BAF53b (ACTL6B) (NM_016188) Human Tagged ORF Clone

CAT#: RC205358

ACTL6B (Myc-DDK-tagged)-Human actin-like 6B (ACTL6B)

Product Images

Circular map for RC205358

• 

  Western blot validation of overexpression lysate (Cat# LY402516) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205358 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BAF53b
• Synonyms: ACTL6; arpNalpha; BAF53B; DEE76; EIEE76; IDDSSAD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205358 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTGGTCTTTGACATTGGCTCCTTCTCAGTCC
  GCGCTGGGTACGCTGGGGAGGACTGTCCCAAGGCTGACTTCCCCACCACAGTGGGGCTGCTGGCCGCGGA
  GGAGGGGGGCGGGCTGGAGCTGGAGGGGGACAAAGAGAAGAAAGGGAAGATCTTCCACATCGACACCAAT
  GCCCTGCACGTGCCTCGGGATGGAGCGGAGGTCATGTCGCCCCTCAAGAATGGCATGATCGAGGACTGGG
  AGTGCTTCCGAGCCATCCTGGATCACACCTACAGCAAACACGTCAAGTCTGAGCCAAACCTGCACCCAGT
  GCTCATGTCCGAGGCTCCGTGGAACACACGGGCCAAGCGGGAGAAGCTGACAGAGCTGATGTTCGAGCAG
  TACAACATTCCTGCCTTCTTCTTATGCAAGACGGCTGTGCTCACCGCCTTTGCAAACGGGCGGTCCACTG
  GCCTCGTGCTGGACAGTGGAGCCACCCACACCACGGCCATTCCAGTACATGACGGCTACGTTCTGCAGCA
  AGGCATCGTCAAGTCCCCTCTGGCAGGGGACTTCATCTCCATGCAGTGCCGGGAGCTGTTCCAGGAGATG
  GCCATTGACATCATCCCACCTTACATGATCGCAGCCAAGGAGCCTGTCCGGGAGGGTGCCCCCCCAAACT
  GGAAGAAGAAGGAGAAGCTACCCCAGGTCTCCAAGTCCTGGCATAACTACATGTGTAATGAGGTGATCCA
  GGACTTCCAGGCCTCCGTGCTGCAGGTCTCAGACTCCCCCTACGATGAACAGGTGGCTGCACAAATGCCC
  ACAGTGCACTACGAGATGCCCAATGGCTACAATACAGACTACGGCGCCGAGCGACTCCGCATCCCTGAGG
  GCCTGTTTGATCCCTCGAACGTCAAGGGCCTGTCGGGGAACACCATGTTGGGTGTGGGCCACGTGGTGAC
  CACCAGCATCGGCATGTGTGACATTGATATTCGCCCGGGCCTGTACGGGAGTGTCATTGTCACCGGCGGG
  AACACACTGCTGCAGGGCTTCACTGACAGGCTCAATCGAGAGCTTTCCCAGAAGACCCCACCGAGCATGC
  GACTGAAACTCATTGCCAGCAACAGCACCATGGAGCGCAAGTTCAGCCCCTGGATCGGGGGTTCCATCCT
  GGCCTCACTGGGCACTTTCCAGCAGATGTGGATCTCCAAGCAGGAATATGAGGAGGGCGGGAAGCAGTGC
  GTGGAGCGAAAGTGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205358 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGGVYGGDEVGALVFDIGSFSVRAGYAGEDCPKADFPTTVGLLAAEEGGGLELEGDKEKKGKIFHIDTN
  ALHVPRDGAEVMSPLKNGMIEDWECFRAILDHTYSKHVKSEPNLHPVLMSEAPWNTRAKREKLTELMFEQ
  YNIPAFFLCKTAVLTAFANGRSTGLVLDSGATHTTAIPVHDGYVLQQGIVKSPLAGDFISMQCRELFQEM
  AIDIIPPYMIAAKEPVREGAPPNWKKKEKLPQVSKSWHNYMCNEVIQDFQASVLQVSDSPYDEQVAAQMP
  TVHYEMPNGYNTDYGAERLRIPEGLFDPSNVKGLSGNTMLGVGHVVTTSIGMCDIDIRPGLYGSVIVTGG
  NTLLQGFTDRLNRELSQKTPPSMRLKLIASNSTMERKFSPWIGGSILASLGTFQQMWISKQEYEEGGKQC
  VERKCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016188
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016188.5
• RefSeq Size: 1550 bp
• RefSeq ORF: 1281 bp
• Locus ID: 51412
• UniProt ID: O94805
• Cytogenetics: 7q22.1
• Domains: ACTIN
• Protein Families: Druggable Genome
• MW: 46.9 kDa
• Gene Summary: The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]