UPRT (NM_145052) Human Tagged ORF Clone

CAT#: RC205030

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UPRT (Myc-DDK-tagged)-Human uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae) (UPRT), transcript variant 1



  "NM_145052" in other vectors (4)

Reconstitution Protocol

USD 300.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol UPRT
Synonyms FUR1; UPP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205030 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGGAGTTACAGTGTCCGGACTCCATGCCCTGTCACAACCAGCAAGTAAACTCTGCCTCAACCC
CAAGTCCCGAGCAGCTGCGACCTGGCGATCTGATCCTGGACCACGCAGGGGGAAACAGAGCCTCCAGGGC
CAAGGTGATTCTCCTCACGGGGTACGCCCATTCTAGCCTGCCGGCCGAGCTGGACTCTGGGGCCTGCGGC
GGCTCCAGCCTCAACTCAGAGGGCAACAGTGGTAGTGGTGACAGTAGCAGCTATGACGCACCAGCTGGCA
ACTCCTTCCTAGAGGACTGCGAACTCTCCCGGCAGATCGGGGCGCAGCTTAAGCTGCTGCCTATGAATGA
TCAGATACGGGAGCTACAGACCATCATCCGGGACAAGACAGCCAGTAGAGGTGACTTCATGTTTTCTGCG
GATCGTTTGATCAGACTTGTTGTGGAAGAGGGATTGAATCAGCTGCCATATAAAGAATGCATGGTGACCA
CTCCAACAGGGTACAAGTATGAAGGAGTGAAATTTGAGAAGGGAAATTGTGGGGTCAGCATAATGAGAAG
CGGTGAGGCAATGGAACAAGGTTTACGAGACTGCTGTCGATCCATACGAATTGGAAAGATCCTGATTCAG
AGTGATGAGGAGACACAAAGAGCCAAAGTATATTATGCCAAATTCCCCCCAGACATTTACCGGAGAAAAG
TCCTTCTGATGTATCCAATTCTCAGCACTGGAAATACTGTAATTGAAGCTGTAAAGGTTCTTATAGAACA
TGGAGTTCAACCCAGTGTTATCATCCTACTCAGTCTGTTCTCCACTCCTCATGGTGCCAAATCAATCATT
CAGGAGTTTCCAGAGATCACAATTTTAACTACTGAAGTTCATCCTGTTGCACCTACACATTTTGGACAGA
AATACTTTGGAACAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205030 protein sequence
Red=Cloning site Green=Tags(s)

MATELQCPDSMPCHNQQVNSASTPSPEQLRPGDLILDHAGGNRASRAKVILLTGYAHSSLPAELDSGACG
GSSLNSEGNSGSGDSSSYDAPAGNSFLEDCELSRQIGAQLKLLPMNDQIRELQTIIRDKTASRGDFMFSA
DRLIRLVVEEGLNQLPYKECMVTTPTGYKYEGVKFEKGNCGVSIMRSGEAMEQGLRDCCRSIRIGKILIQ
SDEETQRAKVYYAKFPPDIYRRKVLLMYPILSTGNTVIEAVKVLIEHGVQPSVIILLSLFSTPHGAKSII
QEFPEITILTTEVHPVAPTHFGQKYFGTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145052
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145052.2
RefSeq Size 2512 bp
RefSeq ORF 930 bp
Locus ID 139596
UniProt ID Q96BW1
Cytogenetics Xq13.3
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pyrimidine metabolism
MW 33.8 kDa
Gene Summary This gene encodes uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. [provided by RefSeq, Nov 2009]

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