KLHL11 (NM_018143) Human Tagged ORF Clone

CAT#: RC205228

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KLHL11 (Myc-DDK-tagged)-Human kelch-like 11 (Drosophila) (KLHL11)



  "NM_018143" in other vectors (4)

Reconstitution Protocol

USD 648.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KLHL11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205228 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCGGCAGTGGCGGCGGCGGCGGCGGCGGCCGCGGCTGCATCTCTTCAGGTACTGGAGATGG
AGAGCATGGAGACGGCCGCCGCCGGCTCGGCAGGACTGGCCGCCGAGGTCCGAGGCAGCGGCACGGTGGA
CTTCGGGCCTGGGCCGGGGATCTCTGCAATGGAGGCGAGCGGGGGCGATCCGGGCCCAGAAGCCGAGGAT
TTCGAGTGCAGCTCTCACTGCTCAGAGCTGTCCTGGCGGCAGAACGAGCAGCGGCGCCAGGGCCTCTTCT
GCGACATTACCCTGTGCTTCGGCGGGGCTGGAGGCCGCGAGTTCCGGGCCCACCGCTCGGTACTGGCTGC
CGCCACCGAGTACTTCACGCCCCTGCTCTCGGGCCAGTTTTCCGAGTCCCGCTCGGGACGGGTGGAGATG
CGCAAGTGGAGCTCCGAGCCGGGGCCCGAACCCGACACAGTGGAAGCCGTAATCGAGTACATGTACACCG
GGCGCATCCGCGTCAGCACGGGCAGCGTGCACGAGGTGCTGGAGTTGGCCGACAGGTTCCTACTCATTCG
TTTAAAAGAATTTTGTGGAGAATTTCTCAAGAAAAAACTTCATCTCTCAAATTGTGTGGCAATTCATAGC
TTAGCACACATGTACACCCTGAGCCAACTTGCTCTGAAGGCTGCTGATATGATACGGAGAAATTTCCACA
AAGTGATTCAGGATGAAGAATTTTATACGTTACCTTTCCATCTCATTAGAGACTGGCTTTCAGATTTGGA
AATTACTGTTGATTCTGAAGAGGTTCTCTTTGAAACCGTTTTGAAATGGGTTCAGAGAAATGCTGAAGAG
AGAGAGAGATACTTTGAAGAACTTTTTAAATTGCTCAGGTTGTCCCAGATGAAACCTACCTACCTTACTC
GACATGTCAAACCAGAGAGGCTGGTAGCCAATAATGAAGTTTGTGTCAAGTTGGTCGCTGACGCAGTGGA
GAGACATGCTCTGAGAGCTGAGAATATACAATCTGGCACATGCCAGCACCCCACTTCTCATGTGTCACTA
TTGCCTCGTTATGGGCAAAACATGGATGTGATCATGGTTATTGGAGGTGTGTCAGAAGGAGGGGACTATT
TAAGTGAATGTGTGGGATATTTTGTTGATGAGGACAGATGGGTAAATCTGCCACATATTCATAATCACCT
CGATGGACATGCTGTTGCAGTAACAGAATCCTACGTGTATGTTGCTGGATCAATGGAGCCAGGGTTTGCT
AAAACTGTAGAAAGGTATAACCCAAATTTGAATACATGGGAACATGTTTGTAGTCTGATGACAAGAAAGC
ATTCTTTTGGACTAACAGAAGTCAAAGGGAAGCTCTATAGCATTGGAGGACACGGCAACTTTAGTCCTGG
TTTTAAAGATGTGACTGTTTATAATCCTGAGCTTGATAAATGGCACAACTTGGAATCGGCACCAAAGATT
CTTCGAGATGTCAAAGCACTAGCCATTGAAGACCGGTTTGTATACATTGCCGCCCGCACTCCTGTAGACC
GGGACACTGAAGATGGATTAAAGGCTGTAATTACTTGCTATGATACAGAGACTCGACAGTGGCAAGATGT
GGAATCTTTGCCGCTTATTGACAATTACTGCTTTTTCCAAATGTCTGTGGTCAATTCAAACTTTTATCAG
ACAGCATCATGTTGTCCCAAGAGTTATTGTTTAGAAAACGAAGAGGCAGTAAGAAAAATTGCCAGCCAAG
TGTCTGATGAGATCCTTGAAAGCTTGCCTCCAGAAGTCCTAAGCATCGAAGGAGCAGCCATTTGCTATTA
CAAAGATGATGTCTTCATTATAGGAGGCTGGAAAAACAGTGATGATATTGATAAACAGTATCGGAAAGAA
GCCTACCGATATTGTGCGGAGAGGAAGAGGTGGATGCTTCTTCCTCCTATGCCACAACCTCGTTGTAGAG
CCACTGCTTGTCACGTGAGGATCCCGTACCGGTACTTGCATGGCACACAGAGATACCCTATGCCTCAAAA
CCTGATGTGGCAGAAGGACCGCATCAGACAGATGCAAGAGATACATCGTCACGCCCTGAACATGAGGCGA
GTGCCAAGCTCTCAGATTGAATGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205228 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAVAAAAAAAAAASLQVLEMESMETAAAGSAGLAAEVRGSGTVDFGPGPGISAMEASGGDPGPEAED
FECSSHCSELSWRQNEQRRQGLFCDITLCFGGAGGREFRAHRSVLAAATEYFTPLLSGQFSESRSGRVEM
RKWSSEPGPEPDTVEAVIEYMYTGRIRVSTGSVHEVLELADRFLLIRLKEFCGEFLKKKLHLSNCVAIHS
LAHMYTLSQLALKAADMIRRNFHKVIQDEEFYTLPFHLIRDWLSDLEITVDSEEVLFETVLKWVQRNAEE
RERYFEELFKLLRLSQMKPTYLTRHVKPERLVANNEVCVKLVADAVERHALRAENIQSGTCQHPTSHVSL
LPRYGQNMDVIMVIGGVSEGGDYLSECVGYFVDEDRWVNLPHIHNHLDGHAVAVTESYVYVAGSMEPGFA
KTVERYNPNLNTWEHVCSLMTRKHSFGLTEVKGKLYSIGGHGNFSPGFKDVTVYNPELDKWHNLESAPKI
LRDVKALAIEDRFVYIAARTPVDRDTEDGLKAVITCYDTETRQWQDVESLPLIDNYCFFQMSVVNSNFYQ
TASCCPKSYCLENEEAVRKIASQVSDEILESLPPEVLSIEGAAICYYKDDVFIIGGWKNSDDIDKQYRKE
AYRYCAERKRWMLLPPMPQPRCRATACHVRIPYRYLHGTQRYPMPQNLMWQKDRIRQMQEIHRHALNMRR
VPSSQIEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018143
ORF Size 2124 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018143.3
RefSeq Size 2326 bp
RefSeq ORF 2127 bp
Locus ID 55175
UniProt ID Q9NVR0
Cytogenetics 17q21.2
Domains BTB, Kelch
Protein Families Secreted Protein
MW 80.1 kDa
Gene Summary Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.[UniProtKB/Swiss-Prot Function]

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