OGDHL (NM_018245) Human Tagged ORF Clone

CAT#: RC205225

OGDHL (Myc-DDK-tagged)-Human oxoglutarate dehydrogenase-like (OGDHL), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC205225

• 

  Western blot validation of overexpression lysate (Cat# LY413186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205225 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OGDHL protein (Cat# TP305225). The protein was produced from HEK293T cells transfected with OGDHL cDNA clone (Cat# RC205225) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OGDHL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205225 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAGCTGAGGCTGCTGCCGTCCCGTCTTGGGGTGCAGGCTGCGAGGCTCCTGGCTGCACATGACG
  TCCCGGTGTTTGGCTGGCGCAGCAGGTCCTCCGGGCCACCGGCCACCTTCCCAAGCAGCAAAGGTGGAGG
  CGGCTCCAGTTACATGGAGGAGATGTACTTCGCCTGGTTGGAAAACCCCCAGAGTGTCCACAAGTCCTGG
  GACAGCTTCTTCAGGGAAGCCAGCGAGGAAGCCTTTTCTGGCTCTGCTCAGCCACGGCCCCCTTCTGTTG
  TCCATGAGAGCAGGTCTGCAGTCTCAAGTCGGACCAAGACCAGCAAATTGGTGGAGGACCACCTGGCTGT
  GCAGTCCCTGATCCGGGCCTACCAGATCCGGGGTCACCATGTGGCCCAGCTGGACCCCCTGGGCATTCTG
  GATGCAGACCTGGACTCCTTTGTGCCCTCAGACTTGATCACAACCATTGATAAACTGGCCTTCTATGACC
  TTCAGGAGGCTGACCTTGATAAGGAGTTCCAGCTGCCGACAACCACCTTCATTGGGGGCTCTGAAAACAC
  CCTTTCTCTGCGGGAGATCATTCGGCGCCTGGAGAACACCTACTGCCAGCACATTGGCCTGGAGTTCATG
  TTCATCAACGATGTGGAGCAGTGCCAGTGGATCCGGCAGAAGTTTGAGACCCCTGGTGTGATGCAGTTCT
  CCAGCGAGGAGAAGCGGACCCTGCTGGCCCGGCTAGTGCGCTCCATGAGGTTTGAAGACTTCCTGGCCCG
  GAAATGGTCCTCAGAGAAGCGGTTTGGCCTGGAGGGCTGTGAAGTGATGATTCCTGCCCTCAAGACCATC
  ATCGACAAATCCAGCGAGATGGGGATTGAGAATGTCATCTTGGGGATGCCACACAGGGGAAGGCTGAACG
  TGCTGGCCAACGTGATCCGCAAGGACCTGGAGCAGATCTTCTGCCAGTTTGACCCCAAGCTGGAGGCGGC
  GGACGAGGGCTCCGGGGATGTCAAGTACCACCTGGGCATGTACCATGAGAGGATCAACCGCGTCACCAAC
  CGGAACATCACTCTGTCGCTGGTTGCCAACCCCTCCCACCTGGAGGCAGTGGACCCTGTGGTGCAGGGGA
  AGACAAAGGCAGAGCAGTTCTACCGTGGAGATGCCCAGGGCAAGAAGGTCATGTCCATCCTGGTTCATGG
  GGACGCCGCCTTTGCTGGCCAGGGCGTGGTATATGAGACCTTCCACCTGAGCGACCTGCCCTCCTACACG
  ACCAATGGTACCGTGCACGTCGTCGTCAACAACCAGATTGGATTCACCACAGACCCCCGAATGGCCCGCT
  CCTCACCATACCCGACCGACGTGGCCCGGGTGGTCAATGCGCCTATCTTCCATGTGAATGCAGATGACCC
  AGAGGCTGTGATATATGTGTGCAGTGTGGCAGCCGAATGGAGAAACACTTTCAACAAAGATGTTGTCGTG
  GACCTGGTCTGTTACCGCCGGCGTGGCCACAATGAGATGGACGAGCCCATGTTCACCCAGCTGCTCATGT
  ACAAGCAGATCCACAGACAGGTGCCTGTGCTGAAGAAGTACGCAGACAAGCTGATTGCCGAGGGCACAGT
  CACCCTGCAGGAGTTTGAGGAAGAAATTGCCAAATACGACCGGATCTGTGAGGAGGCTTATGGCAGGTCC
  AAGGATAAAAAGATTCTGCATATAAAGCACTGGTTGAACTCCCCCTGGCCTGGCTTCTTCAACGTAGATG
  GGGAGCCCAAGAGCATGACATGCCCAGCCACGGGGATCCCTGAGGACATGCTCACCCACATCGGCAGTGT
  GGCCAGCTCTGTGCCCCTGGAGGACTTTAAGATCCACACTGGCCTCTCTCGCATTCTGCGGGGCCGTGCG
  GACATGACCAAGAACCGGACGGTGGACTGGGCGTTGGCAGAGTACATGGCCTTTGGCTCCCTGCTGAAGG
  AAGGCATCCACGTGCGGCTCAGCGGGCAGGATGTGGAGAGGGGCACATTCAGTCACCGGCACCATGTTCT
  CCATGACCAGGAGGTTGACCGCAGGACGTGTGTGCCTATGAATCATCTCTGGCCTGACCAGGCCCCGTAC
  ACCGTGTGCAACAGCTCCCTCTCGGAGTACGGAGTCCTGGGCTTTGAGCTGGGCTATGCCATGGCCAGCC
