Monoamine Oxidase B (MAOB) (NM_000898) Human Tagged ORF Clone

CAT#: RC205320

  • TrueORF®

MAOB (Myc-DDK-tagged)-Human monoamine oxidase B (MAOB), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro


  "NM_000898" in other vectors (6)

Reconstitution Protocol

USD 726.00

5 Days*

Size
    • 10 ug

Product Images

Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol Monoamine Oxidase B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205320 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAACAAATGCGACGTGGTCGTGGTGGGGGGCGGCATCTCAGGTATGGCAGCAGCCAAACTTCTGC
ATGACTCTGGACTGAATGTGGTTGTTCTGGAAGCCCGGGACCATGTGGGAGGCAGGACTTACACTCTTAG
GAACCAAAAGGTTAAATATGTGGACCTTGGAGGATCCTATGTTGGACCAACCCAGAATCGTATCTTGAGA
TTAGCCAAGGAGCTAGGATTGGAGACCTACAAAGTGAATGAGGTTGAGCGTCTGATCCACCATGTAAAGG
GCAAATCATACCCCTTCAGGGGGCCATTCCCACCTGTATGGAATCCAATTACCTACTTAGATCATAACAA
CTTTTGGAGGACAATGGATGACATGGGGCGAGAGATTCAGAGTGATGCCCCATGGAAGGCTCCCCTTGCA
GAAGAGTGGGACAACATGACAATGAAGGAGCTACTGGACAAGCTCTGCTGGACTGAATCTGCAAAGCAGC
TTGCCACTCTCTTTGTGAACCTGTGTGTCACTGCAGAGACCCATGAGGTCTCTGCTCTCTGGTTCCTGTG
GTATGTGAAGCAGTGTGGAGGCACAACAAGAATCATCTCGACAACAAATGGAGGACAGGAGAGGAAATTT
GTGGGCGGATCTGGTCAAGTGAGTGAGCGGATAATGGACCTCCTTGGAGACCGAGTGAAGCTGGAGAGGC
CTGTGATCTACATTGACCAGACAAGAGAAAATGTCCTTGTGGAGACCCTAAACCATGAGATGTATGAGGC
TAAATATGTGATTAGTGCTATTCCTCCTACTCTGGGCATGAAGATTCACTTCAATCCCCCTCTGCCAATG
ATGAGAAACCAGATGATCACTCGTGTGCCTTTGGGTTCAGTCATCAAGTGTATAGTTTATTATAAAGAGC
CTTTCTGGAGGAAAAAGGATTACTGTGGAACCATGATTATTGATGGAGAAGAAGCTCCAGTTGCCTACAC
GTTGGATGATACCAAACCTGAAGGCAACTATGCTGCCATAATGGGATTTATCCTGGCCCACAAAGCCAGA
AAACTGGCACGTCTTACCAAAGAGGAAAGGTTGAAGAAACTTTGTGAACTCTATGCCAAGGTTCTGGGTT
CCCTAGAAGCTCTGGAGCCAGTGCATTATGAAGAAAAGAACTGGTGTGAGGAGCAGTACTCTGGGGGCTG
CTACACAACTTATTTCCCCCCTGGGATCCTGACTCAATATGGAAGGGTTCTACGCCAGCCAGTGGACAGG
ATTTACTTTGCAGGCACCGAGACTGCCACACACTGGAGCGGCTACATGGAGGGGGCTGTAGAGGCCGGGG
AGAGAGCAGCCCGAGAGATCCTGCATGCCATGGGGAAGATTCCAGAGGATGAAATCTGGCAGTCAGAACC
AGAGTCTGTGGATGTCCCTGCACAGCCCATCACCACCACCTTTTTGGAGAGACATTTGCCCTCCGTGCCA
GGCCTGCTCAGGCTGATTGGATTGACCACCATCTTTTCAGCAACGGCTCTTGGCTTCCTGGCCCACAAAA
GGGGGCTACTTGTGAGAGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205320 protein sequence
Red=Cloning site Green=Tags(s)

MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDHVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILR
LAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIQSDAPWKAPLA
EEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKF
VGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPM
MRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDTKPEGNYAAIMGFILAHKAR
KLARLTKEERLKKLCELYAKVLGSLEALEPVHYEEKNWCEEQYSGGCYTTYFPPGILTQYGRVLRQPVDR
IYFAGTETATHWSGYMEGAVEAGERAAREILHAMGKIPEDEIWQSEPESVDVPAQPITTTFLERHLPSVP
GLLRLIGLTTIFSATALGFLAHKRGLLVRV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000898
ORF Size 1560 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000898.2
RefSeq Size 2611 bp
RefSeq ORF 1563 bp
Locus ID 4129
UniProt ID P27338
Cytogenetics Xp11.3
Domains Amino_oxidase
Protein Families Druggable Genome, Transmembrane
Protein Pathways Arginine and proline metabolism, Drug metabolism - cytochrome P450, Glycine, serine and threonine metabolism, Histidine metabolism, Metabolic pathways, Phenylalanine metabolism, Tryptophan metabolism, Tyrosine metabolism
MW 58.8 kDa
Gene Summary The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.