TM4SF2 (TSPAN7) (NM_004615) Human Tagged ORF Clone

CAT#: RC205248

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TSPAN7 (Myc-DDK-tagged)-Human tetraspanin 7 (TSPAN7)



  "NM_004615" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TSPAN7
Synonyms A15; CCG-B7; CD231; DXS1692E; MRX58; MXS1; TALLA-1; TM4SF2; TM4SF2b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205248 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCGAGGAGAATGGAGACCAAACCTGTGATAACCTGTCTCAAAACCCTCCTCATCATCTACTCCT
TCGTCTTCTGGATCACTGGGGTGATCCTGCTGGCTGTTGGAGTCTGGGGCAAACTTACTCTGGGCACCTA
TATCTCCCTTATTGCCAAGAACTCCACAAATGCTCCCTATGTGCTCATCGGAACTGGCACCACTATTGTT
GTCTTTGGCCTGTTTGGATGCTTTGCTACATGTCGTGGTAGCCCATGGATGCTGAAACTGTATGCCATGT
TTCTGTCCCTGGTGTTCCTGGCTGAGCTCGTAGCTGGCATTTCAGGGTTTGTGTTTCGTCATGAGATCAA
GGACACCTTCCTGAGGACTTACACGGACACTATGCAGACTTACAATGGCAATGATGAGAGGAGCCGGGCA
GTGGACCATGTGCAGCGCAGCCTGAGCTGCTGTGGTGTGCAGAACTACACCAACTGGAGCACCAGCCCCT
ACTTCCTGGAGCATGGCATCCCCCCCAGCTGCTGCATGAACGAAACTGATTGTAATCCCCAGGATCTACA
CAATCTGACTGTGGCCGCCACCAAAGTTAACCAGAAGGGTTGTTATGATCTGGTAACTAGTTTCATGGAG
ACTAACATGGGAATCATCGCTGGAGTGGCGTTTGGAATCGCATTCTCCCAGTTAATTGGCATGCTGCTGG
CCTGCTGTCTGTCCCGGTTCATCACGGCCAATCAGTATGAGATGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205248 protein sequence
Red=Cloning site Green=Tags(s)

MASRRMETKPVITCLKTLLIIYSFVFWITGVILLAVGVWGKLTLGTYISLIAKNSTNAPYVLIGTGTTIV
VFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDTMQTYNGNDERSRA
VDHVQRSLSCCGVQNYTNWSTSPYFLEHGIPPSCCMNETDCNPQDLHNLTVAATKVNQKGCYDLVTSFME
TNMGIIAGVAFGIAFSQLIGMLLACCLSRFITANQYEMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004615
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004615.4
RefSeq Size 1816 bp
RefSeq ORF 750 bp
Locus ID 7102
UniProt ID P41732
Cytogenetics Xp11.4
Domains transmembrane4
Protein Families Druggable Genome, Transmembrane
MW 27.6 kDa
Gene Summary The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. It is known to complex with integrins. This gene is associated with X-linked cognitive disability and neuropsychiatric diseases such as Huntington's chorea, fragile X syndrome and myotonic dystrophy. [provided by RefSeq, Jul 2008]

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