Endo G (ENDOG) (NM_004435) Human Tagged ORF Clone

CAT#: RC205089

ENDOG (Myc-DDK-tagged)-Human endonuclease G (ENDOG), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC205089

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ENDOG (Cat# RC205089, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENDOG(Cat# TA504189). Positive lysates LY417987 (100ug) and LC417987 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417987) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205089 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Endo G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205089 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGCTGCGGGCCGGCCTGACCCTGGCGTTGGGCGCGGGGCTGGGTGCGGTCGTCGAGGGCTGGC
  GGCGGCGGCGGGAGGACGCGCGGGCGGCGCCGGGACTGCTGGGCCGGCTGCCCGTGCTGCCCGTGGCGGC
  GGCAGCCGAGTTGCCCCCTGTGCCCGGGGGACCCCGCGGCCCGGGCGAGCTGGCCAAGTACGGGCTGCCG
  GGGCTGGCGCAGCTCAAGAGCCGCGAGTCGTACGTGCTGTGCTACGACCCGCGCACCCGCGGCGCGCTCT
  GGGTGGTGGAGCAGCTGCGACCCGAGCGTCTCCGCGGCGACGGCGACCGGCGCGAGTGCGACTTCCGCGA
  GGACGACTCGGTGCACGCGTACCACCGTGCCACCAACGCCGACTACCGCGGCAGTGGCTTCGACCGCGGT
  CACCTGGCCGCCGCCGCCAACCACCGCTGGAGCCAGAAGGCCATGGACGACACGTTCTACCTGAGCAACG
  TCGCGCCCCAGGTGCCCCACCTCAACCAGAATGCCTGGAACAACCTGGAGAAATATAGCCGCAGCTTGAC
  CCGCAGCTACCAAAACGTCTATGTCTGCACAGGGCCACTCTTCCTGCCCAGGACAGAGGCTGATGGGAAA
  TCCTACGTAAAGTACCAGGTCATCGGCAAGAACCACGTGGCAGTGCCCACACACTTCTTCAAGGTGCTGA
  TCCTGGAGGCAGCAGGTGGGCAAATTGAGCTCCGCACCTACGTGATGCCCAACGCACCTGTGGATGAGGC
  CATCCCACTGGAGCGCTTCCTGGTGCCCATCGAGAGCATTGAGCGGGCTTCGGGGCTGCTCTTTGTGCCA
  AACATCCTGGCGCGGGCAGGCAGCCTCAAGGCCATCACGGCGGGCAGTAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205089 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRALRAGLTLALGAGLGAVVEGWRRRREDARAAPGLLGRLPVLPVAAAAELPPVPGGPRGPGELAKYGLP
  GLAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDGDRRECDFREDDSVHAYHRATNADYRGSGFDRG
  HLAAAANHRWSQKAMDDTFYLSNVAPQVPHLNQNAWNNLEKYSRSLTRSYQNVYVCTGPLFLPRTEADGK
  SYVKYQVIGKNHVAVPTHFFKVLILEAAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVP
  NILARAGSLKAITAGSK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004435
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004435.2, NP_004426.2
• RefSeq Size: 1145 bp
• RefSeq ORF: 894 bp
• Locus ID: 2021
• UniProt ID: Q14249
• Cytogenetics: 9q34.11
• Domains: Endonuclease
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis
• MW: 32.6 kDa
• Gene Summary: The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008]