGCTTCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAG
  CTGCTAGGAAGCTTTTGTCCAGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCC
  CATTTTAGTCCATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTGAAGCTGCATGGGCTTTGACA
  AACATTGCATCTGGAACTTCTGAACAAACTCAAGCAGTAGTTCAGTCCAATGCTGTGCCACTTTTCCTGA
  GGCTTCTCCATTCACCCCATCAGAATGTCTGTGAGCAAGCAGTGTGGGCATTGGGAAATATCATAGGTGA
  TGGGCCCCAGTGTAGAGATTATGTCATAAGTCTTGGAGTTGTGAAACCTTTACTTTCCTTCATAAGTCCA
  TCTATTCCTATAACATTCTTAAGAAATGTTACTTGGGTTATGGTCAACTTATGTCGCCACAAAGACCCAC
  CACCACCAATGGAAACCATTCAGGAGATTCTTCCAGCCCTTTGTGTTTTAATTCATCACACAGATGTAAA
  TATACTGGTAGACACAGTCTGGGCCCTCTCTTACCTTACTGATGCTGGCAATGAACAAATACAGATGGTA
  ATAGACTCTGGAATAGTTCCTCATTTGGTTCCTCTGCTCAGCCACCAGGAAGTTAAAGTTCAGACTGCTG
  CACTTAGAGCTGTGGGCAACATTGTTACTGGAACTGATGAGCAAACACAAGTAGTTTTGAACTGTGATGC
  TCTTTCACACTTCCCAGCACTCCTGACACATCCCAAAGAGAAAATTAATAAAGAAGCAGTGTGGTTCCTC
  TCCAACATCACTGCAGGAAATCAGCAGCAGGTACAGGCAGTAATTGATGCCAATCTTGTACCAATGATAA
  TACACCTTTTGGATAAGGGGGATTTTGGCACTCAAAAAGAAGCTGCTTGGGCCATAAGTAACTTAACAAT
  TAGTGGAAGGAAAGATCAAGTGGCTTACCTTATCCAACAAAATGTTATCCCACCTTTTTGCAACTTGCTG
  ACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTCGATGGACTAAGTAATATATTAAAAATGGCTGAAG
  ATGAGGCAGAAACCATAGGCAATCTTATAGAAGAATGTGGAGGGCTGGAGAAAATTGAACAACTTCAAAA
  TCATGAAAATGAAGACATCTACAAATTGGCCTATGAGATCATTGATCAGTTCTTCTCTTCAGATGATATT
  GATGAAGACCCTAGCCTTGTTCCAGAGGCAATTCAAGGCGGAACATTTGGTTTCAATTCATCTGCCAATG
  TACCAACAGAAGGGTTCCAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205448 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQN
  TSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALT
  NIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISP
  SIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMV
  IDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL
  SNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNLL
  TVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDI
  DEDPSLVPEAIQGGTFGFNSSANVPTEGFQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002268
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002268.5
• RefSeq Size: 8981 bp
• RefSeq ORF: 1566 bp
• Locus ID: 3840
• UniProt ID: O00629
• Cytogenetics: 3q25.33
• Domains: Armadillo_seg, IBB
• MW: 57.9 kDa
• Gene Summary: The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]