Annexin A2 (ANXA2) (NM_001002857) Human Tagged ORF Clone
ANXA2 (Myc-DDK-tagged)-Human annexin A2 (ANXA2), transcript variant 2
|Product Name||Annexin A2 (ANXA2) (NM_001002857) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ANX2; ANX2L4; CAL1H; HEL-S-270; LIP2; LPC2; LPC2D; P36; PAP-IV|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC205081 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205081 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1017 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1644 bp|
|RefSeq ORF||1020 bp|
|Protein Families||Druggable Genome, Secreted Protein, Stem cell - Pluripotency|
|Gene Summary||This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. Annexin A2 expression has been found to correlate with resistance to treatment against various cancer forms. [provided by RefSeq, Dec 2019]|
|The use of this cDNA Clones has been cited in the following citations:|
Cancer-associated fibroblasts promote epithelial-mesenchymal transition and EGFR-TKI resistance of non-small cell lung cancers via HGF/IGF-1/ANXA2 signaling
,Yi, Y;Zeng, S;Wang, Z;Wu, M;Ma, Y;Ye, X;Zhang, B;Liu, H;,
Biochim. Biophys. Acta 2017
,PubMed ID 29253515
|cDNA Clone Resources|
|SC300464||ANXA2 (untagged)-Human annexin A2 (ANXA2), transcript variant 2||
|RG205081||ANXA2 (GFP-tagged) - Human annexin A2 (ANXA2), transcript variant 2||
|RC205081L1||Lenti ORF clone of Human annexin A2 (ANXA2), transcript variant 2, Myc-DDK-tagged||
|RC205081L2||Lenti ORF clone of Human annexin A2 (ANXA2), transcript variant 2, mGFP tagged||
|RC205081L3||Lenti ORF clone of Human annexin A2 (ANXA2), transcript variant 2, Myc-DDK-tagged||
|RC205081L4||Lenti ORF clone of Human annexin A2 (ANXA2), transcript variant 2, mGFP tagged||