Chromogranin C (SCG2) (NM_003469) Human Tagged ORF Clone

CAT#: RC205382

SCG2 (Myc-DDK-tagged)-Human secretogranin II (SCG2)

Product Images

Western blot validation of overexpression lysate (Cat# LY418654) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205382 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCG2 protein (Cat# TP305382). The protein was produced from HEK293T cells transfected with SCG2 cDNA clone (Cat# RC205382) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SCG2 (Cat# RC205382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCG2(Cat# TA811870). Positive lysates LY418654 (100ug) and LC418654 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chromogranin C
• Synonyms: CHGC; EM66; SgII; SN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205382 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGCAAAGACCCACTGGCTTGGAGCAGCCCTGTCTCTTATCCCTTTAATTTTCCTCATCTCTG
  GGGCTGAAGCAGCTTCATTTCAGAGAAACCAGCTGCTTCAGAAAGAACCAGACCTCAGGTTGGAAAATGT
  CCAAAAGTTTCCCAGTCCTGAAATGATCAGGGCTTTGGAGTACATAGAAAACCTCCGACAACAAGCTCAT
  AAGGAAGAAAGCAGCCCAGATTATAATCCCTACCAAGGTGTCTCTGTCCCCCTTCAGCAAAAAGAAAATG
  GCGATGAAAGCCACTTGCCCGAGAGGGATTCACTGAGTGAAGAAGACTGGATGAGAATAATACTCGAAGC
  TTTGAGACAGGCTGAAAATGAGCCTCAGTCTGCACCAAAAGAAAATAAGCCCTATGCCTTGAATTCAGAA
  AAGAACTTTCCAATGGACATGAGTGATGATTATGAGACACAGCAGTGGCCAGAAAGAAAGCTTAAGCACA
  TGCAATTCCCTCCTATGTATGAAGAGAATTCCAGGGATAACCCCTTTAAACGCACAAATGAAATAGTGGA
  GGAACAATATACTCCTCAAAGCCTTGCTACATTGGAATCTGTCTTCCAAGAGCTGGGGAAACTGACAGGA
  CCAAACAACCAGAAACGTGAGAGGATGGATGAGGAGCAAAAACTTTATACGGATGATGAAGATGATATCT
  ACAAGGCTAATAACATTGCCTATGAAGATGTGGTCGGGGGAGAAGACTGGAACCCAGTAGAGGAGAAAAT
  AGAGAGTCAAACCCAGGAAGAGGTGAGAGACAGCAAAGAGAATATAGAAAAAAATGAACAAATCAATGAT
  GAGATGAAACGCTCAGGGCAGCTTGGCATCCAGGAAGAAGGTCTTCGGAAAGAGAGTAAAGACCAACTCT
  CAGATGATGTCTCCAAAGTAATTGCCTATTTGAAAAGGTTAGTAAATGCTGCAGGAAGTGGGAGGTTACA
  GAATGGGCAAAATGGGGAAAGGGCCACCAGGCTTTTTGAGAAACCTCTTGATTCTCAGTCTATTTATCAG
  CTGATTGAAATCTCAAGGAATTTACAGATACCCCCAGAAGACTTAATTGAGATGCTCAAAACTGGGGAGA
  AGCCGAATGGATCAGTGGAACCGGAGCGGGAGCTTGACCTTCCTGTTGACCTAGATGACATCTCAGAGGC
  TGACTTAGACCATCCAGACCTGTTCCAAAATAGGATGCTCTCCAAGAGTGGCTACCCTAAAACACCTGGT
  GGTGCTGGGACTGAGGCCCTACCAGACGGGCTCAGTGTTGAGGATATTTTAAATCTTTTAGGGATGGAGA
  GTGCAGCAAATCAGAAAACGTCGTATTTTCCCAATCCATATAACCAGGAGAAAGTTCTGCCAAGGCTCCC
  TTATGGTGCTGGAAGATCTAGATCGAACCAGCTTCCCAAAGCTGCCTGGATTCCACATGTTGAAAACAGA
  CAGATGGCATATGAAAACCTGAACGACAAGGATCAAGAATTAGGTGAGTACTTGGCCAGGATGCTAGTTA
  AATACCCTGAGATCATTAATTCAAACCAAGTGAAGCGAGTTCCTGGTCAAGGCTCATCTGAAGGTGACCT
  GCAGGAAGAGGAACAAATTGAGCAGGCCATCAAAGAGCATTTGAATCAAGGCAGCTCTCAGGAGACTGAC
  AAGCTGGCCCCGGTGAGCAAAAGGTTCCCTGTGGGGCCCCCGAAGAATGATGATACCCCAAATAGGCAGT
  ACTGGGATGAAGATCTGTTAATGAAAGTGCTGGAATACCTCAACCAAGAAAAGGCAGAAAAGGGAAGGGA
  GCATATTGCTAAGAGAGCAATGGAAAATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205382 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAKTHWLGAALSLIPLIFLISGAEAASFQRNQLLQKEPDLRLENVQKFPSPEMIRALEYIENLRQQAH
  KEESSPDYNPYQGVSVPLQQKENGDESHLPERDSLSEEDWMRIILEALRQAENEPQSAPKENKPYALNSE
  KNFPMDMSDDYETQQWPERKLKHMQFPPMYEENSRDNPFKRTNEIVEEQYTPQSLATLESVFQELGKLTG
  PNNQKRERMDEEQKLYTDDEDDIYKANNIAYEDVVGGEDWNPVEEKIESQTQEEVRDSKENIEKNEQIND
  EMKRSGQLGIQEEGLRKESKDQLSDDVSKVIAYLKRLVNAAGSGRLQNGQNGERATRLFEKPLDSQSIYQ
  LIEISRNLQIPPEDLIEMLKTGEKPNGSVEPERELDLPVDLDDISEADLDHPDLFQNRMLSKSGYPKTPG
  GAGTEALPDGLSVEDILNLLGMESAANQKTSYFPNPYNQEKVLPRLPYGAGRSRSNQLPKAAWIPHVENR
  QMAYENLNDKDQELGEYLARMLVKYPEIINSNQVKRVPGQGSSEGDLQEEEQIEQAIKEHLNQGSSQETD
  KLAPVSKRFPVGPPKNDDTPNRQYWDEDLLMKVLEYLNQEKAEKGREHIAKRAMENM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003469
• ORF Size: 1851 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003469.5
• RefSeq Size: 2586 bp
• RefSeq ORF: 1854 bp
• Locus ID: 7857
• UniProt ID: P13521
• Cytogenetics: 2q36.1
• Domains: Granin
• Protein Families: Secreted Protein, Transmembrane
• MW: 70.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq, Jul 2008]