DPP10 (NM_020868) Human Tagged ORF Clone

CAT#: RC205435

DPP10 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 10 (non-functional) (DPP10), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DPP10 (Cat# RC205435, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPP10 (Cat# TA503585). Positive lysates LY412236 (100ug) and LC412236 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412236) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205435 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DPP10 protein (Cat# TP305435). The protein was produced from HEK293T cells transfected with DPP10 cDNA clone (Cat# RC205435) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells transfected with either RC205435 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DPP10 antibody (TA503585), and then analyzed by flow cytometry.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPP10
• Synonyms: DPL2; DPPY; DPRP-3; DPRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205435 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCAAACTGCCAGCGTGTCCCATCACATCAAGTGTCAACCCTCAAAAACAATCAAGGAACTGGGAA
  GTAACAGCCCTCCACAGAGAAACTGGAAGGGAATTGCTATTGCTCTGCTGGTGATTTTAGTTGTATGCTC
  ACTCATCACTATGTCAGTCATCCTCTTAACCCCAGATGAACTCACAAATTCGTCAGAAACCAGATTGTCT
  TTGGAAGACCTCTTTAGGAAAGACTTTGTGCTTCACGATCCAGAGGCTCGGTGGATCAATGATACAGATG
  TGGTGTATAAAAGCGAGAATGGACATGTCATTAAACTGAATATAGAAACAAATGCTACCACATTATTATT
  GGAAAACACAACTTTTGTAACCTTCAAAGCATCAAGACATTCAGTTTCACCAGATTTAAAATATGTCCTT
  CTGGCATATGATGTCAAACAGATTTTTCATTATTCATATACTGCTTCATATGTGATTTACAACATACACA
  CTAGGGAAGTTTGGGAGTTAAATCCTCCAGAAGTAGAGGACTCCGTCTTGCAGTACGCGGCCTGGGGTGT
  CCAAGGGCAGCAGCTGATTTATATTTTTGAAAATAATATCTACTATCAACCTGATATAAAGAGCAGTTCA
  TTGCGACTGACATCTTCTGGAAAAGAAGAAATAATTTTTAATGGGATTGCTGACTGGTTATATGAAGAGG
  AACTCCTGCATTCTCACATCGCCCACTGGTGGTCACCAGATGGAGAAAGACTTGCCTTCCTGATGATAAA
  TGACTCTTTGGTACCCACCATGGTTATCCCTCGGTTTACTGGAGCGTTGTATCCCAAAGGAAAGCAGTAT
  CCGTATCCTAAGGCAGGTCAAATGAACCCAACAATAAAATTATATGTTGTAAACCTGTATGGACCAACTC
  ACACTTTGGAGCTCATGCCACCTGACAGCTTTAAATCAAGAGAATACTATATCACTATGGTTAAATGGGT
  AAGCAATACCAAGACTGTGGTAAGATGGTTAAACCGAGCTCAGAACATCTCCATCCTCACAGTCTGTGAG
  ACCACTACAGGTGCTTGTAGTAAAAAATATGAGATGACATCAGATACGTGGCTCTCTCAGCAGAATGAGG
  AGCCCGTGTTTTCTAGAGACGGCAGCAAATTCTTTATGACAGTGCCTGTTAAGCAAGGGGGACGTGGAGA
  ATTTCACCACATAGCTATGTTCCTCATCCAGAGTAAAAGTGAGCAAATTACCGTGCGGCATCTGACATCA
  GGAAACTGGGAAGTGATAAAGATCTTGGCATACGATGAAACTACTCAAAAAATTTACTTTCTGAGCACTG
  AATCTTCTCCCAGAGGAAGGCAGCTGTACAGTGCTTCTACTGAAGGATTATTGAATCGCCAATGCATTTC
  ATGTAATTTCATGAAAGAACAATGTACATATTTTGATGCCAGTTTTAGTCCCATGAATCAACATTTCTTA
  TTATTCTGTGAAGGTCCAAGGGTCCCAGTGGTCAGCCTACATAGTACGGACAACCCAGCAAAATATTTTA
  TATTGGAAAGCAATTCTATGCTGAAGGAAGCTATCCTGAAGAAGAAGATAGGAAAGCCAGAAATTAAAAT
  CCTTCATATTGACGACTATGAACTTCCTTTACAGTTGTCCCTTCCCAAAGATTTTATGGACCGAAACCAG
  TATGCTCTTCTGTTAATAATGGATGAAGAACCAGGAGGCCAGCTGGTTACAGATAAGTTCCATATTGACT
  GGGATTCCGTACTCATTGACATGGATAATGTCATTGTAGCAAGATTTGATGGCAGAGGAAGTGGATTCCA
  GGGTCTGAAAATTTTGCAGGAGATTCATCGAAGATTAGGTTCAGTAGAAGTAAAGGACCAAATAACAGCT
  GTGAAATTTTTGCTGAAACTGCCTTACATTGACTCCAAAAGATTAAGCATTTTTGGAAAGGGTTATGGTG
  GCTATATTGCATCAATGATCTTAAAATCAGATGAAAAGCTTTTTAAATGTGGATCCGTGGTTGCACCTAT
  CACAGACTTGAAATTGTATGCCTCAGCTTTCTCTGAAAGATACCTTGGGATGCCATCTAAGGAAGAAAGC
  ACTTACCAGGCAGCCAGTGTGCTACATAATGTTCATGGCTTGAAAGAAGAAAATATATTAATAATTCATG
  GAACTGCTGACACAAAAGTTCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAGCTGGAGT
  GAATTATACTATGCAGGTCTACCCAGATGAAGGTCATAACGTATCTGAGAAGAGCAAGTATCATCTCTAC
  AGCACAATCCTCAAATTCTTCAGTGATTGTTTGAAGGAAGAAATATCTGTGCTACCACAGGAACCAGAAG
  AAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205435 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNQTASVSHHIKCQPSKTIKELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSETRLS
  LEDLFRKDFVLHDPEARWINDTDVVYKSENGHVIKLNIETNATTLLLENTTFVTFKASRHSVSPDLKYVL
  LAYDVKQIFHYSYTASYVIYNIHTREVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSSS
  LRLTSSGKEEIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPTMVIPRFTGALYPKGKQY
  PYPKAGQMNPTIKLYVVNLYGPTHTLELMPPDSFKSREYYITMVKWVSNTKTVVRWLNRAQNISILTVCE
  TTTGACSKKYEMTSDTWLSQQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLIQSKSEQITVRHLTS
  GNWEVIKILAYDETTQKIYFLSTESSPRGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFL
  LFCEGPRVPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQ
  YALLLIMDEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITA
  VKFLLKLPYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEES
  TYQAASVLHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLY
  STILKFFSDCLKEEISVLPQEPEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020868
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020868.1
• RefSeq Size: 5253 bp
• RefSeq ORF: 2391 bp
• Locus ID: 57628
• UniProt ID: Q8N608
• Cytogenetics: 2q14.1
• Domains: Peptidase_S9, DPPIV_N_term
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 90.9 kDa
• Gene Summary: This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]