GAPDHS (NM_014364) Human Tagged ORF Clone

CAT#: RC205362

GAPDHS (Myc-DDK-tagged)-Human glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS)

Product Images

Circular map for RC205362

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAPDHS (Cat# RC205362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAPDHS(Cat# TA505454). Positive lysates LY402320 (100ug) and LC402320 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402320) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205362 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GAPDHS protein (Cat# TP305362). The protein was produced from HEK293T cells transfected with GAPDHS cDNA clone (Cat# RC205362) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAPDHS
• Synonyms: GAPD2; GAPDH-2; GAPDS; HEL-S-278; HSD-35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205362 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAGCGCGACATCGTCCTCACCAATGTCACCGTTGTCCAGTTGCTGCGACAGCCGTGCCCGGTGA
  CCAGAGCACCGCCCCCACCTGAGCCTAAGGCTGAAGTAGAGCCCCAGCCACAACCAGAGCCCACACCAGT
  CAGGGAGGAAATAAAGCCACCACCGCCACCACTGCCTCCTCACCCCGCTACTCCTCCTCCTAAGATGGTG
  TCTGTGGCCCGGGAGCTGACTGTGGGCATCAATGGATTTGGACGCATCGGTCGCCTGGTCCTGCGCGCCT
  GCATGGAGAAGGGTGTTAAGGTGGTGGCTGTGAATGATCCATTCATTGACCCGGAATACATGGTGTACAT
  GTTTAAGTATGACTCCACCCACGGCCGATACAAGGGAAGTGTGGAATTCAGGAATGGACAACTGGTCGTG
  GACAACCATGAGATCTCTGTCTACCAGTGCAAAGAGCCCAAACAGATCCCCTGGAGGGCTGTCGGGAGCC
  CCTACGTGGTGGAGTCCACAGGCGTGTACCTCTCCATACAGGCAGCTTCGGACCACATCTCTGCAGGTGC
  TCAACGTGTGGTCATCTCCGCGCCCTCACCGGATGCACCAATGTTCGTCATGGGTGTCAATGAAAATGAC
  TATAACCCTGGCTCCATGAACATTGTGAGCAACGCGTCCTGCACCACCAACTGTTTGGCTCCCCTCGCCA
  AAGTCATCCACGAGCGATTTGGGATCGTGGAAGGGTTGATGACCACAGTCCATTCCTACACGGCCACCCA
  GAAGACAGTGGACGGGCCATCAAGGAAGGCCTGGCGAGATGGGCGGGGTGCCCACCAGAACATCATCCCA
  GCCTCCACTGGGGCTGCGAAAGCTGTGACCAAAGTCATCCCAGAGCTCAAAGGGAAGCTGACAGGGATGG
  CGTTCCGGGTACCAACCCCGGATGTGTCTGTCGTGGACCTGACCTGCCGCCTCGCCCAGCCTGCCCCCTA
  CTCAGCCATCAAGGAGGCTGTAAAAGCAGCAGCCAAGGGGCCCATGGCTGGCATCCTTGCCTACACCGAG
  GATGAGGTCGTCTCTACGGACTTCCTCGGTGATACCCACTCGTCCATCTTCGATGCTAAGGCCGGCATTG
  CGCTCAATGACAATTTCGTGAAGCTCATTTCATGGTACGACAACGAATATGGCTACAGTCACCGGGTGGT
  CGACCTCCTCCGCTACATGTTCAGCCGAGACAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205362 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMV
  SVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVV
  DNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNEND
  YNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIP
  ASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTE
  DEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014364
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014364.5
• RefSeq Size: 1492 bp
• RefSeq ORF: 1227 bp
• Locus ID: 26330
• UniProt ID: O14556
• Cytogenetics: 19q13.12
• Protein Families: Druggable Genome
• MW: 44.5 kDa
• Gene Summary: This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq, Jul 2008]