KLHL11 (NM_018143) Human Tagged ORF Clone

CAT#: RC205228

KLHL11 (Myc-DDK-tagged)-Human kelch-like 11 (Drosophila) (KLHL11)

Product Images

Circular map for RC205228

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  Western blot validation of overexpression lysate (Cat# LY413273) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205228 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KLHL11 protein (Cat# TP305228). The protein was produced from HEK293T cells transfected with KLHL11 cDNA clone (Cat# RC205228) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLHL11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205228 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCGGCAGTGGCGGCGGCGGCGGCGGCGGCCGCGGCTGCATCTCTTCAGGTACTGGAGATGG
  AGAGCATGGAGACGGCCGCCGCCGGCTCGGCAGGACTGGCCGCCGAGGTCCGAGGCAGCGGCACGGTGGA
  CTTCGGGCCTGGGCCGGGGATCTCTGCAATGGAGGCGAGCGGGGGCGATCCGGGCCCAGAAGCCGAGGAT
  TTCGAGTGCAGCTCTCACTGCTCAGAGCTGTCCTGGCGGCAGAACGAGCAGCGGCGCCAGGGCCTCTTCT
  GCGACATTACCCTGTGCTTCGGCGGGGCTGGAGGCCGCGAGTTCCGGGCCCACCGCTCGGTACTGGCTGC
  CGCCACCGAGTACTTCACGCCCCTGCTCTCGGGCCAGTTTTCCGAGTCCCGCTCGGGACGGGTGGAGATG
  CGCAAGTGGAGCTCCGAGCCGGGGCCCGAACCCGACACAGTGGAAGCCGTAATCGAGTACATGTACACCG
  GGCGCATCCGCGTCAGCACGGGCAGCGTGCACGAGGTGCTGGAGTTGGCCGACAGGTTCCTACTCATTCG
  TTTAAAAGAATTTTGTGGAGAATTTCTCAAGAAAAAACTTCATCTCTCAAATTGTGTGGCAATTCATAGC
  TTAGCACACATGTACACCCTGAGCCAACTTGCTCTGAAGGCTGCTGATATGATACGGAGAAATTTCCACA
  AAGTGATTCAGGATGAAGAATTTTATACGTTACCTTTCCATCTCATTAGAGACTGGCTTTCAGATTTGGA
  AATTACTGTTGATTCTGAAGAGGTTCTCTTTGAAACCGTTTTGAAATGGGTTCAGAGAAATGCTGAAGAG
  AGAGAGAGATACTTTGAAGAACTTTTTAAATTGCTCAGGTTGTCCCAGATGAAACCTACCTACCTTACTC
  GACATGTCAAACCAGAGAGGCTGGTAGCCAATAATGAAGTTTGTGTCAAGTTGGTCGCTGACGCAGTGGA
  GAGACATGCTCTGAGAGCTGAGAATATACAATCTGGCACATGCCAGCACCCCACTTCTCATGTGTCACTA
  TTGCCTCGTTATGGGCAAAACATGGATGTGATCATGGTTATTGGAGGTGTGTCAGAAGGAGGGGACTATT
  TAAGTGAATGTGTGGGATATTTTGTTGATGAGGACAGATGGGTAAATCTGCCACATATTCATAATCACCT
  CGATGGACATGCTGTTGCAGTAACAGAATCCTACGTGTATGTTGCTGGATCAATGGAGCCAGGGTTTGCT
  AAAACTGTAGAAAGGTATAACCCAAATTTGAATACATGGGAACATGTTTGTAGTCTGATGACAAGAAAGC
  ATTCTTTTGGACTAACAGAAGTCAAAGGGAAGCTCTATAGCATTGGAGGACACGGCAACTTTAGTCCTGG
  TTTTAAAGATGTGACTGTTTATAATCCTGAGCTTGATAAATGGCACAACTTGGAATCGGCACCAAAGATT
  CTTCGAGATGTCAAAGCACTAGCCATTGAAGACCGGTTTGTATACATTGCCGCCCGCACTCCTGTAGACC
  GGGACACTGAAGATGGATTAAAGGCTGTAATTACTTGCTATGATACAGAGACTCGACAGTGGCAAGATGT
  GGAATCTTTGCCGCTTATTGACAATTACTGCTTTTTCCAAATGTCTGTGGTCAATTCAAACTTTTATCAG
  ACAGCATCATGTTGTCCCAAGAGTTATTGTTTAGAAAACGAAGAGGCAGTAAGAAAAATTGCCAGCCAAG
  TGTCTGATGAGATCCTTGAAAGCTTGCCTCCAGAAGTCCTAAGCATCGAAGGAGCAGCCATTTGCTATTA
  CAAAGATGATGTCTTCATTATAGGAGGCTGGAAAAACAGTGATGATATTGATAAACAGTATCGGAAAGAA
  GCCTACCGATATTGTGCGGAGAGGAAGAGGTGGATGCTTCTTCCTCCTATGCCACAACCTCGTTGTAGAG
  CCACTGCTTGTCACGTGAGGATCCCGTACCGGTACTTGCATGGCACACAGAGATACCCTATGCCTCAAAA
  CCTGATGTGGCAGAAGGACCGCATCAGACAGATGCAAGAGATACATCGTCACGCCCTGAACATGAGGCGA
  GTGCCAAGCTCTCAGATTGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205228 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAVAAAAAAAAAASLQVLEMESMETAAAGSAGLAAEVRGSGTVDFGPGPGISAMEASGGDPGPEAED
  FECSSHCSELSWRQNEQRRQGLFCDITLCFGGAGGREFRAHRSVLAAATEYFTPLLSGQFSESRSGRVEM
  RKWSSEPGPEPDTVEAVIEYMYTGRIRVSTGSVHEVLELADRFLLIRLKEFCGEFLKKKLHLSNCVAIHS
  LAHMYTLSQLALKAADMIRRNFHKVIQDEEFYTLPFHLIRDWLSDLEITVDSEEVLFETVLKWVQRNAEE
  RERYFEELFKLLRLSQMKPTYLTRHVKPERLVANNEVCVKLVADAVERHALRAENIQSGTCQHPTSHVSL
  LPRYGQNMDVIMVIGGVSEGGDYLSECVGYFVDEDRWVNLPHIHNHLDGHAVAVTESYVYVAGSMEPGFA
  KTVERYNPNLNTWEHVCSLMTRKHSFGLTEVKGKLYSIGGHGNFSPGFKDVTVYNPELDKWHNLESAPKI
  LRDVKALAIEDRFVYIAARTPVDRDTEDGLKAVITCYDTETRQWQDVESLPLIDNYCFFQMSVVNSNFYQ
  TASCCPKSYCLENEEAVRKIASQVSDEILESLPPEVLSIEGAAICYYKDDVFIIGGWKNSDDIDKQYRKE
  AYRYCAERKRWMLLPPMPQPRCRATACHVRIPYRYLHGTQRYPMPQNLMWQKDRIRQMQEIHRHALNMRR
  VPSSQIEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018143
• ORF Size: 2124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018143.3
• RefSeq Size: 2326 bp
• RefSeq ORF: 2127 bp
• Locus ID: 55175
• UniProt ID: Q9NVR0
• Cytogenetics: 17q21.2
• Domains: BTB, Kelch
• Protein Families: Secreted Protein
• MW: 80.1 kDa
• Gene Summary: Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.[UniProtKB/Swiss-Prot Function]