TBK1 (NM_013254) Human Tagged ORF Clone

CAT#: RC205238

TBK1 (Myc-DDK-tagged)-Human TANK-binding kinase 1 (TBK1)

Product Images

Circular map for RC205238

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  Western blot validation of overexpression lysate (Cat# LY415713) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205238 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TBK1 protein (Cat# TP305238). The protein was produced from HEK293T cells transfected with TBK1 cDNA clone (Cat# RC205238) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBK1
• Synonyms: FTDALS4; IIAE8; NAK; T2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205238 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGCACTTCTAATCATCTGTGGCTTTTATCTGATATTTTAGGCCAAGGAGCTACTGCAAATGTCT
  TTCGTGGAAGACATAAGAAAACTGGTGATTTATTTGCTATCAAAGTATTTAATAACATAAGCTTCCTTCG
  TCCAGTGGATGTTCAAATGAGAGAATTTGAAGTGTTGAAAAAACTCAATCACAAAAATATTGTCAAATTA
  TTTGCTATTGAAGAGGAGACAACAACAAGACATAAAGTACTTATTATGGAATTTTGTCCATGTGGGAGTT
  TATACACTGTTTTAGAAGAACCTTCTAATGCCTATGGACTACCAGAATCTGAATTCTTAATTGTTTTGCG
  AGATGTGGTGGGTGGAATGAATCATCTACGAGAGAATGGTATAGTGCACCGTGATATCAAGCCAGGAAAT
  ATCATGCGTGTTATAGGGGAAGATGGACAGTCTGTGTACAAACTCACAGATTTTGGTGCAGCTAGAGAAT
  TAGAAGATGATGAGCAGTTTGTTTCTCTGTATGGCACAGAAGAATATTTGCACCCTGATATGTATGAGAG
  AGCAGTGCTAAGAAAAGATCATCAGAAGAAATATGGAGCAACAGTTGATCTTTGGAGCATTGGGGTAACA
  TTTTACCATGCAGCTACTGGATCACTGCCATTTAGACCCTTTGAAGGGCCTCGTAGGAATAAAGAAGTGA
  TGTATAAAATAATTACAGGAAAGCCTTCTGGTGCAATATCTGGAGTACAGAAAGCAGAAAATGGACCAAT
  TGACTGGAGTGGAGACATGCCTGTTTCTTGCAGTCTTTCTCGGGGTCTTCAGGTTCTACTTACCCCTGTT
  CTTGCAAACATCCTTGAAGCAGATCAGGAAAAGTGTTGGGGTTTTGACCAGTTTTTTGCAGAAACTAGTG
  ATATACTTCACCGAATGGTAATTCATGTTTTTTCGCTACAACAAATGACAGCTCATAAGATTTATATACA
  TAGCTATAATACTGCTACTATATTTCATGAACTGGTATATAAACAAACCAAAATTATTTCTTCAAATCAA
  GAACTTATCTACGAAGGGCGACGCTTAGTCTTAGAACCTGGAAGGCTGGCACAACATTTCCCTAAAACTA
  CTGAGGAAAACCCTATATTTGTAGTAAGCCGGGAACCTCTGGATACCATAGGATTAATATATGAAAAAAT
  TTCCCTCCCTAAAGTACATCCACGTTATGATTTAGACGGGGATGCTAGCATGGCTAAGGCAATAACAGGG
  GTTGTGTGTTATGCCTGCAGAATTGCCAGTACCTTACTGCTTTATCAGGAATTAATGCGAAAGGGGATAC
  GATGGCTGATTGAATTAATTAAAGATGATTACAATGAAACTGTTCACAAAAAGACAGAAGTTGTGATCAC
  ATTGGATTTCTGTATCAGAAACATTGAAAAAACTGTGAAAGTATATGAAAAGTTGATGAAGATCAACCTG
  GAAGCGGCAGAGTTAGGTGAAATTTCAGACATACACACCAAATTGTTGAGACTTTCCAGTTCTCAGGGAA
  CAATAGAAACCAGTCTTCAGGATATCGACAGCAGATTATCTCCAGGTGGATCACTGGCAGACGCATGGGC
  ACATCAAGAAGGCACTCATCCGAAAGACAGAAATGTAGAAAAACTACAAGTCCTGTTAAATTGCATGACA
  GAGATTTACTATCAGTTCAAAAAAGACCAAGCAGAACGTAGATTAGCTTATAATGAAGAACAAATCCACA
  AATTTGATAAGCAAAAACTGTATTACCATGCCACAAAAGCTATGACGCACTTTACAGATGAATGTGTTAA
  AAAGTATGAGGCATTTTTGAATAAGTCAGAAGAATGGATAAGAAAGATGCTTCATCTTAGGAAACAGTTA
  TTATCGCTGACTAATCAGTGTTTTGATATTGAAGAAGAAGTATCAAAATATCAAGAATATACTAATGAGT
  TACAAGAAACTCTGCCTCAGAAAATGTTTACAGCTTCCAGTGGAATCAAACATACCATGACCCCAATTTA
  TCCAAGTTCTAACACATTAGTAGAAATGACTCTTGGTATGAAGAAATTAAAGGAAGAGATGGAAGGGGTG
  GTTAAAGAACTTGCTGAAAATAACCACATTTTAGAAAGGTTTGGCTCTTTAACCATGGATGGTGGCCTTC
  GCAACGTTGACTGTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205238 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKL
  FAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGN
  IMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVT
  FYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPV
  LANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQ
  ELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLDTIGLIYEKISLPKVHPRYDLDGDASMAKAITG
  VVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINL
  EAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMT
  EIYYQFKKDQAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQL
  LSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGV
  VKELAENNHILERFGSLTMDGGLRNVDCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013254
• ORF Size: 2187 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013254.4
• RefSeq Size: 3098 bp
• RefSeq ORF: 2190 bp
• Locus ID: 29110
• UniProt ID: Q9UHD2
• Cytogenetics: 12q14.2
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• MW: 83.6 kDa
• Gene Summary: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]