SPRYD4 (NM_207344) Human Tagged ORF Clone

CAT#: RC205037

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SPRYD4 (Myc-DDK-tagged)-Human SPRY domain containing 4 (SPRYD4)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SPRYD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205037 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGCTTTTTGCACGTTCTTTGCGCTTGTGCCGCTGGGGAGCCAAACGATTGGGAGTTGCCTCCA
CAGAGGCCCAGAGAGGCGTCAGTTTCAAACTGGAAGAAAAAACCGCCCACAGCAGCCTGGCACTCTTCAG
AGATGATACGGGTGTCAAATATGGCTTGGTGGGATTGGAGCCCACCAAGGTGGCCTTGAATGTGGAGCGC
TTCCGGGAGTGGGCAGTGGTGCTGGCAGACACAGCGGTCACCAGTGGCAGACACTACTGGGAAGTGACAG
TGAAGCGCTCCCAGCAGTTCCGGATAGGAGTGGCAGATGTGGACATGTCCCGGGATAGCTGCATTGGTGT
TGATGATCGTTCCTGGGTGTTCACCTATGCCCAGCGCAAGTGGTACACCATGTTGGCCAACGAGAAAGCC
CCAGTTGAGGGTATTGGGCAGCCAGAGAAGGTGGGGCTGTTGCTGGAGTATGAGGCCCAGAAGCTGAGCC
TGGTGGATGTGAGCCAGGTCTCTGTGGTTCACACGCTACAGACAGATTTCCGGGGTCCAGTGGTGCCTGC
CTTTGCTCTCTGGGATGGGGAGCTGCTGACCCATTCAGGGCTTGAGGTGCCCGAGGGCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205037 protein sequence
Red=Cloning site Green=Tags(s)

MALLFARSLRLCRWGAKRLGVASTEAQRGVSFKLEEKTAHSSLALFRDDTGVKYGLVGLEPTKVALNVER
FREWAVVLADTAVTSGRHYWEVTVKRSQQFRIGVADVDMSRDSCIGVDDRSWVFTYAQRKWYTMLANEKA
PVEGIGQPEKVGLLLEYEAQKLSLVDVSQVSVVHTLQTDFRGPVVPAFALWDGELLTHSGLEVPEGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_207344
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_207344.4
RefSeq Size 2122 bp
RefSeq ORF 624 bp
Locus ID 283377
UniProt ID Q8WW59
Cytogenetics 12q13.3
Protein Families Druggable Genome
MW 23.1 kDa
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