NEUROD2 (NM_006160) Human Tagged ORF Clone

CAT#: RC205293

NEUROD2 (Myc-DDK-tagged)-Human neurogenic differentiation 2 (NEUROD2)

Product Images

Circular map for RC205293

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  Western blot validation of overexpression lysate (Cat# LY416831) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205293 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NEUROD2 protein (Cat# TP305293). The protein was produced from HEK293T cells transfected with NEUROD2 cDNA clone (Cat# RC205293) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEUROD2
• Synonyms: bHLHa1; DEE72; EIEE72; NDRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205293 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCCGCCTGTTCAGCGAGCCCGGCCTTCTCTCGGACGTGCCCAAGTTCGCCAGCTGGGGCGACG
  GCGAAGACGACGAGCCGAGGAGCGACAAGGGCGACGCGCCGCCACCGCCACCGCCTGCGCCCGGGCCAGG
  GGCTCCGGGGCCAGCCCGGGCGGCCAAGCCAGTCCCTCTCCGTGGAGAAGAGGGGACGGAGGCCACGTTG
  GCCGAGGTCAAGGAGGAAGGCGAGCTGGGGGGAGAGGAGGAGGAGGAAGAGGAGGAGGAAGAAGGACTGG
  ACGAGGCGGAGGGCGAGCGGCCCAAGAAGGGCGGGCCCAAGAAGCGCAAGATGACCAAGGCGCGCTTGGA
  GCGCTCCAAGCTTCGGCGGCAGAAGGCGAACGCGCGGGAGCGCAACCGCATGCACGACCTGAACGCAGCC
  CTGGACAACCTGCGCAAGGTGGTGCCCTGCTACTCCAAGACGCAGAAGCTGTCCAAGATCGAGACGCTGC
  GCCTAGCCAAGAACTATATCTGGGCGCTCTCGGAGATCCTGCGCTCCGGCAAGCGGCCAGACCTAGTGTC
  CTACGTGCAGACTCTGTGCAAGGGTCTGTCGCAGCCCACCACCAATCTGGTGGCCGGCTGTCTGCAGCTC
  AACTCTCGCAACTTCCTCACGGAGCAAGGCGCCGACGGTGCCGGCCGCTTCCACGGCTCGGGCGGCCCGT
  TCGCCATGCACCCCTACCCGTACCCGTGCTCGCGCCTGGCGGGCGCACAGTGCCAGGCGGCCGGCGGCCT
  GGGCGGCGGCGCGGCGCACGCCCTGCGGACCCACGGCTACTGCGCAGCCTACGAGACGCTGTATGCGGCG
  GCAGGCGGTGGCGGCGCGAGCCCGGACTACAACAGCTCCGAGTACGAGGGCCCGCTCAGCCCCCCGCTCT
  GTCTCAATGGCAACTTCTCACTCAAGCAGGACTCCTCGCCCGACCACGAGAAAAGCTACCACTACTCTAT
  GCACTACTCGGCGCTGCCCGGTTCGCGGCCCACGGGCCACGGGCTAGTCTTCGGCTCGTCGGCTGTGCGC
  GGGGGCGTCCACTCGGAGAATCTCTTGTCTTACGATATGCACCTTCACCACGACCGGGGCCCCATGTACG
  AGGAGCTCAATGCGTTTTTTCATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205293 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLTRLFSEPGLLSDVPKFASWGDGEDDEPRSDKGDAPPPPPPAPGPGAPGPARAAKPVPLRGEEGTEATL
  AEVKEEGELGGEEEEEEEEEEGLDEAEGERPKKGGPKKRKMTKARLERSKLRRQKANARERNRMHDLNAA
  LDNLRKVVPCYSKTQKLSKIETLRLAKNYIWALSEILRSGKRPDLVSYVQTLCKGLSQPTTNLVAGCLQL
  NSRNFLTEQGADGAGRFHGSGGPFAMHPYPYPCSRLAGAQCQAAGGLGGGAAHALRTHGYCAAYETLYAA
  AGGGGASPDYNSSEYEGPLSPPLCLNGNFSLKQDSSPDHEKSYHYSMHYSALPGSRPTGHGLVFGSSAVR
  GGVHSENLLSYDMHLHHDRGPMYEELNAFFHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006160
• ORF Size: 1146 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006160.2, NP_006151.2
• RefSeq Size: 3048 bp
• RefSeq ORF: 1149 bp
• Locus ID: 4761
• UniProt ID: Q15784
• Cytogenetics: 17q12
• Domains: HLH
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• MW: 41.3 kDa
• Gene Summary: This gene encodes a member of the neuroD family of neurogenic basic helix-loop-helix (bHLH) proteins. Expression of this gene can induce transcription from neuron-specific promoters, such as the GAP-43 promoter, which contain a specific DNA sequence known as an E-box. The product of the human gene can induce neurogenic differentiation in non-neuronal cells in Xenopus embryos, and is thought to play a role in the determination and maintenance of neuronal cell fates. [provided by RefSeq, Jul 2008]