EDIL3 (NM_005711) Human Tagged ORF Clone

CAT#: RC205282

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EDIL3 (Myc-DDK-tagged)-Human EGF-like repeats and discoidin I-like domains 3 (EDIL3)



  "NM_005711" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol EDIL3
Synonyms DEL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205282 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGCTCGGTAGCCGTCTGGCTCTTGGTCGGGCTCAGCCTCAGTGTCCCCCAGTTCGGCAAAGGTG
ATATTTGTGATCCCAATCCATGTGAAAATGGAGGTATCTGTTTGCCAGGATTGGCTGATGGTTCCTTTTC
CTGTGAGTGTCCAGATGGCTTCACAGACCCCAACTGTTCTAGTGTTGTGGAGGTTGCATCAGATGAAGAA
GAACCAACTTCAGCAGGTCCCTGCACTCCTAATCCATGCCATAATGGAGGAACCTGTGAAATAAGTGAAG
CATACCGAGGGGATACATTCATAGGCTATGTTTGTAAATGTCCCCGAGGATTTAATGGGATTCACTGTCA
GCACAACATAAATGAATGCGAAGTTGAGCCTTGCAAAAATGGTGGAATATGTACAGATCTTGTTGCTAAC
TATTCCTGTGAGTGCCCAGGCGAATTTATGGGAAGAAATTGTCAATACAAATGCTCAGGCCCACTGGGAA
TTGAAGGTGGAATTATATCAAACCAGCAAATCACAGCTTCCTCTACTCACCGAGCTCTTTTTGGACTCCA
AAAATGGTATCCCTACTATGCACGTCTTAATAAGAAGGGGCTTATAAATGCGTGGACAGCTGCAGAAAAT
GACAGATGGCCGTGGATTCAGATAAATTTGCAAAGGAAAATGAGAGTTACTGGTGTGATTACCCAAGGAG
CCAAGAGGATTGGAAGCCCAGAGTATATAAAATCCTACAAAATTGCCTACAGTAATGATGGAAAGACTTG
GGCAATGTACAAAGTGAAAGGCACCAATGAAGACATGGTGTTTCGTGGAAACATTGATAACAACACTCCA
TATGCTAACTCTTTCACACCCCCCATAAAAGCTCAGTATGTAAGACTCTATCCCCAAGTTTGTCGAAGAC
ATTGCACTTTGCGAATGGAACTTCTTGGCTGTGAACTGTCGGGTTGTTCTGAGCCTCTGGGTATGAAATC
AGGACATATACAAGACTATCAGATCACTGCCTCCAGCATCTTCAGAACGCTCAACATGGACATGTTCACT
TGGGAACCAAGGAAAGCTCGGCTGGACAAGCAAGGCAAAGTGAATGCCTGGACCTCTGGCCACAATGACC
AGTCACAATGGTTACAGGTGGATCTTCTTGTTCCAACCAAAGTGACTGGCATCATTACACAAGGAGCTAA
AGATTTTGGTCATGTACAGTTTGTTGGCTCCTACAAACTGGCTTACAGCAATGATGGAGAACACTGGACT
GTATACCAGGATGAAAAGCAAAGAAAAGATAAGGTTTTCCAGGGAAATTTTGACAATGACACTCACAGAA
AAAATGTCATCGACCCTCCCATCTATGCACGACACATAAGAATCCTTCCTTGGTCCTGGTACGGGAGGAT
CACATTGCGGTCAGAGCTGCTGGGCTGCACAGAGGAGGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205282 protein sequence
Red=Cloning site Green=Tags(s)

MKRSVAVWLLVGLSLSVPQFGKGDICDPNPCENGGICLPGLADGSFSCECPDGFTDPNCSSVVEVASDEE
EPTSAGPCTPNPCHNGGTCEISEAYRGDTFIGYVCKCPRGFNGIHCQHNINECEVEPCKNGGICTDLVAN
YSCECPGEFMGRNCQYKCSGPLGIEGGIISNQQITASSTHRALFGLQKWYPYYARLNKKGLINAWTAAEN
DRWPWIQINLQRKMRVTGVITQGAKRIGSPEYIKSYKIAYSNDGKTWAMYKVKGTNEDMVFRGNIDNNTP
YANSFTPPIKAQYVRLYPQVCRRHCTLRMELLGCELSGCSEPLGMKSGHIQDYQITASSIFRTLNMDMFT
WEPRKARLDKQGKVNAWTSGHNDQSQWLQVDLLVPTKVTGIITQGAKDFGHVQFVGSYKLAYSNDGEHWT
VYQDEKQRKDKVFQGNFDNDTHRKNVIDPPIYARHIRILPWSWYGRITLRSELLGCTEEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005711
ORF Size 1440 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005711.2
RefSeq Size 4771 bp
RefSeq ORF 1443 bp
Locus ID 10085
UniProt ID O43854
Cytogenetics 5q14.3
Domains F5_F8_type_C, EGF_CA, EGF, EGF
Protein Families Druggable Genome, Secreted Protein
MW 53.8 kDa
Gene Summary The protein encoded by this gene is an integrin ligand. It plays an important role in mediating angiogenesis and may be important in vessel wall remodeling and development. It also influences endothelial cell behavior. [provided by RefSeq, Jul 2008]

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