TIF1 alpha (TRIM24) (NM_015905) Human Tagged ORF Clone

CAT#: RC205332

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TRIM24 (Myc-DDK-tagged)-Human tripartite motif containing 24 (TRIM24), transcript variant 1



  "NM_015905" in other vectors (6)

Reconstitution Protocol

USD 1,371.00

USD 2,056.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Other products for "TRIM24"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM24
Synonyms hTIF1; PTC6; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205332 representing NM_015905
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCGGTGGAGAAGGCGGTGGCGGCGGCGGCAGCGGCCTCGGCTGCGGCCTCCGGGGGGCCCT
CGGCGGCGCCGAGCGGGGAGAACGAGGCCGAGAGTCGGCAGGGCCCGGACTCGGAGCGCGGCGGCGAGGC
GGCCCGGCTCAACCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGCGCCCAAGCTG
CTGCCCTGCCTGCACTCTTTCTGCCAGCGCTGCCTGCCCGCGCCCCAGCGCTACCTCATGCTGCCCGCGC
CCATGCTGGGCTCGGCCGAGACCCCGCCACCCGTCCCTGCCCCCGGCTCGCCGGTCAGCGGCTCGTCGCC
GTTCGCCACCCAAGTTGGAGTCATTCGTTGCCCAGTTTGCAGCCAAGAATGTGCAGAGAGACACATCATA
GATAACTTTTTTGTGAAGGACACTACTGAGGTTCCCAGCAGTACAGTAGAAAAGTCAAATCAGGTATGTA
CAAGCTGTGAGGACAACGCAGAAGCCAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACGTG
TATCAGAGCTCATCAGAGGGTAAAGTTCACAAAAGACCACACTGTCAGACAGAAAGAGGAAGTATCTCCA
GAGGCAGTTGGTGTCACCAGCCAGCGACCAGTGTTTTGTCCTTTTCATAAAAAGGAGCAGCTGAAGCTGT
ACTGTGAGACATGTGACAAACTGACATGTCGAGACTGTCAGTTGTTAGAACATAAAGAGCATAGATACCA
ATTTATAGAAGAAGCTTTTCAGAATCAGAAAGTGATCATAGATACACTAATCACCAAACTGATGGAAAAA
ACAAAATACATAAAATTCACAGGAAATCAGATCCAAAACAGAATTATTGAAGTAAATCAAAATCAAAAGC
AGGTGGAACAGGATATTAAAGTTGCTATATTTACACTGATGGTAGAAATAAATAAAAAAGGAAAAGCTCT
ACTGCATCAGTTAGAGAGCCTTGCAAAGGACCATCGCATGAAACTTATGCAACAACAACAGGAAGTGGCT
GGACTCTCTAAACAATTGGAGCATGTCATGCATTTTTCTAAATGGGCAGTTTCCAGTGGCAGCAGTACAG
CATTACTTTATAGCAAACGACTGATTACATACCGGTTACGGCACCTCCTTCGTGCAAGGTGTGATGCATC
CCCAGTGACCAACAACACCATCCAATTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATCATCAACTTA
GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAACAGA
ATTCACAGCCACCAAGTGGTTTATCATCAAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA
ATTACGGCTCCAGCATATGCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT
GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAACCTTCCATCTCTCATCAGCAACCGCCTC
CACGTTTGATAAACTTTCAGAATCACAGCCCCAAACCCAATGGACCAGTTCTTCCTCCTCATCCTCAACA
ACTGAGATATCCACCAAACCAGAACATACCACGACAAGCAATAAAGCCAAACCCCCTACAGATGGCTTTC
TTGGCTCAACAAGCCATAAAACAGTGGCAGATCAGCAGTGGACAGGGAACCCCATCAACTACCAACAGCA
CATCCTCTACTCCTTCCAGCCCCACGATTACTAGTGCAGCAGGATATGATGGAAAGGCTTTTGGTTCACC
TATGATCGATTTGAGCTCACCAGTGGGAGGGTCTTATAATCTTCCCTCTCTTCCGGATATTGACTGTTCA
AGTACTATTATGCTGGACAATATTGTGAGGAAAGATACTAATATAGATCATGGCCAGCCAAGACCACCCT
CAAACAGAACGGTCCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGACCTGTTACTATGAC
TAGTGTACACCCCCCAATACGTTCACCTAGTGCCTCCAGCGTTGGAAGCCGAGGAAGCTCTGGCTCTTCC
AGCAAACCAGCAGGAGCTGACTCTACACACAAAGTCCCAGTGGTCATGCTGGAGCCAATTCGAATAAAAC
AAGAAAACAGTGGACCACCGGAAAATTATGATTTCCCTGTTGTTATAGTGAAGCAAGAATCAGATGAAGA
ATCTAGGCCTCAAAATGCCAATTATCCAAGAAGCATACTCACCTCCCTGCTCTTAAATAGCAGTCAGAGC
TCTACTTCTGAGGAGACTGTGCTAAGATCAGATGCCCCTGATAGTACAGGAGATCAACCTGGACTTCACC
AGGACAATTCCTCAAATGGAAAGTCTGAATGGTTGGATCCTTCCCAGAAGTCACCTCTTCATGTTGGAGA
GACAAGGAAAGAGGATGACCCCAATGAGGACTGGTGTGCAGTTTGTCAAAACGGAGGGGAACTCCTCTGC
TGTGAAAAGTGCCCCAAAGTATTCCATCTTTCTTGTCATGTGCCCACATTGACAAATTTTCCAAGTGGAG
AGTGGATTTGCACTTTCTGCCGAGACTTATCTAAACCAGAAGTTGAATATGATTGTGATGCTCCCAGTCA
CAACTCAGAAAAAAAGAAAACTGAAGGCCTTGTTAAGTTAACACCTATAGATAAAAGGAAGTGTGAGCGC
CTACTTTTATTTCTTTACTGCCATGAAATGAGCCTGGCTTTTCAAGACCCTGTTCCTCTAACTGTGCCTG
ATTATTACAAAATAATTAAAAATCCAATGGATTTGTCAACCATCAAGAAAAGACTACAAGAAGATTATTC
CATGTACTCAAAACCTGAAGATTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTCAAT
GAGCCTGATTCAGAAGTAGCCAATGCTGGTATAAAACTTGAAAATTATTTTGAAGAACTTCTAAAGAACC
TCTATCCAGAAAAAAGGTTTCCCAAACCAGAATTCAGGAATGAATCAGAAGATAATAAATTTAGTGATGA
TTCAGATGATGACTTTGTACAGCCCCGGAAGAAACGCCTCAAAAGCATTGAAGAACGCCAGTTGCTTAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205332 representing NM_015905
Red=Cloning site Green=Tags(s)

MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKL
LPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHII
DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP
EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK
TKYIKFTGNQIQNRIIEVNQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA
GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNNTIQFHCDPSFWAQNIINL
GSLVIEDKESQPQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAP
VGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAF
LAQQAIKQWQISSGQGTPSTTNSTSSTPSSPTITSAAGYDGKAFGSPMIDLSSPVGGSYNLPSLPDIDCS
STIMLDNIVRKDTNIDHGQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGSS
SKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNANYPRSILTSLLLNSSQS
STSEETVLRSDAPDSTGDQPGLHQDNSSNGKSEWLDPSQKSPLHVGETRKEDDPNEDWCAVCQNGGELLC
CEKCPKVFHLSCHVPTLTNFPSGEWICTFCRDLSKPEVEYDCDAPSHNSEKKKTEGLVKLTPIDKRKCER
LLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYSMYSKPEDFVADFRLIFQNCAEFN
EPDSEVANAGIKLENYFEELLKNLYPEKRFPKPEFRNESEDNKFSDDSDDDFVQPRKKRLKSIEERQLLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015905
ORF Size 3150 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015905.3
RefSeq Size 4007 bp
RefSeq ORF 3153 bp
Locus ID 8805
UniProt ID O15164
Cytogenetics 7q33-q34
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
MW 116.8 kDa
Gene Summary The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

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