DNAJB9 (NM_012328) Human Tagged ORF Clone
DNAJB9 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9)
|Type||Human Tagged ORF Clone|
|Synonyms||ERdj4; MDG-1; MDG1; MST049; MSTP049|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC205128 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205128 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||669 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2538 bp|
|RefSeq ORF||672 bp|
|Protein Families||Druggable Genome, Transmembrane|
|Gene Summary||This gene is a member of the J protein family. J proteins function in many cellular processes by regulating the ATPase activity of 70 kDa heat shock proteins. This gene is a member of the type 2 subgroup of DnaJ proteins. The encoded protein is localized to the endoplasmic reticulum. This protein is induced by endoplasmic reticulum stress and plays a role in protecting stressed cells from apoptosis. [provided by RefSeq, Dec 2010]|
|cDNA Clone Resources|
|RC205128L1||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9), Myc-DDK-tagged||
|RC205128L2||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9), mGFP tagged||
|RC205128L3||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9), Myc-DDK-tagged||
|RC205128L4||Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9), mGFP tagged||
|RG205128||DNAJB9 (GFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9)||
|SC115412||DNAJB9 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9)||
|SC322545||DNAJB9 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9)||