Chromogranin C (SCG2) (NM_003469) Human Tagged ORF Clone

CAT#: RC205382

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SCG2 (Myc-DDK-tagged)-Human secretogranin II (SCG2)



  "NM_003469" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 861.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SCG2
Synonyms CHGC; EM66; SgII; SN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205382 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAAGCAAAGACCCACTGGCTTGGAGCAGCCCTGTCTCTTATCCCTTTAATTTTCCTCATCTCTG
GGGCTGAAGCAGCTTCATTTCAGAGAAACCAGCTGCTTCAGAAAGAACCAGACCTCAGGTTGGAAAATGT
CCAAAAGTTTCCCAGTCCTGAAATGATCAGGGCTTTGGAGTACATAGAAAACCTCCGACAACAAGCTCAT
AAGGAAGAAAGCAGCCCAGATTATAATCCCTACCAAGGTGTCTCTGTCCCCCTTCAGCAAAAAGAAAATG
GCGATGAAAGCCACTTGCCCGAGAGGGATTCACTGAGTGAAGAAGACTGGATGAGAATAATACTCGAAGC
TTTGAGACAGGCTGAAAATGAGCCTCAGTCTGCACCAAAAGAAAATAAGCCCTATGCCTTGAATTCAGAA
AAGAACTTTCCAATGGACATGAGTGATGATTATGAGACACAGCAGTGGCCAGAAAGAAAGCTTAAGCACA
TGCAATTCCCTCCTATGTATGAAGAGAATTCCAGGGATAACCCCTTTAAACGCACAAATGAAATAGTGGA
GGAACAATATACTCCTCAAAGCCTTGCTACATTGGAATCTGTCTTCCAAGAGCTGGGGAAACTGACAGGA
CCAAACAACCAGAAACGTGAGAGGATGGATGAGGAGCAAAAACTTTATACGGATGATGAAGATGATATCT
ACAAGGCTAATAACATTGCCTATGAAGATGTGGTCGGGGGAGAAGACTGGAACCCAGTAGAGGAGAAAAT
AGAGAGTCAAACCCAGGAAGAGGTGAGAGACAGCAAAGAGAATATAGAAAAAAATGAACAAATCAATGAT
GAGATGAAACGCTCAGGGCAGCTTGGCATCCAGGAAGAAGGTCTTCGGAAAGAGAGTAAAGACCAACTCT
CAGATGATGTCTCCAAAGTAATTGCCTATTTGAAAAGGTTAGTAAATGCTGCAGGAAGTGGGAGGTTACA
GAATGGGCAAAATGGGGAAAGGGCCACCAGGCTTTTTGAGAAACCTCTTGATTCTCAGTCTATTTATCAG
CTGATTGAAATCTCAAGGAATTTACAGATACCCCCAGAAGACTTAATTGAGATGCTCAAAACTGGGGAGA
AGCCGAATGGATCAGTGGAACCGGAGCGGGAGCTTGACCTTCCTGTTGACCTAGATGACATCTCAGAGGC
TGACTTAGACCATCCAGACCTGTTCCAAAATAGGATGCTCTCCAAGAGTGGCTACCCTAAAACACCTGGT
GGTGCTGGGACTGAGGCCCTACCAGACGGGCTCAGTGTTGAGGATATTTTAAATCTTTTAGGGATGGAGA
GTGCAGCAAATCAGAAAACGTCGTATTTTCCCAATCCATATAACCAGGAGAAAGTTCTGCCAAGGCTCCC
TTATGGTGCTGGAAGATCTAGATCGAACCAGCTTCCCAAAGCTGCCTGGATTCCACATGTTGAAAACAGA
CAGATGGCATATGAAAACCTGAACGACAAGGATCAAGAATTAGGTGAGTACTTGGCCAGGATGCTAGTTA
AATACCCTGAGATCATTAATTCAAACCAAGTGAAGCGAGTTCCTGGTCAAGGCTCATCTGAAGGTGACCT
GCAGGAAGAGGAACAAATTGAGCAGGCCATCAAAGAGCATTTGAATCAAGGCAGCTCTCAGGAGACTGAC
AAGCTGGCCCCGGTGAGCAAAAGGTTCCCTGTGGGGCCCCCGAAGAATGATGATACCCCAAATAGGCAGT
ACTGGGATGAAGATCTGTTAATGAAAGTGCTGGAATACCTCAACCAAGAAAAGGCAGAAAAGGGAAGGGA
GCATATTGCTAAGAGAGCAATGGAAAATATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205382 protein sequence
Red=Cloning site Green=Tags(s)

MAEAKTHWLGAALSLIPLIFLISGAEAASFQRNQLLQKEPDLRLENVQKFPSPEMIRALEYIENLRQQAH
KEESSPDYNPYQGVSVPLQQKENGDESHLPERDSLSEEDWMRIILEALRQAENEPQSAPKENKPYALNSE
KNFPMDMSDDYETQQWPERKLKHMQFPPMYEENSRDNPFKRTNEIVEEQYTPQSLATLESVFQELGKLTG
PNNQKRERMDEEQKLYTDDEDDIYKANNIAYEDVVGGEDWNPVEEKIESQTQEEVRDSKENIEKNEQIND
EMKRSGQLGIQEEGLRKESKDQLSDDVSKVIAYLKRLVNAAGSGRLQNGQNGERATRLFEKPLDSQSIYQ
LIEISRNLQIPPEDLIEMLKTGEKPNGSVEPERELDLPVDLDDISEADLDHPDLFQNRMLSKSGYPKTPG
GAGTEALPDGLSVEDILNLLGMESAANQKTSYFPNPYNQEKVLPRLPYGAGRSRSNQLPKAAWIPHVENR
QMAYENLNDKDQELGEYLARMLVKYPEIINSNQVKRVPGQGSSEGDLQEEEQIEQAIKEHLNQGSSQETD
KLAPVSKRFPVGPPKNDDTPNRQYWDEDLLMKVLEYLNQEKAEKGREHIAKRAMENM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003469
ORF Size 1851 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003469.5
RefSeq Size 2586 bp
RefSeq ORF 1854 bp
Locus ID 7857
UniProt ID P13521
Cytogenetics 2q36.1
Domains Granin
Protein Families Secreted Protein, Transmembrane
MW 70.7 kDa
Gene Summary The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq, Jul 2008]

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