GNPTG (NM_032520) Human Tagged ORF Clone

CAT#: RC205280

GNPTG (Myc-DDK-tagged)-Human N-acetylglucosamine-1-phosphate transferase, gamma subunit (GNPTG)

Product Images

Circular map for RC205280

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  Western blot validation of overexpression lysate (Cat# LY403165) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205280 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GNPTG protein (Cat# TP305280). The protein was produced from HEK293T cells transfected with GNPTG cDNA clone (Cat# RC205280) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNPTG
• Synonyms: C16orf27; GNPTAG; LP2537; RJD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205280 representing NM_032520
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGGCTGGCGCGGCTCCTGTTGCTCCTCGGGCTCTCGGCCGGCGGGCCCGCGCCGGCAGGTG
  CAGCGAAGATGAAGGTGGTGGAGGAGCCCAACGCGTTTGGGGTGAACAACCCGTTCTTGCCTCAGGCCAG
  TCGCCTCCAGGCCAAGAGGGATCCTTCACCCGTGTCTGGACCCGTGCATCTCTTCCGACTCTCGGGCAAG
  TGCTTCAGCCTGGTGGAGTCCACGTACAAGTATGAGTTCTGCCCGTTCCACAACGTGACCCAGCACGAGC
  AGACCTTCCGCTGGAACGCCTACAGTGGGATCCTCGGCATCTGGCACGAGTGGGAGATCGCCAACAACAC
  CTTCACGGGCATGTGGATGAGGGACGGTGACGCCTGCCGTTCCCGGAGCCGGCAGAGCAAGGTGGAGCTG
  GCGTGTGGAAAAAGCAACCGGCTGGCCCATGTGTCCGAGCCGAGCACCTGCGTCTACGCGCTGACGTTCG
  AGACCCCCCTCGTCTGCCACCCCCACGCCTTGCTAGTGTACCCAACCCTGCCAGAGGCCCTGCAGCGGCA
  GTGGGACCAGGTAGAGCAGGACCTGGCCGATGAGCTGATCACCCCCCAGGGCCATGAGAAGTTGCTGAGG
  ACACTTTTTGAGGATGCTGGCTACTTAAAGACCCCAGAAAATGAACCCACCCAGCTGGAGGGAGGTCCTG
  ACAGCTTGGGGTTTGAGACCCTGGAAAACTGCAGGAAGGCTCATAAAGAACTCTCAAAGGAGATCAAAAG
  GCTGAAAGGTTTGCTCACCCAGCACGGCATCCCCTACACGAGGCCCACAGAAACTTCCAACTTGGAGCAC
  TTGGGCCACGAGACGCCCAGAGCCAAGTCTCCAGAGCAGCTGCGGGGTGACCCAGGACTGCGTGGGAGTT
  TG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205280 representing NM_032520
  Red=Cloning site Green=Tags(s)
  
  MAAGLARLLLLLGLSAGGPAPAGAAKMKVVEEPNAFGVNNPFLPQASRLQAKRDPSPVSGPVHLFRLSGK
  CFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIANNTFTGMWMRDGDACRSRSRQSKVEL
  ACGKSNRLAHVSEPSTCVYALTFETPLVCHPHALLVYPTLPEALQRQWDQVEQDLADELITPQGHEKLLR
  TLFEDAGYLKTPENEPTQLEGGPDSLGFETLENCRKAHKELSKEIKRLKGLLTQHGIPYTRPTETSNLEH
  LGHETPRAKSPEQLRGDPGLRGSL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032520
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032520.5
• RefSeq Size: 1228 bp
• RefSeq ORF: 918 bp
• Locus ID: 84572
• UniProt ID: Q9UJJ9
• Cytogenetics: 16p13.3
• Protein Families: Secreted Protein
• MW: 33.8 kDa
• Gene Summary: This gene encodes the gamma sunbunit of the N-acetylglucosamine-1-phosphotransferase complex. This hexameric complex, composed of alpha, beta and gamma subunits, catalyzes the first step in synthesis of a mannose 6-phosphate lysosomal recognition marker. This enzyme complex is necessary for targeting of lysosomal hydrolases to the lysosome. Mutations in the gene encoding the gamma subunit have been associated with mucolipidosis IIIC, also known as mucolipidosis III gamma.[provided by RefSeq, Feb 2010]