MMD (NM_012329) Human Tagged ORF Clone

CAT#: RC205241

MMD (Myc-DDK-tagged)-Human monocyte to macrophage differentiation-associated (MMD)

Product Images

Western blot validation of overexpression lysate (Cat# LY415826) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205241 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MMD
• Synonyms: MMA; MMD1; PAQR11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205241 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTTCAAGAATCGATTCCAGCGGTTCATGAACCATCGAGCTCCAGCCAATGGCCGCTACAAGCCAA
  CTTGCTATGAACATGCTGCTAACTGTTACACACACGCATTCCTCATTGTTCCGGCCATCGTGGGCAGTGC
  CCTCCTCCATCGGCTGTCTGATGACTGCTGGGAAAAGATAACAGCATGGATTTATGGAATGGGACTCTGT
  GCCCTCTTCATCGTTTCTACAGTATTTCACATTGTATCATGGAAAAAGAGCCACTTAAGGACAGTGGAGC
  ATTGTTTTCACATGTGTGATAGAATGGTTATCTATTTCTTCATTGCTGCTTCTTATGCTCCATGGTTAAA
  TCTTCGTGAACTTGGACCCCTGGCATCTCATATGCGTTGGTTTATCTGGCTCATGGCAGCTGGAGGAACC
  ATTTATGTATTTCTCTACCATGAAAAATATAAGGTGGTTGAACTCTTTTTCTATCTCACAATGGGATTCT
  CTCCAGCCTTGGTGGTGACATCAATGAACAACACCGATGGACTTCAGGAACTTGCCTGTGGGGGCTTAAT
  TTATTGCTTGGGAGTTGTGTTCTTCAAGAGTGATGGCATCATTCCATTTGCCCACGCCATCTGGCACCTG
  TTTGTGGCCACGGCAGCTGCAGTGCATTACTACGCCATTTGGAAATACCTTTACCGAAGTCCTACGGACT
  TTATGCGGCATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205241 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRFKNRFQRFMNHRAPANGRYKPTCYEHAANCYTHAFLIVPAIVGSALLHRLSDDCWEKITAWIYGMGLC
  ALFIVSTVFHIVSWKKSHLRTVEHCFHMCDRMVIYFFIAASYAPWLNLRELGPLASHMRWFIWLMAAGGT
  IYVFLYHEKYKVVELFFYLTMGFSPALVVTSMNNTDGLQELACGGLIYCLGVVFFKSDGIIPFAHAIWHL
  FVATAAAVHYYAIWKYLYRSPTDFMRHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012329
• ORF Size: 714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012329.3
• RefSeq Size: 2723 bp
• RefSeq ORF: 717 bp
• Locus ID: 23531
• UniProt ID: Q15546
• Cytogenetics: 17q22
• Domains: UPF0073
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 27.7 kDa
• Gene Summary: This protein is expressed by in vitro differentiated macrophages but not freshly isolated monocytes. Although sequence analysis identifies seven potential transmembrane domains, this protein has little homology to G-protein receptors and it has not been positively identified as a receptor. A suggested alternative function is that of an ion channel protein in maturing macrophages. [provided by RefSeq, Jul 2008]