ICAM5 (NM_003259) Human Tagged ORF Clone

CAT#: RC205369

ICAM5 (Myc-DDK-tagged)-Human intercellular adhesion molecule 5, telencephalin (ICAM5)

Product Images

Circular map for RC205369

• 

  Western blot validation of overexpression lysate (Cat# LY418806) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205369 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ICAM5
• Synonyms: TLCN; TLN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205369 representing NM_003259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGGCCTTCGCCAGGGCTGCGCCGGGCGCTACTCGGCCTCTGGGCTGCTCTGGGCCTGGGGCTCT
  TCGGCCTCTCAGCGGTCTCGCAGGAGCCCTTCTGGGCGGACCTGCAGCCTCGCGTGGCGTTCGTGGAGCG
  CGGGGGCTCGCTGTGGCTGAATTGCAGCACCAACTGCCCTCGGCCGGAGCGCGGTGGCCTGGAGACCTCG
  CTGCGCCGAAACGGGACCCAGAGGGGTTTGCGTTGGTTGGCGCGGCAGCTGGTGGACATTCGCGAGCCGG
  AGACTCAGCCCGTCTGCTTCTTCCGCTGCGCGCGGCGCACACTACAGGCGCGTGGGCTCATTCGCACTTT
  CCAGCGACCAGATCGCGTAGAGCTGATGCCGCTGCCTCCCTGGCAGCCGGTGGGCGAGAACTTCACCCTG
  AGCTGTAGGGTCCCCGGCGCCGGGCCCCGTGCGAGCCTCACGCTGACCCTGCTGCGGGGCGCCCAGGAGC
  TGATCCGCCGCAGCTTCGCCGGTGAACCACCCCGAGCGCGGGGCGCGGTGCTCACAGCCACGGTACTGGC
  TCGGAGGGAGGACCATGGAGCCAATTTCTCGTGTCGCGCCGAGCTGGACCTGCGGCCGCACGGACTGGGA
  CTGTTTGAAAACAGCTCGGCCCCCAGAGAGCTCCGAACCTTCTCCCTGTCTCCGGATGCCCCGCGCCTCG
  CTGCTCCCCGGCTCTTGGAAGTTGGCTCGGAAAGGCCCGTGAGCTGCACTCTGGACGGACTGTTTCCAGC
  CTCAGAGGCCAGGGTCTACCTCGCACTGGGGGACCAGAATCTGAGTCCTGATGTCACCCTCGAAGGGGAC
  GCATTCGTGGCCACTGCCACAGCCACAGCTAGCGCAGAGCAGGAGGGTGCCAGGCAGCTGGTCTGCAACG
  TCACCCTGGGGGGCGAAAACCGGGAGACCCGGGAGAACGTGACCATCTACAGCTTCCCGGCACCACTCCT
  GACCCTGAGCGAACCCAGCGTCTCCGAGGGGCAGATGGTGACAGTAACCTGCGCAGCTGGGGCCCAAGCT
  CTGGTCACACTGGAGGGAGTTCCAGCCGCGGTCCCGGGGCAGCCCGCCCAGCTTCAGCTAAATGCCACCG
  AGAACGACGACAGACGCAGCTTCTTCTGCGACGCCACCCTCGATGTGGACGGGGAGACCCTGATCAAGAA
  CAGGAGCGCAGAGCTTCGTGTCCTATACGCTCCCCGGCTAGACGATTCGGACTGCCCCAGGAGTTGGACG
  TGGCCCGAGGGCCCAGAGCAGACGCTGCGCTGCGAGGCCCGCGGGAACCCAGAACCCTCAGTGCACTGTG
  CGCGCTCCGACGGCGGGGCCGTGCTGGCTCTGGGCCTGCTGGGTCCAGTCACTCGGGCGCTCTCAGGCAC
  TTACCGCTGCAAGGCGGCCAATGATCAAGGCGAGGCGGTCAAGGACGTAACGCTAACGGTGGAGTACGCA
  CCAGCGCTGGACAGCGTGGGCTGCCCAGAACGCATTACTTGGCTGGAGGGAACAGAAGCCTCGCTGAGCT
  GTGTGGCGCACGGGGTACCGCCGCCTGATGTGATCTGCGTGCGCTCTGGAGAACTCGGGGCCGTCATCGA
  GGGGCTGTTGCGTGTGGCCCGGGAGCATGCGGGCACTTACCGCTGCGAAGCCACCAACCCTCGGGGCTCT
  GCGGCCAAAAATGTGGCCGTCACGGTGGAATATGGCCCCAGGTTTGAGGAGCCGAGCTGCCCCAGCAATT
  GGACATGGGTGGAAGGATCTGGGCGCCTGTTTTCCTGTGAGGTCGATGGGAAGCCACAGCCAAGCGTGAA
  GTGCGTGGGCTCCGGGGGCGCCACTGAGGGGGTGCTGCTGCCGCTGGCACCCCCAGACCCTAGTCCCAGA
  GCTCCCAGAATCCCTAGAGTCCTGGCACCCGGTATCTACGTCTGCAACGCCACCAACCGCCACGGCTCCG
  TGGCCAAAACAGTCGTCGTGAGCGCGGAGTCGCCACCGGAGATGGATGAATCTACCTGCCCAAGTCACCA
  GACGTGGCTGGAAGGGGCTGAGGCTTCCGCGCTGGCCTGCGCCGCCCGGGGTCGCCCTTCCCCAGGAGTG
  CGCTGCTCTCGGGAAGGCATCCCATGGCCTGAGCAGCAGCGCGTGTCCCGAGAGGACGCGGGCACTTACC
  ACTGTGTGGCCACCAATGCGCATGGCACGGACTCCCGGACCGTCACTGTGGGCGTGGAATACCGGCCAGT
  GGTGGCCGAACTTGCTGCCTCGCCCCCTGGAGGCGTGCGCCCAGGAGGAAACTTCACGTTGACCTGCCGC
  GCGGAGGCCTGGCCTCCAGCCCAGATCAGCTGGCGCGCGCCCCCGGGGGCCCTCAACATCGGCCTGTCGA
  GCAACAACAGCACACTGAGCGTGGCAGGCGCCATGGGAAGCCACGGCGGCGAGTACGAGTGCGCAGCCAC
  CAACGCGCACGGGCGCCACGCGCGGCGCATCACGGTGCGCGTGGCCGGTCCGTGGCTATGGGTCGCCGTG
  GGCGGCGCGGCGGGGGGCGCGGCGCTGCTGGCCGCGGGGGCCGGCCTGGCCTTCTACGTGCAGTCCACCG
  CCTGCAAGAAGGGCGAGTACAACGTGCAGGAGGCCGAGAGCTCAGGCGAGGCCGTGTGTCTGAACGGAGC
  GGGCGGCGGCGCTGGCGGGGCGGCAGGCGCGGAGGGCGGACCCGAGGCGGCGGGGGGCGCGGCCGAGTCG
  CCGGCGGAGGGCGAGGTCTTCGCCATACAGCTGACATCGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205369 representing NM_003259
  Red=Cloning site Green=Tags(s)
  
