UPRT (NM_145052) Human Tagged ORF Clone

CAT#: RC205030

UPRT (Myc-DDK-tagged)-Human uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae) (UPRT), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UPRT (Cat# RC205030, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UPRT(Cat# TA506874). Positive lysates LY408068 (100ug) and LC408068 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY408068) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205030 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UPRT protein (Cat# TP305030). The protein was produced from HEK293T cells transfected with UPRT cDNA clone (Cat# RC205030) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UPRT
• Synonyms: FUR1; UPP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGGAGTTACAGTGTCCGGACTCCATGCCCTGTCACAACCAGCAAGTAAACTCTGCCTCAACCC
  CAAGTCCCGAGCAGCTGCGACCTGGCGATCTGATCCTGGACCACGCAGGGGGAAACAGAGCCTCCAGGGC
  CAAGGTGATTCTCCTCACGGGGTACGCCCATTCTAGCCTGCCGGCCGAGCTGGACTCTGGGGCCTGCGGC
  GGCTCCAGCCTCAACTCAGAGGGCAACAGTGGTAGTGGTGACAGTAGCAGCTATGACGCACCAGCTGGCA
  ACTCCTTCCTAGAGGACTGCGAACTCTCCCGGCAGATCGGGGCGCAGCTTAAGCTGCTGCCTATGAATGA
  TCAGATACGGGAGCTACAGACCATCATCCGGGACAAGACAGCCAGTAGAGGTGACTTCATGTTTTCTGCG
  GATCGTTTGATCAGACTTGTTGTGGAAGAGGGATTGAATCAGCTGCCATATAAAGAATGCATGGTGACCA
  CTCCAACAGGGTACAAGTATGAAGGAGTGAAATTTGAGAAGGGAAATTGTGGGGTCAGCATAATGAGAAG
  CGGTGAGGCAATGGAACAAGGTTTACGAGACTGCTGTCGATCCATACGAATTGGAAAGATCCTGATTCAG
  AGTGATGAGGAGACACAAAGAGCCAAAGTATATTATGCCAAATTCCCCCCAGACATTTACCGGAGAAAAG
  TCCTTCTGATGTATCCAATTCTCAGCACTGGAAATACTGTAATTGAAGCTGTAAAGGTTCTTATAGAACA
  TGGAGTTCAACCCAGTGTTATCATCCTACTCAGTCTGTTCTCCACTCCTCATGGTGCCAAATCAATCATT
  CAGGAGTTTCCAGAGATCACAATTTTAACTACTGAAGTTCATCCTGTTGCACCTACACATTTTGGACAGA
  AATACTTTGGAACAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATELQCPDSMPCHNQQVNSASTPSPEQLRPGDLILDHAGGNRASRAKVILLTGYAHSSLPAELDSGACG
  GSSLNSEGNSGSGDSSSYDAPAGNSFLEDCELSRQIGAQLKLLPMNDQIRELQTIIRDKTASRGDFMFSA
  DRLIRLVVEEGLNQLPYKECMVTTPTGYKYEGVKFEKGNCGVSIMRSGEAMEQGLRDCCRSIRIGKILIQ
  SDEETQRAKVYYAKFPPDIYRRKVLLMYPILSTGNTVIEAVKVLIEHGVQPSVIILLSLFSTPHGAKSII
  QEFPEITILTTEVHPVAPTHFGQKYFGTD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145052
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145052.2
• RefSeq Size: 2512 bp
• RefSeq ORF: 930 bp
• Locus ID: 139596
• UniProt ID: Q96BW1
• Cytogenetics: Xq13.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism
• MW: 33.8 kDa
• Gene Summary: This gene encodes uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. [provided by RefSeq, Nov 2009]