RIOK3 (NM_145906) Human Tagged ORF Clone

CAT#: RC205314

RIOK3 (Myc-DDK-tagged)-Human RIO kinase 3 (yeast) (RIOK3), transcript variant 2

Product Images

Circular map for RC205314

• 

  Western blot validation of overexpression lysate (Cat# LY407842) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205314 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIOK3
• Synonyms: SUDD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205314 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGCAAGTGTCCAT
  GGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGTGAACAGCTGGCCAAAGAATT
  GCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCTGTTGCTGAAGGACCATTTATTACTGGAGAA
  AACATTGATACTTCCAGTGACCTTATGCTGGCTCAGATGCTACAGATGGAATATGACAGAGAATATGATG
  CACAGCTTAGGCGTGAAAAAAAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCG
  AAAAGTGCATCCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
  CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAAGATATCACCA
  CCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAAAATTTTGCACCTGAGTTTCA
  GGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAACCATGTTTTCAATGCTTTAAAACAACATGCC
  TACTCAGAAGAACGTCGAAGTGCCCGCCTACATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTG
  ATCCTAAGACACGTTTACTTATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTAT
  TAGTACAGGAAAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
  AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAGAATCGTGACA
  AATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAATCCACGTAAGATCATCCGCAT
  GTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAGAGAGCTGGAATTCCTTGTCCAACAGTTGTA
  CTACTGAAGAAACACATTTTAGTTATGTCTTTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAG
  AAGTAAAGCTCAATAGTGAAGAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATA
  TCATGAATGTACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTGAGG
  AGCACATTTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205314 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGE
  NIDTSSDLMLAQMLQMEYDREYDAQLRREKKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDD
  PYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHA
  YSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDS
  KVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVV
  LLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVR
  STFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145906
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145906.1, NP_665913.1
• RefSeq Size: 3589 bp
• RefSeq ORF: 1274 bp
• Locus ID: 8780
• Cytogenetics: 18q11.2
• Domains: RIO
• Protein Families: Druggable Genome, Protein Kinase
• MW: 48.4 kDa
• Gene Summary: This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017]