  MPGPSPGLRRALLGLWAALGLGLFGLSAVSQEPFWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETS
  LRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTL
  SCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLG
  LFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGD
  AFVATATATASAEQEGARQLVCNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGAQA
  LVTLEGVPAAVPGQPAQLQLNATENDDRRSFFCDATLDVDGETLIKNRSAELRVLYAPRLDDSDCPRSWT
  WPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLGPVTRALSGTYRCKAANDQGEAVKDVTLTVEYA
  PALDSVGCPERITWLEGTEASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPRGS
  AAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKCVGSGGATEGVLLPLAPPDPSPR
  APRIPRVLAPGIYVCNATNRHGSVAKTVVVSAESPPEMDESTCPSHQTWLEGAEASALACAARGRPSPGV
  RCSREGIPWPEQQRVSREDAGTYHCVATNAHGTDSRTVTVGVEYRPVVAELAASPPGGVRPGGNFTLTCR
  AEAWPPAQISWRAPPGALNIGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGPWLWVAV
  GGAAGGAALLAAGAGLAFYVQSTACKKGEYNVQEAESSGEAVCLNGAGGGAGGAAGAEGGPEAAGGAAES
  PAEGEVFAIQLTSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003259
• ORF Size: 2772 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003259.4
• RefSeq Size: 3005 bp
• RefSeq ORF: 2775 bp
• Locus ID: 7087
• UniProt ID: Q9UMF0
• Cytogenetics: 19p13.2
• Protein Families: Transmembrane
• MW: 97.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases. [provided by RefSeq, Jul 2008]