PRMT8 (NM_019854) Human Tagged ORF Clone

CAT#: RC205188

PRMT8 (Myc-DDK-tagged)-Human protein arginine methyltransferase 8 (PRMT8)

Product Images

Western blot validation of overexpression lysate (Cat# LY402741) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205188 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRMT8 protein (Cat# TP305188). The protein was produced from HEK293T cells transfected with PRMT8 cDNA clone (Cat# RC205188) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRMT8
• Synonyms: HRMT1L3; HRMT1L4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205188 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTGAGGAGGAAAATGGCGGAGAACGCGGCCGAGAGCA
  CCGAGGTGAACAGCCCCCCCTCCCAGCCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCA
  TCATGTGTCCACTCAACCCAGCTGCCCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATG
  ACCTCGAGAGATTATTACTTCGACTCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGG
  TGCGGACTCTCACTTACCGGAACTCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGA
  TGTGGGGAGTGGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATC
  GAATGCTCCAGTATTTCTGACTACTCACAGAAGATCATTAAGGCCAACCACTTGGACAACATCATCACCA
  TATTTAAGGGTAAAGTGGAAGAGGTGGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGAT
  GGGCTACTGTCTGTTCTATGAGTCCATGCTCAACACGGTGATCTTTGCCAGGGACAAGTGGCTGAAACCT
  GGAGGGCTTATGTTTCCAGACCGGGCAGCTTTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACT
  TCAAAATCCACTGGTGGGAGAATGTCTATGGCTTTGACATGACCTGCATCAGGGACGTGGCCATGAAGGA
  GCCTCTAGTGGACATCGTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATT
  TACACAGTGAAGACGGAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGCAACGACTACG
  TCCACGCCCTGGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAGAAAATGGGGTTTTCCACAGC
  CCCTGATGCTCCCTACACCCACTGGAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGG
  GGGGAGGAAATCTACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAG
  TAGACTTGGATTTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205188 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGMKHSSRCLLLRRKMAENAAESTEVNSPPSQPPQPVVPAKPVQCVHHVSTQPSCPGRGKMSKLLNPEEM
  TSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGI
  ECSSISDYSQKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKP
  GGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDI
  YTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRR
  GEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019854
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019854.5
• RefSeq Size: 2417 bp
• RefSeq ORF: 1185 bp
• Locus ID: 56341
• UniProt ID: Q9NR22
• Cytogenetics: 12p13.32
• Protein Families: Druggable Genome
• MW: 45.3 kDa
• Gene Summary: Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]