ALF (GTF2A1L) (NM_006872) Human Tagged ORF Clone

CAT#: RC205264

GTF2A1L (Myc-DDK-tagged)-Human general transcription factor IIA, 1-like (GTF2A1L), transcript variant 1

Product Images

Circular map for RC205264

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GTF2A1L (Cat# RC205264, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2A1L (Cat# TA810959)(1:2000). Positive lysates LY416352 (100ug) and LC416352 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416352) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALF
• Synonyms: ALF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205264 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGCCTCAACCCGGTGCCTAAACTCTACAGATCTGTAATTGAAGATGTAATTGAAGGAGTTCGGA
  ATCTATTTGCTGAAGAAGGTATAGAGGAACAAGTTTTAAAAGACTTGAAGCAGCTCTGGGAAACCAAGGT
  TTTGCAGTCTAAAGCAACAGAAGACTTCTTCAGAAATAGCATCCAATCACCTCTGTTTACTCTTCAGTTG
  CCGCACAGCTTGCACCAAACATTGCAATCGTCAACAGCATCATTAGTTATTCCTGCTGGTAGAACTCTTC
  CAAGTTTTACCACAGCAGAACTGGGCACTTCAAACTCCAGTGCAAACTTTACTTTTCCTGGTTATCCCAT
  TCATGTACCAGCAGGTGTGACACTACAGACTGTATCTGGTCACCTTTATAAAGTCAATGTACCAATTATG
  GTGACAGAGACTTCTGGAAGAGCAGGTATTCTTCAGCATCCAATTCAGCAAGTATTTCAACAGCTTGGCC
  AGCCTTCAGTAATACAAACTAGTGTTCCACAATTGAATCCATGGTCTCTTCAAGCAACTACTGAAAAATC
  ACAGAGAATTGAAACCGTGCTACAGCAACCCGCAATTCTACCTTCTGGGCCAGTAGATAGGAAACACTTA
  GAAAATGCCACCAGTGATATACTTGTATCTCCTGGAAATGAGCATAAAATCGTGCCTGAAGCTTTGTTGT
  GTCATCAGGAAAGTTCTCACTATATCAGTCTTCCAGGTGTTGTATTTTCTCCACAGGTCTCTCAAACAAA
  TTCTAATGTGGAGTCAGTGCTCAGTGGTTCAGCTAGCATGGCTCAAAATCTGCATGATGAGTCCCTCTCC
  ACAAGCCCTCATGGGGCTCTCCACCAGCACGTGACTGATATTCAGCTTCATATTCTTAAAAATAGGATGT
  ATGGATGTGATTCTGTAAAGCAACCAAGAAATATAGAGGAACCCAGCAACATACCTGTATCAGAGAAGGA
  TTCTAATTCTCAGGTGGATTTAAGCATTCGGGTTACTGATGATGATATTGGTGAAATAATTCAAGTAGAT
  GGAAGCGGTGATACATCTTCCAATGAAGAAATAGGAAGTACAAGAGATGCAGATGAGAATGAATTTCTAG
  GGAATATTGACGGGGGAGATCTGAAGGTACCTGAAGAAGAAGCTGACAGTATTTCAAATGAGGATTCAGC
  CACAAACAGTAGTGATAATGAAGACCCTCAAGTAAACATTGTAGAAGAGGACCCTTTAAATTCTGGAGAT
  GATGTTAGTGAACAGGATGTGCCAGACCTGTTTGACACGGATAATGTTATTGTCTGTCAGTATGATAAGA
  TTCATCGAAGCAAGAACAAATGGAAATTCTATTTGAAAGATGGTGTTATGTGTTTTGGAGGGAGAGACTA
  TGTATTTGCAAAAGCCATTGGTGATGCAGAGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205264 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MACLNPVPKLYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWETKVLQSKATEDFFRNSIQSPLFTLQL
  PHSLHQTLQSSTASLVIPAGRTLPSFTTAELGTSNSSANFTFPGYPIHVPAGVTLQTVSGHLYKVNVPIM
  VTETSGRAGILQHPIQQVFQQLGQPSVIQTSVPQLNPWSLQATTEKSQRIETVLQQPAILPSGPVDRKHL
  ENATSDILVSPGNEHKIVPEALLCHQESSHYISLPGVVFSPQVSQTNSNVESVLSGSASMAQNLHDESLS
  TSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEIIQVD
  GSGDTSSNEEIGSTRDADENEFLGNIDGGDLKVPEEEADSISNEDSATNSSDNEDPQVNIVEEDPLNSGD
  DVSEQDVPDLFDTDNVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIGDAEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006872
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006872.5
• RefSeq Size: 1710 bp
• RefSeq ORF: 1437 bp
• Locus ID: 11036
• UniProt ID: Q9UNN4
• Cytogenetics: 2p16.3
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 52.4 kDa
• Gene Summary: The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Mar 2014]