  CCAATGCCCTGGTCCTCTGGGAGGCCCAGTTTGGGGACTTCCACAACACGGCCCAGTGCATCATCGACCA
  GTTCATCAGCACCGGCCAGGCCAAGTGGGTGCGGCATAATGGCATTGTGCTGCTGCTGCCCCATGGCATG
  GAAGGCATGGGCCCAGAGCACTCGTCAGCGAGGCCCGAAAGGTTCCTGCAGATGAGCAATGATGACTCGG
  ATGCCTACCCTGCATTCACCAAGGACTTCGAGGTGAGCCAGCTCTATGACTGCAACTGGATCGTGGTCAA
  CTGCTCCACACCGGCCAACTACTTCCACGTGCTGCGCCGGCAGATCCTGCTGCCCTTCCGCAAGCCGCTG
  ATTATCTTCACACCTAAATCTCTGCTGAGGCACCCAGAGGCCAAGTCCAGCTTTGACCAAATGGTATCCG
  GGACCAGCTTCCAGCGGGTGATTCCTGAAGATGGGGCCGCAGCACGGGCCCCTGAGCAGGTGCAGCGGCT
  CATCTTCTGCACGGGAAAGGTGTACTATGACCTGGTGAAGGAGCGGAGCAGCCAGGACCTGGAGGAGAAA
  GTGGCCATCACGCGCCTGGAGCAGATCTCTCCATTCCCCTTCGACCTGATCAAGCAGGAGGCAGAGAAGT
  ACCCAGGTGCGGAGCTGGCCTGGTGTCAGGAGGAGCACAAGAACATGGGCTACTATGACTACATCAGCCC
  ACGCTTCATGACCATCCTGAGGCGCGCACGGCCCATATGGTATGTTGGCCGGGACCCAGCGGCTGCACCA
  GCCACAGGAAACAGGAACACTCACCTGGTGTCACTGAAGAAGTTTCTGGATACTGCCTTCAATCTCCAGG
  CCTTTGAGGGCAAGACATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205225 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQLRLLPSRLGVQAARLLAAHDVPVFGWRSRSSGPPATFPSSKGGGGSSYMEEMYFAWLENPQSVHKSW
  DSFFREASEEAFSGSAQPRPPSVVHESRSAVSSRTKTSKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGIL
  DADLDSFVPSDLITTIDKLAFYDLQEADLDKEFQLPTTTFIGGSENTLSLREIIRRLENTYCQHIGLEFM
  FINDVEQCQWIRQKFETPGVMQFSSEEKRTLLARLVRSMRFEDFLARKWSSEKRFGLEGCEVMIPALKTI
  IDKSSEMGIENVILGMPHRGRLNVLANVIRKDLEQIFCQFDPKLEAADEGSGDVKYHLGMYHERINRVTN
  RNITLSLVANPSHLEAVDPVVQGKTKAEQFYRGDAQGKKVMSILVHGDAAFAGQGVVYETFHLSDLPSYT
  TNGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVIYVCSVAAEWRNTFNKDVVV
  DLVCYRRRGHNEMDEPMFTQLLMYKQIHRQVPVLKKYADKLIAEGTVTLQEFEEEIAKYDRICEEAYGRS
  KDKKILHIKHWLNSPWPGFFNVDGEPKSMTCPATGIPEDMLTHIGSVASSVPLEDFKIHTGLSRILRGRA
  DMTKNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQEVDRRTCVPMNHLWPDQAPY
  TVCNSSLSEYGVLGFELGYAMASPNALVLWEAQFGDFHNTAQCIIDQFISTGQAKWVRHNGIVLLLPHGM
  EGMGPEHSSARPERFLQMSNDDSDAYPAFTKDFEVSQLYDCNWIVVNCSTPANYFHVLRRQILLPFRKPL
  IIFTPKSLLRHPEAKSSFDQMVSGTSFQRVIPEDGAAARAPEQVQRLIFCTGKVYYDLVKERSSQDLEEK
  VAITRLEQISPFPFDLIKQEAEKYPGAELAWCQEEHKNMGYYDYISPRFMTILRRARPIWYVGRDPAAAP
  ATGNRNTHLVSLKKFLDTAFNLQAFEGKTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018245
• ORF Size: 3030 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_018245.3
• RefSeq Size: 3774 bp
• RefSeq ORF: 3033 bp
• Locus ID: 55753
• UniProt ID: Q9ULD0
• Cytogenetics: 10q11.23
• Protein Pathways: Citrate cycle (TCA cycle), Lysine degradation, Metabolic pathways, Tryptophan metabolism
• MW: 114.5 kDa
• Gene Summary: The protein encoded by this gene is similar to oxoglutarate dehydrogenase (OGDH) of the OGDH complex, which degrades glucose and glutamate. This gene encodes several isoforms, including some that appear to localize to mitochondria. The encoded protein down-regulates the AKT signaling cascade and can suppress the growth of cervical cancer cells. [provided by RefSeq, Dec 2016]