PRMT8 (NM_019854) Human Tagged ORF Clone

CAT#: RC205188

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PRMT8 (Myc-DDK-tagged)-Human protein arginine methyltransferase 8 (PRMT8)



Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PRMT8
Synonyms HRMT1L3; HRMT1L4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCATGAAACACTCCTCCCGCTGCCTGCTCCTGAGGAGGAAAATGGCGGAGAACGCGGCCGAGAGCA
CCGAGGTGAACAGCCCCCCCTCCCAGCCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCA
TCATGTGTCCACTCAACCCAGCTGCCCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATG
ACCTCGAGAGATTATTACTTCGACTCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGG
TGCGGACTCTCACTTACCGGAACTCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGA
TGTGGGGAGTGGTACTGGGATCCTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATC
GAATGCTCCAGTATTTCTGACTACTCACAGAAGATCATTAAGGCCAACCACTTGGACAACATCATCACCA
TATTTAAGGGTAAAGTGGAAGAGGTGGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGAT
GGGCTACTGTCTGTTCTATGAGTCCATGCTCAACACGGTGATCTTTGCCAGGGACAAGTGGCTGAAACCT
GGAGGGCTTATGTTTCCAGACCGGGCAGCTTTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACT
TCAAAATCCACTGGTGGGAGAATGTCTATGGCTTTGACATGACCTGCATCAGGGACGTGGCCATGAAGGA
GCCTCTAGTGGACATCGTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATT
TACACAGTGAAGACGGAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGCAACGACTACG
TCCACGCCCTGGTCACCTATTTTAATATTGAATTTACCAAGTGCCACAAGAAAATGGGGTTTTCCACAGC
CCCTGATGCTCCCTACACCCACTGGAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGG
GGGGAGGAAATCTACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAG
TAGACTTGGATTTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205188 protein sequence
Red=Cloning site Green=Tags(s)

MGMKHSSRCLLLRRKMAENAAESTEVNSPPSQPPQPVVPAKPVQCVHHVSTQPSCPGRGKMSKLLNPEEM
TSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGI
ECSSISDYSQKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKP
GGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDI
YTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRR
GEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019854
ORF Size 1182 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019854.5
RefSeq Size 2417 bp
RefSeq ORF 1185 bp
Locus ID 56341
UniProt ID Q9NR22
Cytogenetics 12p13.32
Protein Families Druggable Genome
MW 45.3 kDa
Gene Summary Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
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